We narrowed to 109 results for: LYC;

1. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

   Type

   Blog Post

   Published

   Dec. 13, 2018, 1:14 p.m.

   ...viral particle’s affinity for heparan sulfate proteoglycans on the surface of the packaging cells (Vandenberghe...

2. Antibodies 101: Chimeric Antibodies

   Type

   Blog Post

   Published

   April 25, 2023, 1:15 p.m.

   ...blog Antibodies 101: Validation Antibodies 101: Polyclonal Antibodies Antibodies 101: Selecting the Right...

3. Addgene Begins Distribution of Recombinant Antibodies

   Type

   Blog Post

   Published

   March 28, 2022, 4:30 p.m.

   ...hybridomas’ genomic drift or limited material from polyclonal animal preparations (Bradbury et al., 2015). ...

4. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

   Type

   Blog Post

   Published

   Sept. 14, 2021, 1:15 p.m.

   ...backbone. Test multiple colonies before saving a glycerol stock to ensure the colony you selected contains...

5. Hot Plasmids - January 2023

   Type

   Blog Post

   Published

   Jan. 31, 2023, 2:15 p.m.

   ...amplify your signal - giving you the benefit of a polyclonal, with the reproducibility of a (recombinant) ...

6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

   Type

   Blog Post

   Published

   April 7, 2022, 1:15 p.m.

   ...pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol anchor. SGZ contains a PDGFR transmembrane...

7. Reagent Repositories Are Speeding up Science During the Pandemic

   Type

   Blog Post

   Published

   Aug. 4, 2020, 1:15 p.m.

   ...Wang J, Qian Z (2020) Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune ...

8. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   Published

   Aug. 28, 2014, 3:34 p.m.

   ...your digest reactions before loading them. The glycerol in the buffer will make sure your sample settles...

9. Viral Vectors 101: Viral Vector Elements

   Type

   Blog Post

   Published

   July 25, 2023, 1 p.m.

   ...expression of the vesicular somatitis virus G glycoprotein (VSVG). VSVG is a broad tropism envelope protein...

10. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ...individually (~20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing...

11. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

12. Antibodies 101: Isotypes

    Type

    Blog Post

    Published

    Oct. 19, 2021, 1:15 p.m.

    ...lot of ways to classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But...

13. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ...available in bacterial expression plasmids, glycoproteins and other proteins that require post-translational...

14. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...groups from cysteines, or carbohydrates from glycosylation (Dennler, et al., 2015). However, there are ...

15. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...is a little simplified): Find the appropriate glycerol stock within our freezers Pick the sample into...

16. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation. All of these methods ultimately...

17. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...VSV-G and the baboon endogenous retrovirus Rless glycoprotein (BaEVRless) had high rates of transduction for...

18. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ... the plasmid that should be used to maintain a glycerol stock. Choose from: ampicillin, apramycin, blasticidin...

19. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ... with the IPD of an anion channel, such as the glycine receptor, will promote hyperpolarization. PSAM ...

20. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...9 methylation, while the K27 reporter uses the polycomb chromodomain to recognise lysine 27 methylation...