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We narrowed to 745 results for: TRI

Showing: 151 - 200 of 745 results

  1. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...This post was contributed by Alessia Armezzani, scientific communication manager at genOway. A few decades...Hegemann, 1991). These proteins control the flow of electric charges across cell membranes and maintain membrane...neurons have in common? Neuronal stimulations are triggered by the movement of ions across the axon's membrane... is reached and the neuron fires, sending the electrical signal down the axon (Lodish et al., 2000). Most...light activates channelrhodopsins that, in turn, trigger neural excitation, whereas yellow light activates...stimulations, they simultaneously recorded the electrical impulses of discrete subpopulations of this brain...Through a partnership with genOway, we are able to distribute materials containing FLEx technology. Read the...

  2. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...intensity, excitation and emission spectra. The lab of Dimitri Deheyn recently identified a family of GFP proteins... that they can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer...into Golden Gate entry vectors via traditional restriction enzyme cloning, TA cloning, or annealing of oligonucleotides...team at the Institute of Science and Technology Austria, decided to take a novel approach to control for...activity levels and limited sensitivity under the stringently low expression conditions necessary to avoid ...chemogenetic technologies After several years of distributing his Designer Receptors Exclusively Activated...cloning strategy, MoClo, which uses the Type IIS restriction enzymes BsaI and BpiI/BbsI to efficiently assemble...

  3. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...strengthened during memory formation and memory retrieval. In neuronal tracing experiments, brain tissue...Traditional tracing compounds either produce a colorimetric product or are fluorescent. While dyes usually...pressure injection or iontophoresis (applying small electric currents via inserted electrodes) may be applied...both antero- and retrograde labelling, synaptic restriction as well as controlled monosynaptic and polysynaptic...polysynaptic spreading. AAV vectors cover some of these attributes and their use has allowed for the visualization...recombinant AAV serotypes 1, 2, 5 and 8 in the rat nigrostriatal system. J Neurochem 109, 838–845. PubMed PMID...PMID: 19727139. Ugolini, G., Kuypers, H.G., and Strick, P.L. (1989). Transneuronal transfer of herpes ...

  4. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...identify. Improved Restriction Enzyme Handling - SnapGene’s software positions restriction enzyme annotations...paste functionality. Restriction Enzymes Here you'll find a table of restriction enzymes that cut the...previous mapping software and now includes Type IIS restriction enzymes that are commonly used for Golden Gate...clicking on column heads. Clicking on a particular restriction enzyme here also highlights it in the “Map” and...

  5. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...make efforts to keep it updated with new entries and update entries as research progresses, but you are invited...Additionally, the Phylogeny tool allows users to browse entries by the current phylogenetic nomenclature and discover... database, and all researchers are invited to contribute to it! The CasPEDIA Consortium will, of course... invited to reach out to suggest new entries or even new features to the tool. Resources and References...References References Adler, B., Trinidad, M., Bellieny-Rabelo, D., et al. (2023). CasPEDIA Database: A Functional...

  6. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...use restriction digest to screen your clones, but there are many other ways to choose restriction enzymes...blue-white screen. Image from Stefan Walkowski.   Restriction digest Another way to distinguish between colonies...inserts from those that do not is by using a restriction digest. The key is to be strategic about your...route, check out our protocol video on using restriction digests to analyze plasmids, which can help you... you visualize this process, or visit our restriction analysis blog post. Colony PCR Colony PCR can...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...coupled with heterotrimeric G proteins on the intracellular side of the cell membrane. Heterotrimeric G proteins...in signal transduction pathways and consist of a trimer of three subunits: alpha (Gα), beta (Gβ), and gamma...the GPCR allows for binding of a new inactive heterotrimeric G protein complex and subsequently another ...elucidate the role of 14 of the 16 non-visual heterotrimeric G proteins that can be activated downstream... alpha, 2 beta, and 4 gamma subunits of the heterotrimeric G protein complex that can be used to detect...

  8. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...construct for C. elegans gene knockdown Article contributed by Alyssa Cecchetelli  Listen to the C. elegans... labeling with TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo...compartments is via proteomics--the study of the distribution and interactions of proteins within these structures...small-molecule substrate to map the spatiotemporal distribution and interaction networks of specific proteins...Changing the way we detect viral outbreaks Article contributed by Izzy Mueller  Listen to the viral detection... reveals novel peptide ligand motifs Article contributed by Michael Lemieux  Listen to the SH3 domain ...

