We narrowed to 228 results for: Ott;
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TypeBlog PostPublishedOct. 5, 2023, 1:15 p.m....controls, which we will briefly touch on. Western blotting When comparing samples in different lanes in a... P. H., et al. “Goals and Practicalities of Immunoblotting and Immunohistochemistry: A Guide for Submission...
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27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m....recently deposited with Addgene by Dr. Mark Prescott. Dr. Prescott and Dr. Rod Devenish (both at Monash University...use. The plasmids were validated by both Western blotting and immunohistochemistry, and are summarized in... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m....piggyBac was discovered in the cabbage looper moth (Potter et al., 1976). Its target site is TTAA and unlike...genome. References and Resources References Feschotte C, Pritham EJ (2007) DNA Transposons and the Evolution... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....commonly used for every cell culture. Reusing the same bottle of serum again and again for each subculture can...Water baths, liquid nitrogen containers, reagent bottles and other sources of infection must be cleaned ... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....H., Horvath, P., Moineau, S., Mojica, F. J. M., Scott, D., Shah, S. A., Siksnys, V., Terns, M. P., Venclovas...Makarova, K. S., Koonin, E. V., Cheng, D. R., & Scott, D. A. (2018). Functionally diverse type V CRISPR-Cas... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....about how protein networks work in cells. A major bottleneck, however, to these IP-based assays is to find... 32: 601-618. PubMed PMID: 9928493. 4. Liang X, Potter J, Kumar S, Ravinder N, Chesnut JD. 2017. Enhanced... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m.... and Continue to Next Step" (blue button at the bottom of Figure 6) or you can click the "Save" button...Be sure to press the white "Save" button at the bottom of the page before moving onto a different page... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....protein-based buffers, so here’s a quick tip: Keep a bottle of chemical buffer around, which works well and...Stacked Film Exposure Improve Sensitivity in Western Blotting. Journal of Pharmacological and Toxicological ... -
Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog PostPublishedDec. 11, 2017, 2:02 p.m....disease and open science: Interview with Darrell Kotton Find additional plant biology blog posts Resources... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog PostPublishedFeb. 6, 2025, 2:15 p.m....protein for pseudotyping NanoMEDIC particles Akitsu Hotta pHCMV-RabiesG Contains Rabies virus G protein... -
New Videos: Addgene Lab Tips
TypeBlog PostPublishedFeb. 15, 2018, 2:41 p.m.... plasmids since 2004. In that time, our lab has gotten a lot experience in processes including automated... -
Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)
TypeBlog PostPublishedAug. 17, 2021, 1:15 p.m....occasion. Disaster in the lab you haven’t quite gotten over? We have you covered. Believe us.... -
Visualizing Your Plasmid Request Data
TypeBlog PostPublishedApril 11, 2023, 4 p.m....explore how I could visualize the aggregated data. Plotting the data in interactive figures is a great step... -
Editor's Choice, October 2016
TypeBlog PostPublishedNov. 4, 2016, 2:30 p.m....Outreach Scientists to Colorado, Berlin, Atlanta, Ottawa, Norwich, and Hannover, we raised money for the... -
Addgene’s Blog Gets a New Look!
TypeBlog PostPublishedJan. 25, 2021, 5:54 p.m....will have the author information at the top and bottom of their article. We hope you’ll join the conversation... -
MassBioEd Career Exploration Day at Addgene
TypeBlog PostPublishedNov. 13, 2015, 3:30 p.m....our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how much work is... -
Addgene's Website Update
TypeBlog PostPublishedApril 8, 2024, 2:15 p.m....what you think, through the yellow popup at the bottom of the page. Happy surfing! This post was written... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog PostPublishedNov. 10, 2015, 3:30 p.m....methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein... -
Keeping Up With CRISPR/Cas9
TypeBlog PostPublishedMarch 11, 2014, 5:55 p.m....just how big and mainstream the news on CRISPR has gotten, consider these headlines in The New York Times... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m...., in collaboration with “cloning guru” Kathleen Ottina and David Sabatini at the Whitehead Institute and...