We narrowed to 117 results for: ALC

1. Editor's Choice, September 2016

   Type

   Blog Post

   Published

   Oct. 7, 2016, 4 p.m.

   ... can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember the hazards...

2. Mapping the 4D nucleome with CRISPR/Cas9

   Type

   Blog Post

   Published

   Aug. 11, 2015, 2:30 p.m.

   ...physical map distances of 75 and 2 Mbp, with the calculated fluorescent distances correlating with the previously...

3. 28 Hot Plasmid Technologies from 2015

   Type

   Blog Post

   Published

   Dec. 23, 2015, 3:30 p.m.

   ...fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation...multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming soon!). Takai, et al...

4. Sensing Neuronal Dopamine

   Type

   Blog Post

   Published

   July 30, 2019, 12:59 p.m.

   ... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...

5. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

   Type

   Blog Post

   Published

   July 16, 2019, 12:59 p.m.

   .... The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement that results...

6. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

   Type

   Blog Post

   Published

   Feb. 13, 2018, 2:51 p.m.

   ...targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity...

7. Hot Plasmids and Viral Preps - July 2021

   Type

   Blog Post

   Published

   July 27, 2021, 1:15 p.m.

   ...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments...

8. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

   Type

   Blog Post

   Published

   Jan. 15, 2015, 1:50 p.m.

   ...genomes, such as the malaria-causing Plasmodium falciparum. Throughout these talks, I oscillated between...

9. Multifunctional Neural Fibers: Modulation from Gut to Brain

   Type

   Blog Post

   Published

   Aug. 31, 2023, 1 p.m.

   ... previous implementation of optogenetic tools, calcium sensors, and other common neuroscience tools into...

10. Plasmid Cloning by PCR

    Type

    Blog Post

    Published

    March 29, 2016, 2:30 p.m.

    ...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

11. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type

    Blog Post

    Published

    March 4, 2014, 4:49 p.m.

    ...modulate electricity. If you could selectively let in calcium or some other specific ion that would be interesting...

12. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    Published

    June 4, 2019, 1:02 p.m.

    ... of serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured...

13. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...feature filter spectra and collection efficiency calculations.  With ~80 FP spectra (and rapidly growing, ...

14. Visualizing Translation at the Single Molecule Level

    Type

    Blog Post

    Published

    Aug. 1, 2017, 1:15 p.m.

    ...inhibitor that stalls ribosomes after initiation, to calculate a ribosome translocation rate of ~3.5 codons/second...

15. Addgene Depositors Get More Citations

    Type

    Blog Post

    Published

    April 20, 2017, 2:30 p.m.

    ... of publication. Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example...

16. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type

    Blog Post

    Published

    Feb. 13, 2024, 2:15 p.m.

    ...algorithms are trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al...

17. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type

    Blog Post

    Published

    Oct. 26, 2017, 1:59 p.m.

    ...wealth distribution” curve, or Lorenz curve. By calculating the area under the curve (AUC), one can gain ...

18. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type

    Blog Post

    Published

    June 1, 2017, 2:30 p.m.

    ...) respectively. The theoretical brightness is calculated by multiplying EC by QY (EC*QY, sometimes normalized...

19. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...wild type target in each sample can be quickly calculated (details in [2]) and can be used to quantify ...

20. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...site. A specificity ratio can be measured by calculating the on-target activity divided by the off-target...