  9. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...from AAV Purification by Iodixanol Gradient Ultracentrifugation First, you’ll want to identify the reader...for AAV Purification by Iodixanol Gradient Ultracentrifugation. The reader may be, for example, a graduate...between a gradient tube before and after ultracentrifugation so that a scientist can extract virus from...interfaces between layers are blurred after centrifugation and the syringe needle is inserted bevel-up...profile photo of an iodixanol gradient after ultracentrifugation. The interfaces between layers are muddled...illustrates a gradient tube before and after ultracentrifugation. The viral supernatant migrates through increasingly...

  10. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...March 2015, Addgene reached the 500,000 plasmids distributed milestone! We were thrilled to see the impact...one plasmid deposited at Addgene that has been distributed more than 100 times.   “I really like this award... labs worldwide use our plasmids. I hope they contribute to great discoveries!” — Julien Béthune, Professor...Flame Award on Bluesky. In 2016, we also began distributing ready-to-use viral vectors from some of our ...supportive educational resources. By 2017, we had distributed 1,000 ready-to-use viral vector preps. Today,...citations of individual items in our repository, a tribute to how many discoveries have been made because ...

  11. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...translation by sequestering the RBS, but binding of the “trigger RNA” (in this case, biomarker RNA or 16S rRNA isolated...can also detect expression of toxin mRNA from Clostridium difficile. As mRNA expression cannot be identified...fluorescent markers to visualize localization and distribution of gut bacteria without fixation or preparation... count cell divisions. The technique, called distributed cell division counting (DCDC), relies on the ... for both in vitro and in vivo studies. Find distributed cell division counting plasmids here References...PMCID: PMC2752045. Myhrvold, Cameron, et al. "A distributed cell division counter reveals growth dynamics...

  12. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...your cloning strategy: Restriction enzyme digestion Be mindful of the restriction enzymes you chose. Make...This post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo...which technique you are going to adopt (i.e. restriction cloning, Gibson Assembly, Golden Gate etc.), ... fragments of the size you expect, check the restriction pattern of the enzymes you chose, and verify ...hopefully have some new strategies to tackle that tricky cloning project. Good luck with your experiment...

  13. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ... added level of confidence in the plasmids we distribute through our quality control processes. Every ... Addgene, we collect data on every plasmid we distribute through our deposit process, and then we carefully...functional mutations, antibiotic resistance, restriction sites used during cloning, and most importantly...sequencing reactions in parallel. We have a few tricks to simplify the process. First, nearly all of our...sequencing from the exact same stock that we store and distribute from. Of course, your culture should always be...sequencing reads? Tell us your favorite sequencing tricks in the comments section below.  Additional Resources...

  14. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... caps, compatible with your centrifugation speed, to cover the centrifuge bins. These have to be added...okay to add another level of protection! When centrifuging viruses (a necessary step during production)...significant risk for aerosol contamination. As most centrifugation has to be done outside of a biosafety cabinet... and removed in a biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra ... spinning down lentivirus, you’ll need to use centrifuge bin covers, which you should open in the biosafety...

  15. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...183 gene therapy trials (http://www.abedia.com/wiley/vectors.php). These clinical trials have been directed...This post was contributed by guest blogger Harshana S De Silva Feelixge. Gene therapy technologies ...gene therapies from conceptual design to clinical trials involve identifying a therapeutic gene, finding...most naturally occurring viruses used in clinical trials, wild type AAV is not associated with any pathogenicity...While basic research and other ongoing clinical trials support the utility of AAV as a broadly applicable...

  16. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...science has become increasingly restricted to academic and industrial labs, but recently there has been ...The following post was contributed by Derek Jacoby from Makerspace Victoria, CA Over the decades science...possible that we’ll find something interesting and contribute it back to the kittybiome project, but the primary...generally funded on a membership model - everyone contributes each month - with only occasional supplementation...would otherwise be discarded. For instance, the centrifuge in the picture had a broken controller and would...

  17. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...volume of lysate passed over the binding matrix could clog the matrix when performing filter based plasmid...DNA to verify your plasmid via sequencing or restriction digest. Chances are, you’ll even have DNA left...media You can achieve higher cell density using a nutrient-rich broth such as Terrific Broth, 2xYT, or a ...longer growth time. Note: Julian Taylor-Parker contributed to the writing of this post. Additional Resources...Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources Sigma-Aldrich Introduction...

