We narrowed to 82 results for: GRI;
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TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....horribly wrong. It brought the whole field to a grinding stop and only now, 2 decades later, is it finally...
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Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....impact the level of permeabilization and membrane integrity (Fig. 2). For example, saponin interacts with ... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....58.4 (2015): 644-659. PubMed PMID: 26000849. 2. Grimm, Jonathan B., et al. "A general method to improve... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....At N-terminus, promotes folding and structural integrity; cleavable. Not great for purification; too cleavable... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m.... questions Now let's get into some of the nitty gritty and answer the questions those of us at Addgene... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....results are analyzed to confirm the identity and integrity of the viral genome and the absence of unexpected... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....Michael D. L. Johnson, Tiffany Lowe-Power, Eric Griffis, Janet Iwasa, Shawnna Buttery, Kat Milligan-Myhre... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m.... your Gene of Interest! Be sure to verify the integrity of your expression clone via sequencing or restriction... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m.... S, Wiedtke E, Niopek D, Mueller A-K, Börner K, Grimm D (2014) CRISPR/Cas9-mediated genome engineering... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....Under the co-mentorship Blossom Damania and Jack Griffith, her research focuses on the replication machinery... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog PostPublishedNov. 16, 2021, 2:15 p.m....smaller steps, but the aim is to preserve sample integrity and fluorescence and to capture your results. ... -
March for Science
TypeBlog PostPublishedApril 21, 2017, 2:30 p.m.... EPA programs: draft budget Science Magazine: A grim budget day for U.S. science: analysis and reaction... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m....cell-free expression systems, and tested for product integrity, expression level, and aggregation propensity ... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m....inflammation and necrosis compared to unedited animals. In grip strength, specific force, and other muscle tests... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....personal standing – including undermining personal integrity, destructive innuendo and sarcasm, persistent ... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....get you hung up. Here we will dive into the nitty gritty details of comparison statistical tests – when ... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....18614669. Belzung, Catherine, Marc Turiault, and Guy Griebel. "Optogenetics to study the circuits of fear-and... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....: 8627803. PubMed Central PMCID: PMC190186. 9. Grieger, Joshua C., and Richard J. Samulski. "Packaging... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog PostPublishedJune 4, 2024, 1:15 p.m.... Doudna, 2017). Within an interior cavity, Cas9 grips nucleotides 1-10 of the guide RNA, prearranged for... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m....22022444. PubMed Central PMCID: PMC3192723. 21. Grigoriadis, A.E., et al., Directed differentiation of hematopoietic...