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We narrowed to 388 results for: HAL;

Showing: 61 - 80 of 388 results

  1. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...cycle of one step. Challenges in the LEGO science video process One major challenge when we started was...will bring people together and make these daily challenges a little easier to overcome. We want to encourage...

  2. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...team called Running for Rare Disease that runs on behalf of the National Organization for Rare Disorders...HOPE; a fitting superpower for those running on behalf of rare disease sufferers like AJ. As the Blue ... First-In-Man studies. Kurt has been running on behalf of AJ Gonzales, a young boy suffering from FOP,...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...translation inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is necessary ...translation is known to be important in development, and Halstead et al. used TRICK to examine translation of Drosophila...Find ribosome profiling plasmids at Addgene. 2. Halstead, James M., et al. “Translation. An RNA biosensor...

  4. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...two genes by one Gal4 driver will produce roughly half as much protein as overexpression of each gene individually...384–391 (2004). PubMed PMID: 15262411. 4. T. D. Southall, D. A. Elliott, A. H. Brand, The GAL4 System :...Marka, J. C. Kagan, Environmental Stress Causes Lethal Neuro-Trauma during Asymptomatic Viral Infections...

  5. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...sources of information if those sources affirm or challenge their beliefs. I asked participants to read short...read an abstract for a study with findings that challenged their beliefs, they were much more likely to ...a phenomenon called “motivated reasoning,” the catchall term for a range of neat little tricks our minds...

  6. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...about 65,000 messages and dealing with email can be half of my day sometimes.” He points out that the copious...the influence from his two PhD advisers who were “chalk-and-cheese”. While one was an established Professor....” Take home message Starting a lab can be a challenging process. Each PI emphasizes the importance of...

  8. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...components of EvolvR and its potential applications. Challenges of existing mutagenesis methods Several continuous...drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...PMID: 30253789. PubMed Central PMCID: PMC6154929. Halperin, Shakked O., et al. "CRISPR-guided DNA polymerases...

  9. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...movement is that it provides interesting and unique challenges for intellectual property lawyers. While there...be, an interesting legal and professional challenge.Paratore: The promise there is the ability share the...St. Elizabeth’s Hospital and was associated with Hale & Dorr and Crowell & Morning. Karen was a founding...

  10. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...computation work comes with new sets of challenges. One challenge with computational analyses is to keep... are doing what you are doing. Another common challenge is to keep track of what works, and what does ...

  11. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...year (12% in 2018) since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere...happening. The Addgene team will have some fun challenges this year and I’m excited about this, but definitely...talent pipeline. Turnover at Addgene is about half of what is standard in most sectors. The Addgenies...

  12. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...quantification of enhancer activity have remained challenging due to the variable availability of enhancer ...pTALYM3, pTALYM4 Boris Greber pTAL7a, pTAL7b Michal Zurovec pBlue-TAL Nathan Lawson pJDS Series ...degredation is the speed at which it works--typical half lives of AID-tagged proteins after the introduction...studying essential proteins. Until recently, it was challenging to apply the AID-tagging system to essential ...expressing and purifying human kinases remains challenging. Small molecule kinase inhibitors usually target...

  13. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...available planarians: Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis....differ. For example, Girardia sp. and Dugesia dorotocephalacan can be fed dsRNA-expressing E. coli that are...

  14. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which affect hemoglobin. Most ... enzymes target several strains of E. coli and lethally chop their genomes into many, many pieces. Locus...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...

  15. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ... cargo (DNA or RNA) to target cells has been challenging. A variety of vector delivery technologies have...treatment appears to be both safe and effective. Challenges for AAV gene therapy Although AAV-based gene ...promising, scientists are working to overcome key challenges to their efficacy:  Pre-existing immunity: Many...

  16. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...which creates a challenge when studying them since their complete knockdown is lethal. Incomplete or titratable... to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into diverse bacteria...

  17. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...infected host cell is eventually lysed. Cell lysis halts protein production, but there are non-lytic insect... however, are expensive and very technically challenging to use. Alyssa D. Cecchetelli is a Scientist... expression in Escherichia coli: advances and challenges Production of recombinant proteins in plant root...

  18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...the tag for each insertion. Also, it remains challenging to screen for the genetically modified strains...fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc...small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed a polymerase...

  19. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...be split into two halves, which are non-fluorescent on their own. If the two halves are in close proximity...determine the proximity of two proteins fused to the halves of the split FP. This technique is also is also...

  20. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ... peripheral nervous system presents a special challenge to scientists. What works well in one cell or ...vectors in neuroscience Let’s consider my first challenge when I started working with AAV vectors. I wanted...Adeno-Associated Viral Vector-Mediated Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https:/...
Showing: 61 - 80 of 388 results