  18. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...subjected to quality control by Addgene before being distributed to customers. Details about our production protocols...control are described below. AAV Production AAV distributed by Addgene has been produced either in-house ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a 0.45 µm polyethersulfone...in 10% polyethylene glycol (PEG) followed by centrifugation. Precipitated pellets containing viral particles...

  19. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...commercial lateral flow strips, similar to pregnancy tests. With lateral flow strips, the presence of your...determined by the number of bands present on the strip. This type of readout allows for nucleic acid detection...detection almost anywhere as lateral flow strips are easy to transport and work rapidly, providing reliable...platform to detect SARS-CoV-2 using a lateral flow strip format. They published this in Nature Biotechnology... validation studies.     Jennifer Tsang contributed to updating this article. References Abudayyeh...

  20. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...from 5,821 labs in 1,249 institutions across 55 countries.  Our first request was on September 8, 2004 (...pcDNA-p160MBP, if you're curious.) Since then, we've distributed 2,002,539 materials to 254,034 scientists in ...in 8,900 institutions across 111 countries. Just eight days into 2024, we've already shipped 2,078 items...scientists at 375 organizations in 21 different countries. It's safe to say we keep our shipping team busy...international community, we've partnered with distributors in China, Korea, Japan, India, and Brazil.  ...

  21. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...Glove trick Putting on lab gloves can sometimes be annoying. To make it easier, try this quick trick. Just...Addgene has distributed over 800,000 plasmids since 2004. In that time, our lab has gotten a lot experience...Just flip, and press! Lab tip #2: Tube flick trick When combining reagents in the lab, it is important...several times. This will help ensure ensure equal distribution. Lab tip #3: Barcode everything Use barcodes...

  22. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    Published
    Oct. 8, 2019, 12:33 p.m.
    ...publication and we have already collected over 100 entries spanning six different species and dozens of different...tool hM4D(Gi) driven by a GFAP promoter in rat. Contribute your AAV data Just like Addgene’s plasmid repository.... We are grateful to the scientists who have contributed data so far and we encourage you to submit data...the data hub website as a thank you for your contribution to open science.  Please note that the AAV Data... currently only accepting data for materials distributed by Addgene.  Future directions As the AAV Data...

  23. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration is the most common analytical...mScarlet-tubulin (Plasmid #85045). We often use restriction enzymes in the multiple cloning site for generating...Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al., 2001, Adjobo-Hermans...Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation in living cells." BMC...Devreotes. "Receptor-mediated activation of heterotrimeric G-proteins in living cells." Science 291.5512...

  24. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...genetically encoded fluorescent probe. Several factors contribute to the popularity of GFP including (i) fast and... mutation (A206K) is necessary to generate the strictly monomeric EGFP variant, mEGFP (Zacharias et al... bind the molecule of interest. For instance, pleckstrin homology domains can be used to detect specific...have successfully generated several functional heterotrimeric G-alpha subunit fusions that do not tolerate...Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation in living cells." BMC...

  25. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...screen using PCR and a restriction enzyme digest.   Fig. 3: Restriction enzyme screening for resistivity...of your experiment for a screening strategy. Restriction enzyme screening is sensitive to single base ...changes, but the edit must occur at an existing restriction enzyme recognition sequence or generate a new... but your edit doesn’t affect any endogenous restriction enzyme sites? Never fear! You can introduce a...silent mutation should be designed solely for restriction enzyme sensitivity or resistivity, and thus be...

  26. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...forward to in 2023 and beyond. Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids...and general-use antibodies, is available in both trial- and full-size, with assay-supported recommended...our website saw 2.2 million visitors from 231 countries. Guides and protocols were our most popular content... million page views from visitors in over 200 countries. We, like everyone around the world, are learning...research community through sharing science and distributing high-quality materials and information. As a...

  27. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...When performing restriction digests on plasmids at Addgene, we sometimes observe something odd in our...various multimeric forms (e.g. concatemers, dimers, trimers, tetramers). Since then, multimerization has been...themselves cannot be used to detect multimers, since restriction sites in multimers usually repeat in tandem along...DNA without changing the size. As a result, a restriction digest of multimers will produce the same fragment...sample, you could try linearizing the plasmid via restriction digestion and then run the linearized plasmid...eBookAddgene's Molecular Biology ReferenceAddgene's Restriction Digest Protocol More resources on the Addgene...

