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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 262 results for: INO

Showing: 61 - 80 of 262 results

  1. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ... is the nucleobase component of the nucleoside inosine. Created with BioRender.com. How does base ...adenine base editor that would convert adenosine to inosine, resulting in an A to G change (Gaudelli et al....non-G conversion at target loci. The removal of inosine from DNA is likely infrequent, thus preventing ...

  3. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...” diagrams, which show the interactions between amino acids in an approximate, two-dimensional map. The...topology, then iteratively generated 3D models and amino acid sequences that got closer and closer to achieving...

  4. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...successful streak plate, first the bacteria are inoculated over a quadrant of an agar plate. Then, a stick...Plate Challenge, Westenberg’s course includes Winogradsky columns and characterizing the microbes from ...

  5. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers...Drosophila melanogaster." Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169....

  6. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...interaction between IgE antibodies and eosinophils, where the eosinophils release cytotoxins via degranulation...

  7. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...generate covalent bonds with positively-charged amino acids. Unfortunately, the location of biotin incorporation...enzyme biotin ligase, which binds to a small (< 20 amino acid) AviTag sequence in a protein and adds biotin...

  9. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry...

  10. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...studies showed that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series... cultured mammalian cells by osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 ...

  13. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...only requires a small epitope tag (FLAG is eight amino acids, HA is nine), multiple repeats of the tag ...editing and gene activation, is composed of only 529 amino acids, less than half the size of either Cas9 or...

  14. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...best known: it’s derived from the small (19 kDa) luminous shrimp (Oplophorus gracilirostris) luciferase ...Luciola cruciata) Natural 562 64 Luciferin ATP No Luminous shrimp(Oplophorus gracilirostris) Natural 455 ...

  15. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...a small peptide that represents a short, unique amino acid sequence found in the protein, or, are injected...a single epitope, so it’s less likely that this amino acid sequence will be shared across other proteins...

  16. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...gRNAs used the RNA nuclease Csy4 from Pseudomonas aeruginosa to cleave 4 gRNAs separated by 28 base Csy4 recognition...processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part of their CRISPR...

  17. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F (2013) ...

  18. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...under control of the arabinose-inducible promoter. Cells are first induced with arabinose to begin expressing...

  19. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ... antibodies, such as inclusion of non-canonical amino acids or peptide tags. 5) Strong protein-protein...Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for Conjugation of Effector Molecules...
Showing: 61 - 80 of 262 results