...of your prep. Many plasmid prep kits recommend performing an extra wash step to remove endonucleases (see...the binding matrix could clog the matrix when performing filter based plasmid prepping. Be sure to stay...yield as these factors are generally, but not perfectly, correlated. Typical growth times for high copy... to Do a Bacterial Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources...

...tested in yeast5. Ecological confinement involves performing experiments in a geographic area where escaped...such as malaria and dengue are currently being performed in regions that don't have resident populations...very few gene drive experiments that can't be performed with two or more confinement methods. Since molecular...scientists could not be trusted to handle such a powerful technology responsibly. An accidental release ...

... has refined our use of the BLAST web browser interface to be as efficient as possible. If you find yourself...sequences is probably the fastest BLAST search to perform and will save you time compared to other types ...parameter, as this will affect the amount of time to perform the search as well as the overall alignment results...correct!]  Since Sanger sequencing reactions are imperfect and sequence near the beginning or end of a reaction...

...LwaCas13a, which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In ...resulted in 15% C-to-U editing activity. They then performed sixteen rounds of directed evolution across the... cytidine deaminase activity. While RESCUE can perform C-to-U edits, the authors note that it retains ...whole-transcriptome RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A...

...company benefits from their policy! Perfectionism When we reach for perfection, our work stalls. We become so...seems impossible to start at all. This is why perfectionists tend to also be procrastinators.  If you fall...timeframe. Tell yourself they don’t have to be perfect or even good. The goal is simply to throw out ideas...

...you may have been a little surprised at how this powerful tool is in such a small package. But don’t let... be skipped or whether selecting colonies and performing Sanger sequencing will suffice…and the answer...mostlibrary requests specifically to allow scientists to perform functional titering and optimize transduction efficiency...finally!) ready to use your transduced cells to perform your pooled library screen…but that’s a blog post...

...inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives." Proceedings of the...However, it can be difficult to compare their performance, as most translation initiation sequences are...translation system. The gene set was carefully chosen to perform statistically relevant benchmarking of cell-free...size and expression level can be conveniently performed through the N-terminal eGFP tag carried on all...

...Cryo-EM samples can be damaged at the air-water interface (AWI) during the freezing process, resulting in...When proteins (blue) interact with the air-water interface (AWI) before vitrification, they can be completely... Small LEA proteins as an effective air-water interface protectant for fragile samples during cryo-EM ...PASSIGE efficiency (Pandey et al., 2024). The best-performing variant yielded an average integration efficiency...

...multiplexing to multiscaling NIR-shifted spectrum is perfect for use in spectral multiplexing (Shcherbakova ...the monomeric state of a probe leads to less interference with the oligomeric state of a fusion partner...mIFP6. Learn more about the characteristics, performance, and applications of near-infrared calcium indicators...state-of-the-art NIR fluorescent probes and in choosing the perfect one for your experiment!   Thank you to our guest...

...constructs), in functional analysis of proteins (to perform alanine scanning mutagenesis or targeted substitution...). Such non-complementary bases at the 3’-end interfere with plasmid reconstitution, and consequently ... of primer duplications), final validation is performed by sequencing functional regions of the plasmid... at extremely low frequency), and these could interfere with the function of the resulting construct. ...

...several brain regions where other serotypes may perform better (3). Brain Region  Optimal Serotype ...Cycles Of course, one cannot truly predict the perfect titer, but one of the main recommendations when...expressed that anatomical assessments can be easily performed too. While I implied that axoplasmic transport...vectors that will have substantially improved performance profiles and should be suitable for genome editing...

...selective transgene expression. While this is a powerful experimental strategy, one of the most common ...is to know how the AAV vector you intend to use performs in your system. This includes a thorough testing...experiments requires a full understanding of vector performance in your system. It is very rare for a methods...known and unknown factors that affect vector performance in each and every system and cell type. Therefore...

...scholar/student: visiting from another university to perform research   Why should I try research? Personally...research, and if that’s where you’re at too, it’s a perfectly great place to begin. Here’s my advice: Reflect...Reflect on your personal interests While it’s perfectly fine to be unsure, it helps to have a vague sense ...about yourself and the world around you, it’s perfectly normal to realize that you have new interests ...

...allow scientists to use CRISPR-Cas technology to perform genome-wide knockout screens. These vectors and...utilized to create recombination template vectors, to perform multiple site mutagenesis and create complex fusion... and Chrimson, which have enabled the group to perform independent two-color optical excitation of neurons...subdivisa (CsChR), Chloromonas oogama (CoChR), and Scherffelia dubia (SdChR). ShChR, nicknamed Chronos, is blue...display. APEX2 has been shown to exhibit superior performance over APEX for both EM imaging and proximity-dependent...

...AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform for... to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition...

...We perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We...aliquoted and stored at -80oC until we are ready to perform transformations. A typical 200mL bacterial culture... of the large number of transformations being performed at Addgene, this adds up to a significant time...

...how we use next generation sequencing results to perform quality control on deposited plasmids. We’ve also...improved feature detection with an easy to use interface. We regularly use Snapgene for our quality control...region is present in the plasmid, we might need to perform a restriction digest to confirm the size of the...

...various conditions. However, this system is far from perfect. For example, If your fusion protein is expressed...GFP aggregation, which they reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1...What can you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper...

... While restriction cloning is still routinely performed by many labs, a variety of other cloning techniques...transformation. After transforming cells, you should perform colony PCR with vector specific primers to confirm...Resources on the Addgene Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your...

...react by performing a distinct C-bend to escape possible danger (see figure). Larvae also perform spontaneous...effects on behavior. The system is an especially powerful tool for in vivo behavioral studies because it...