  28. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...This post was contributed by guest bloggers and IMP organizers Rosario F. Godino, Rocío López-Diego, ...youth, our future, around the world.   In many countries of the world, professional internationalization.... Brain drain is a serious problem in several countries, however programs like IMP, bring closer than ...from talented professionals to their original countries to help build, together with the universities,...pre-program self-assesment. Achievements directly attributable to participation in the program included improvements... will also initiate mentoring pilots in other countries. In 2016, we expect the program to comprise over...

  29. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...nights, game nights, and trips to eat out together. “We also have a lab day trip each year and as we do ... the lab receives send-off via a gift, card, and trip to the pub. Dr. Ellis adds “we probably don’t celebrate...lot of yeast research, a few of those have been trips to breweries who happily exchange our expertise ...company outside of the lab by sharing meals, going on trips, and going to New Hampshire in the summer for the...

  30. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...through a distributor?  Yes! Packaged on Request service will be available through our distributor partners...of AAV plasmids will have technical or legal restrictions that make them ineligible for use in this service...international; varies widely by country and/or distributor.)  You should plan to receive your Packaged on...is launched, you’ll be able to work with the distributor to place a request and order if approved.  I ...

  31. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...on chemical (CaCl2, cationic liposome) or electrical (electric current forming membrane pores) transfection...virus. Virus purification can be a tricky process. You have to centrifuge the culture supernatant in order...The ability to infect non-dividing cells is not restricted to in vitro cell culture as lentivirus-derived...transducing non-adherent cells you might need to centrifuge your cells with the virus in order to aid viral...

  32. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...Restriction enzyme cloning is the workhorse of molecular cloning; however, one of its biggest limitations...that sequence modifications can only be made at restriction enzyme cut sites. The lambda red system is an... of DNA within E. coli and is independent of restriction sites. The lambda red system is derived from ...means cloning occurs in vivo, as compared to restriction enzyme cloning where the genetic changes occur...sections, key steps that differ from traditional restriction enzyme cloning will be explained in greater detail...screen for positive clones in most cases, and restriction enzyme digest can be used to screen plasmids ...

  33. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...This post was contributed by Kurt Swanson a structural biologist and protein engineer currently working...R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients...mouse models. As this biologic moves into clinical trials, there is new HOPE for AJ. But, as we in the scientific...fight against FOP in two ways. First, Addgene is distributing ACVR1 Plasmids from a variety of research labs...will be hosting the AJ’s Boston League team (Contributing many new heroes to the team in the process) ...

  34. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... a team to get a lot of stuff done.  Factors contributing to job satisfaction Being a good manager is ...mostly happy at work, they will work harder and contribute more. Assuming basic needs are being met (i.e...pay and benefits to live on), the factors that contribute to job satisfaction are a bit surprising. There...overwhelmingly agree that these are the factors that most contribute to job satisfaction: A feeling of being appreciated...organization and feel the importance of their contributions. Flexible work schedule Flexibility to manage...

  35. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...shut-down and the subsequent fluctuations in distribution. I don’t have space in this blog to call ...scientific community.  With the dramatic reduction in distribution, we had to figure out a way to maintain our ...operations until our normal work resumed. Addgene distributes and fulfills requests for scientists doing almost...especially proud to support Addgene now, when their distribution of plasmids for COVID-19 research is accelerating... cloning grade DNA service, and viral vector distribution is recovering from the pandemic-related slowdown...

  36. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...Human whole genome sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial steps...labeling using recombinase competition Article contributed by Aliyah Weinstein Sparse Predictive Activity...reporter for calcineurin activity in yeast Article contributed by Leah Schwiesow Calcineurin is a protein phosphatase...protein fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded...single molecule-fluorescence assay to monitor restriction enzyme digestion of single molecules in real-...

  37. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... This post was contributed by guest blogger James D. Fessenden, an Assistant Professor at Brigham and... site when used in conjunction with Cy dye-nitrilotriacetic acid (NTA) conjugates (Kapanidis, Ebright,...50-69. Royal Society of Chemistry. 3. Ermolenko, Dmitri N., et al. "Observation of intersubunit movement...-mediated fluorescent labeling with (Ni2+: nitrilotriacetic acid) n-fluorochrome conjugates." Journal ...

