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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 163 results for: Kars

Showing: 61 - 80 of 163 results

  1. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG...Mycoplasma Our 293T cell line was obtained directly from Takara, and is routinely tested for mycoplasma contamination...

  2. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...However, approximately 40% of human (and most eukaryotic) proteins localize to chemically distinct subcellular...fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway."Biochemical and biophysical...

  3. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...interactions. What is FRET? In their JCB 2003 paper, Sekar and Periasamy defined FRET as "a distance-dependent...May; 34(5):341-50. doi: 10.1002/bies.201100098. Sekar RB, Periasamy A. Fluorescence resonance energy transfer...

  4. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic organism, is a commonly used model organism in...double every 2 hours. Yeast are also the first eukaryotic genome to be entirely sequenced and is very amenable...

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...https://doi.org/10.5607/en20006 Rolland, A.-S., Kareva, T., Yarygina, O., Kholodilov, N., & Burke, R. ...

  6. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana... M., Gotthard, G., Tünnermann, J., Tänzer, T., Karpenko, J., Mertes, N., Xue, L., Uhrig, U., Reinstein...

  7. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...Laboratory. Vancouver, BC Canada 49.2827°N 123.1207°W – Karissa Milbury I attended the march in Vancouver, BC,...science in the public sphere as often as they can. Karissa Milbury is currently a PhD candidate at the BC ...

  8. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. It can be very time consuming...Medicine (CMM), Department of Medicine, Solna, Karolinska Institutet, in Sweden. He has a particular interest...

  9. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...for PCR and surveyor. Q15: I used DNA polymerase Takara Ex Taq™ DNA Polymerase for my genomic PCR, but ...successfully used Herc II or Kapa Hifi in PCR. Maybe this Takara enzyme is not very robust in this case for EMX1...

  11. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...pAAV-Ef1a-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth New viral tool AiP13987: pAAV-AiE0452h...

  14. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...avenue of modifying and manipulating endogenous eukaryotic RNA.” Endless possibilities with PUF proteins...

  15. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...introduction of nucleic acids into bacteria or eukaryotic cells is a common technique in molecular biology...

  17. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids...

  18. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ... your imaging time to get enough signal. This workaround risks cellular phototoxicity and eliminates the...
Showing: 61 - 80 of 163 results