We narrowed to 120 results for: ALC

1. Fueled by Coffee at #SfN14

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   Blog Post

   Published

   Nov. 18, 2014, 7:15 p.m.

   ... global coffee supply… My back-of-the-napkin calculations put the daily consumption here at ~ 60,000 cups...

2. Fluorescent Proteins 101: History of Fluorescent Proteins

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   Blog Post

   Published

   Aug. 7, 2017, 1:58 p.m.

   ...aequorin, gives off a faint blue light when it binds calcium ions, and the second, which we now call GFP, absorbs...

3. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

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   Blog Post

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   Jan. 8, 2014, 3:10 p.m.

   ...GCaMP5 and GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year ...

4. Editor's Choice, September 2016

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   Blog Post

   Published

   Oct. 7, 2016, 4 p.m.

   ... can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards...

5. Mapping the 4D nucleome with CRISPR/Cas9

   Type

   Blog Post

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   Aug. 11, 2015, 2:30 p.m.

   ...physical map distances of 75 and 2 Mbp, with the calculated fluorescent distances correlating with the previously...

6. 28 Hot Plasmid Technologies from 2015

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   Blog Post

   Published

   Dec. 23, 2015, 3:30 p.m.

   ...fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation...multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming soon!). Takai, et al...

7. Hot Plasmids and Viral Preps - July 2021

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   Blog Post

   Published

   July 27, 2021, 1:15 p.m.

   ...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments...

8. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   Blog Post

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   Feb. 13, 2018, 2:51 p.m.

   ...targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity...

9. Sensing Neuronal Dopamine

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   Blog Post

   Published

   July 30, 2019, 12:59 p.m.

   ... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...

10. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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    Blog Post

    Published

    July 16, 2019, 12:59 p.m.

    .... The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement that results...

11. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    Blog Post

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    Jan. 15, 2015, 1:50 p.m.

    ...genomes, such as the malaria-causing Plasmodium falciparum. Throughout these talks, I oscillated between...

12. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    Published

    Aug. 31, 2023, 1 p.m.

    ... previous implementation of optogenetic tools, calcium sensors, and other common neuroscience tools into...

13. Plasmid Cloning by PCR

    Type

    Blog Post

    Published

    March 29, 2016, 2:30 p.m.

    ...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

14. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type

    Blog Post

    Published

    March 4, 2014, 4:49 p.m.

    ...modulate electricity. If you could selectively let in calcium or some other specific ion that would be interesting...

15. Visualizing Translation at the Single Molecule Level

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    Blog Post

    Published

    Aug. 1, 2017, 1:15 p.m.

    ...inhibitor that stalls ribosomes after initiation, to calculate a ribosome translocation rate of ~3.5 codons/second...

16. Addgene Depositors Get More Citations

    Type

    Blog Post

    Published

    April 20, 2017, 2:30 p.m.

    ... of publication. Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example...

17. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...feature filter spectra and collection efficiency calculations.  With ~80 FP spectra (and rapidly growing, ...

18. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type

    Blog Post

    Published

    Oct. 26, 2017, 1:59 p.m.

    ...wealth distribution” curve, or Lorenz curve. By calculating the area under the curve (AUC), one can gain ...

19. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    Published

    June 4, 2019, 1:02 p.m.

    ... of serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured...

20. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type

    Blog Post

    Published

    Feb. 13, 2024, 2:15 p.m.

    ...algorithms are trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al...