Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 469 results for: NOL

Showing: 61 - 80 of 469 results

  1. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...generating interesting drug-like compounds especially cardenolides which have been used to treat heart disease....disease. Caterpillars that eat milkweed generate cardenolides that tastes yucky (and is poisonous) for bird ...with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke) No methods were available to... to create targeted gene mutations in anoles lizards or in any reptiles at all. But new success is coming... used study the genetic basis of evolution in immunology, neurobiology, and more. Prochlorococcus (Penny...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...antibodies: the power of in vitro display technologies. Nat Biotechnol 29:245–254 . https://doi.org/10.1038...of Milstein and Köhler who developed hybridoma technology. But what about single chain fragment variables...Clinical Application. Clinical and Developmental Immunology 2012:1–15 . https://doi.org/10.1155/2012/980250...(2016) The birth of monoclonal antibodies. Nat Immunol 17:S13–S13 . https://doi.org/10.1038/ni.3608  Monnier...

  3. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ... editing technologies have been shown to be excellent alternative genome editing technologies especially... single-walled carbon nanotube carriers. Nat Nanotechnol 14:447–455 . https://doi.org/10.1038/s41565-019...confers heritable resistance to powdery mildew. Nat Biotechnol 32:947–951 . https://doi.org/10.1038/nbt.2969...Applications of CRISPR–Cas in agriculture and plant biotechnology. Nat Rev Mol Cell Biol 21:661–677 . https://...

  4. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    Published
    May 24, 2016, 2:30 p.m.
    ...Horticulutre Biotechnology, he did his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan ...works as a lecturer in molecular genetics and biotechnology for Horticulture and Forest Crops at the Lilongwe...sciences and agriculture 5:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10...

  5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...Assistant at MGMs Institute of Biosciences and Technology. If you are reading this article then you probably...Mycoplasma Removal Agents (MRA) are derivatives of the quinolone family of antibiotics and are broad-spectrum antibiotic...culture media. Also, drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline...Assistant at the MGMs Institute of Biosciences and Technology in India. Jadhav has a Master's degree in Molecular...of Milan, Italy) and a Bachelor's degree in Biotechnology.       References Degeling MH, Maguire CA, ...

  6. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...once you are up to speed on the basics of the technology. In this blog, we will highlight some common ...applications and kits. Keep in mind that this technology is useful for a variety of purposes beyond what...expression in yeast MoClo is also for eukaryotes! The technology has been used to optimize protein secretion and...Heterologous Proteins in Yeast Mitochondria.” BMC Biotechnology, vol. 17, no. 1, Springer Science and Business... Enables Rapid Assessment of Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023...

  7. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...are swarming to see what can be done with this technology to help patients. Dr. Stoffels and others in ...months compared to a year or greater with older technologies. Addgene is proud to be helping get CRISPR reagents..., the panelists agreed that, for example, the technology is already available today to cure sickle cell...really sophisticated”.     New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History...

  8. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...It’s clear that CRISPR-Cas9 technology has really changed the game for anyone looking to quickly and ...question. Additionally, a concurrent Nature Biotechnology article describes the development of a mouse...accessible before are now accessible using this technology.” GeCKO library for genomic screens Using lentivirus... Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs...

  9. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...survival tactics of prokaryotes to deliver the technological biological blockbuster known as the CRISPR (...multiplexibility, and ease of use. In order for this technology to survive the diverse demands of the biotech...various innovative biological and translative technologies. Addgene has empowered researchers to harness... idea or application that involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo,...

  10. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...the chance to win some of the latest tools and technologies in life sciences research. The rare disease ...countries. “Over $600,000 worth of cutting-edge technologies were awarded to study 31 rare diseases,” said...ll combine induced pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit...those mutations, they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models carrying...

  11. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...DNA sequences with twin prime editing. Nature Biotechnology, 40(5), 731–740. https://doi.org/10.1038/s41587...deletions using paired prime editing. Nature Biotechnology, 40(2), 218–226. https://doi.org/10.1038/s41587...optimized, paired pegRNAs in plants. Nature Biotechnology, 39(8), 923–927. https://doi.org/10.1038/s41587...pegRNAs improve prime editing efficiency. Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587...cleavage using CRISPR-directed integrases. Nature Biotechnology, 41(4), 500–512. https://doi.org/10.1038/s41587...

  12. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...many opportunities to learn about and develop technologies that will have impacts on many different facets...ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around the poster... addition to the researcher using sequencing technologies to study microbiome interactions with Alzheimer's...genes to many different cell types with minimal immunological effects. AAVs are used in experiments targeting...

  13. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ... Figure 1: An image from AAV Purification by Iodixanol Gradient Ultracentrifugation First, you’ll... on our protocol page for AAV Purification by Iodixanol Gradient Ultracentrifugation. The reader may be...text: "The left panel is a profile photo of an iodixanol gradient after ultracentrifugation. The interfaces...of the 40% layer containing the virus and 60% iodixanol layer, with the bevel pointed up. A syringe at...

  14. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ... the best product-market fit for this set of technologies, effectively communicating the advantages embodied...successful if they are 100X better than current technology and get customers to change behavior. When you...raised and the company may begin to prove the technology. This transition takes around 1 – 2 years. ... The product is the measure of success. Technology is proven in (A) Productize, (B) Roll out to market...

  15. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...channelrhodopsin-based optogenetics minimally-invasive, Frances Arnold’s lab and Viviana Gradinaru’s lab used machine learning...design challenges To overcome these challenges, the Arnold and Gradinaru Labs developed a machine-learning...guarantee their success in vivo. Therefore, the Arnold and Gradinaru Labs expressed ChRgers in mice to..., Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin...

  16. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...innovations” stimulates excellence in science and technology by integrating sex and gender analysis into research... disease being treated, and other hormonal, immunological, and environmental factors. How to include sex...international expert on gender in science and technology and currently directs the EU/US Gendered Innovations...Nations on the topic of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the ...

  17. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    Published
    April 22, 2015, 2:06 p.m.
    ...Voigt: The top products out of biotechnology today are things like butanol - very simple molecules - a few...it, his lab at the Massachusetts Institute of Technology has been “working on new experimental and theoretical... collision that’s taking place between CRISPR technology and synthetic biology, the tools he’s making ...

  18. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...recombination. What is Cre-lox? The Cre-lox system is a technology that can be used to induce site-specific recombination... lethality. To facilitate the use of Cre-lox technology, transgenic mice have been constructed that express...system is another site-directed recombination technology very conceptually similar to Cre-lox, with flippase...analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective alternative to Cre-lox, and...

  19. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org...virulence plasmid are now used routinely in biotechnology to transform plant cells. Degradative plasmids...Nature’s toolbox for new and exciting molecular technologies!  This post was originally written by Jessica...

  20. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells remain difficult...using peptide-assisted genome editing. Nature Biotechnology, 10.1038/s41587-023-01756-1. https://doi.org...monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org/10.1038/s41587-023-02018...throughout the non-human primate brain. Nature Nanotechnology, 18, 1241–1251 (2023). https://doi.org/10.1038...
Showing: 61 - 80 of 469 results