...recombinant antibody. Researchers first sequenced the variable domains of Myc1-9E10 in the late 1990s (Fuchs,...anti-Myc [9E10] Fab fragments and single-chain variable fragments.  Abi, on the other hand, might...mentioned above basically involve taking the Myc1-9E10 variable regions and sticking them onto different scaffolds...Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence...

... and this nuclease-null or “dead” Cas9 (dCas9) variant can then be fused to effector domains such as transcriptional...limitations to a system that relies on two distinct Cas9 variants—nuclease-competent or nuclease-null—in order to...with both the nuclease-positive and nuclease-null variants of Cas9 along with the necessary gRNAs, but it...control which gRNA becomes complexed with which variant of Cas9. To circumvent this problem, previous studies...

...such as malaria and dengue for many years. Since more than a thousand children died of malaria today, ... tropical mosquito vectors of diseases such as malaria and dengue are currently being performed in regions... cas9 gene). An immunizing reversal drive is a variant that also spreads through the wild population and...

...FPs just keeps getting larger with hundreds of variants available. Read on to familiarize yourself with...emission wavelenght range).  By mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP...YFP) were derived. For a breakdown of GFP, it's variants, and their relevant mutations, check out Marcy's...with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) ...

... Having incorporated a discovery-based lab on planarian regeneration into one of my own courses, I was...required for stem and progenitor cell maintenance in planarians in Developmental Biology (Kimball et al., 2020...undergraduates conducting NIH- and NSF-funded research on planarian flatworms both in and out of the classroom.   ...required for stem and progenitor cell maintenance in planarians. Developmental Biology. 457:119-127. Knutson,...

...vivo. Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous...PE6d are optimized for dual-AAV systems. These variants use different RT enzymes and performed large edits...such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...edit.   Figure 1: Choosing the best PE6 variant for your application. Image reused form Doman and...

... et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP after being targeted...changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus...the test by infecting HEK-293 cells with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for...the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base editing event...

...for synaptic glutamate response. They generated variants optimized for different applications (v82 has ... and powerful high-throughput screen to select variants based on performance metrics specifically for ...BeadScan platform for screening candidate biosensor variants for performance under two-photon fluorescence ...heat with B-gTEMP  Measuring local temperature variations and heat transfer at the subcellular level requires...

...response recorded would be due to natural biological variation. This information is critical for correctly interpreting...of the same sample and are included to assess variability in the experimental protocol. In the experiment...results vary considerably, then the high degree of variability in the process could make it difficult to obtain...assess naturally occurring random biological variability. If one were studying the effects of tau kinase...

...any optogenetic experiment. There are numerous variants for the different types of opsins available and...cell-type specificity, the kinetics of the opsin variant, and other particularities that best suit your ...these reviews for a discussion of the different variants [1, 2, 4]. And, of course, the best way to learn...learn about the many different opsin variants and how they can be used is to consult the many primary research...

...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

...Pasteur, Paris) that changed genome editing in the malaria community for the better (Nat. Biotechnol., 2014...Plasmodium falciparum (the parasite that causes malaria). I attribute their success to technical expertise...the software piñata has been filled with slight variations on a theme without many resources to review them...Ghorbal, Mehdi, et al. "Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9...

...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient...

...photoactivatable repressor (dCas9). The activity of all variants was reversible and repeatable. Since these initial...CRISPR–Cas9 activity switching via a novel optogenetic variant of AcrIIA4 (CASANOVA). As shown in the figure below...Publication System nickname Photoactivatable moiety Cas9 variatn CRISPR protein application Split Systems / Light-induced...Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Research 44: . https://doi.org/...

...developing transgenic mice that express camelid variable domains (aka nanomice), which gives researchers...modifications made to generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice... camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586...

... sensor. This sensor was engineered from a GFP variant and a DA receptor such that GFP fluoresces when...when the receptor is bound by DA. Two variants of the sensor, dLight1.1 and dLight1.2, exhibit high (submicromolar...GRABDA sensor based on a similar strategy: a GFP variant bound to a DA receptor, such that the GFP fluoresces...

...AAV1 Biosensor Marianne Fyhn New viral prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

...Polyclonal antibodies produced in animals may be variable among animals and bleed dates. The development...as other affinity reagents such as single-chain variable fragment (scFvs) and nanobodies. Addgene will ...Hybridomas frequently express additional functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080...

... are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...In mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best...measurements. Here, EGFP outperforms the improved folding variants, presumably due to the lower growth temperature...

...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...subclones within a single well reduced well-to-well variability, while the readout was able to be deconvoluted...