We narrowed to 123 results for: 11

1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   Published

   Sept. 26, 2024, 1:15 p.m.

   .... Nat Methods. 2007 Mar;4(3):251-6. Epub 2007 Feb 11. PubMed. SLIC: a method for sequence- and ligation-independent...

2. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed Central PMCID...

3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 ...

4. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...to create any program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the moon...

5. CRISPR 101: Non-Homologous End Joining

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   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ...Genome Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364-19 More CRISPR...

6. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

   Type

   Blog Post

   Published

   Sept. 28, 2020, noon

   ...Mycobacterial species, Sarah Fortune’s lab screened 11 other Cas9 orthologs to identify a more efficient... Phage-Delivered CRISPR-Cas3 Antimicrobials. mBio 11: . https://doi.org/10.1128/mbio.00019-20 Singh AK...

7. Optogenetics + CRISPR, Using Light to Control Genome Editing

   Type

   Blog Post

   Published

   Sept. 3, 2020, 12:15 p.m.

   ...expression of human IL1RN, the researchers saw an 11-fold increase in mRNA levels after 2 hr and a 400...control of endogenous gene activation. Nat Chem Biol 11:198–200 . https://doi.org/10.1038/nchembio.1753 Richter...

8. Delivery Methods for Generating iPSCs

   Type

   Blog Post

   Published

   April 17, 2018, 1:37 p.m.

   ...generating iPSCs by plasmid transfection where 2 of 11 clones tested had plasmid integration. Another challenge...Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no. 10 (2016). PubMed PMID: 27764162. PubMed Central...

9. 22 Hot Plasmid Technologies from 2014

   Type

   Blog Post

   Published

   Jan. 6, 2015, 5:21 p.m.

   ...12005-15. Hernandez et al., Autophagy 2013 Nov 1;9(11):1852-61. A quicker method for protein inactivation...variants. Klapoetke et al., Nat Methods 2014 Mar 11;(3):338-46. For more information and descriptions....3179. Martell et al., Nat Biotechnol 2012 Nov;30(11):1143-8.  A new tool for CRISPR gRNA validation...

10. Viral Vectors 101: Systemic Capsids

    Type

    Blog Post

    Published

    Oct. 12, 2023, 1:15 p.m.

    ...non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419-x Deverman...cellular receptor for AAV-PHP.B capsids. PloS One, 14(11), e0225206. https://doi.org/10.1371/journal.pone.0225206...prioritization for cell type-specific enhancer design. eLife,11, e69571. https://doi.org/10.7554/eLife.69571 Liguore...Primates Compared with Mice. Molecular Therapy, 27(11), 2018–2037. https://doi.org/10.1016/j.ymthe.2019.07.017...

11. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    Published

    June 16, 2022, 1:15 p.m.

    ...-page genre fiction novella as it does to read an 11-page Cell paper - and sometimes, it might actually...

12. GPCRs: How Do They Work and How Do We Study Them?

    Type

    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ...subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11, and Gα12/13. Gβ and Gγ are closely associated and...

13. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...other labs discovered that the in vitro functions of 11 different Arg-mutant enzymes could be rescued by ...

14. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ...PMID: 26280411. PubMed Central PMCID: PMC4785103. 11. Wong, Alan SL, et al. "Multiplexed barcoded CRISPR-Cas9...

15. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ...EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants retained the on-target activity...

16. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...Engler C, Kandzia R, Marillonnet S. PLoS One 2008;3(11):e3647. doi: 10.1371/journal.pone.0003647. Epub 2008...

17. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...of the chimeric and fully albino mice ranging from 11% to 100%, a level similar to other approaches.  Nanoblades...

18. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...Dujon, B. Yeast evolutionary genomics. Nat Rev Genet 11, 512-524. (2010). PubMed PMID: 20559329. 8. Reeves...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...genome editing with engineered nucleases. Nat Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev...

20. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ...in science and scientists (as discussed in Chapter 11)? Even if some of the problems are addressed in a...