  38. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...immune response from the body. Several factors contribute to the immunogenicity of a drug, including the... body recognizes a drug as a foreign antigen, triggering a humoral immune response. ADAs can inactivate...one of the more common open-access tools, while EpiMatrix is a commercially available algorithm to evaluate...in silico assessments, as well as analyze the contributing factors that in silico prediction algorithms...monocytes. PBMCs can be further differentiated into dentritic cells or macrophages through cytokine induction...

  39. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds,...versatile offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA library, and...processes we use to ensure high-quality library distribution. You can also use these tips as a starting point... scaled to 1, to create a “cumulative wealth distribution” curve, or Lorenz curve. By calculating the ...gain a sense of the uniformity of the library distribution, with an AUC of 0.5 representing perfect uniformity...

  40. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ... is a silica matrix fixed in a column and you flow lysate and buffers through the matrix, usually by centrifugation... can inhibit important reactions like PCR or restriction digests. Transfection Grade DNA (high quality... centrifugation or vacuum. In a bead-based approach, you mix specially coated magnetic beads into your...constructs – Large plasmids, cosmids, or BACs, can be tricky to purify due to their size. Some manufacturers...a good option for you, some manufacturers offer trial or sample sizes of their kits, which you can use...

  41. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...presentation, starvation or the use of caloric restriction mimetics trigger autophagy and, in a mouse model led ...deposit and distribution service! References: 1. Pietrocola, Federico, et al. "Caloric restriction mimetics...from a state of chronic hepatitis caused by hypernutrition and alcohol consumption. Chronic inflammation...

  42. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...Light-mediated block of synaptic transmission Article contributed by Klaus Wanisch  Listen to the optogenetics ...imaging of microbes in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter...and create ultrasound contrast. At a very low volumetric density and with a resolution of less than 100...mechanisms of miRNA-mediated gene regulation Article contributed by Michael Lemieux  Listen to the miRNA reporter...vivo using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast...

  43. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...This post was contributed by Mary Tamer from BioBuilder, an innovative nonprofit bringing the study of... new for themselves and when they effectively contribute as part of a team.” As a seasoned educator, Kuldell...almost every state in the U.S. and in more than 30 countries around the world. Snapshots of the BioBuilder ...open-access curriculum begins with students reading comic strips and watching animations that present a specific... lab materials and reagents such as droppers, centrifuge tubes, circuit parts, and lab-strains of bacteria...

  44. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...He has recently developed a third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3...to identify cell populations in the brain that contribute to the development of behavioral signs of opioid...connectivity patterns of these cells and the functional contribution to a variety of behaviors related to addiction...expressed in a mammalian cell line engineered to be electrically excitable. The mutants are co-expressed with...membrane voltage,” says Tian. Figure 5: Citrine-tagged voltage indicator expressed in mouse cortex...

  45. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...collaborative group, she also joined early on and contributed her expertise in developing reproducibility workshops...Joanne Kamens, Addgene also had the opportunity to contribute to this open science collaboration. The first...workshop at the conference, but because each contributor made the materials accessible, anyone could present...present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing and were... upcoming conference or you’d like to join or contribute to this collaboration, please check out the official...

  46. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...worry about and that is something to recognize! Distributing plasmids, viral service, and more For the ...about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% every year...since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere can access plasmids... it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for more offerings....Addgene. There’s so much potential for service and contribution! Leadership On an organizational level, ...

  47. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...The post was contributed by guest blogger Londa Schiebinger, PhD, Hinds Professor of History of Science...the US Women’s Health Initiative Hormone Therapy Trial, for example, found that the study saved lives. ...was $35.2 billion (or $140 per $1 invested in the trial). The goal of gendered innovations It is crucially...research is minimal compared to that of human clinical trials. And, as Janine Clayton points out, late-stage ...because sex was not analyzed) may harm humans or contribute to failures in drug development – costs far greater...

  48. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin columns...producing and using lentivirus and offer some tips and tricks for overcoming these hurdles. Talking 'bout my...relatively inexpensive and does not require an ultracentrifuge, PEG will precipitate all proteins in a solution...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

  49. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...they are transferrable across most, if not all, industries and work environments. This is why they are called... bathroom mirror pep talk, forgotten and then retrieved your lucky socks, persevered through a nerve wracking...potential teammates want to be assured that you will contribute openness, clarity, approachability, and professionalism...interview, check out this article from The Muse. Kai Strickland is a former Customer Support and Operations Manager... Kai Strickland...
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