We narrowed to 84 results for: PEP

1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions. ...

2. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   .../35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic...

3. Open Resources and Plasmid Tools For Studying C. elegans

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   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the split ...

4. 3D Printing Meets CRISPR Cas9

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   Blog Post

   Published

   Oct. 17, 2017, 12:55 p.m.

   ...the surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said...

5. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...Baltimore. She is particularly interested in signaling peptide maturation in neural and endocrine cells. She can...

6. Which Fluorescent Protein Should I Use?

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   Blog Post

   Published

   May 20, 2014, 2:06 p.m.

   ...molecular and cellular processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov et. al. (2010) “Fluorescent...

7. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...promise that we could instead have things like peptide or RNA aptamers that act as genetically encoded...

8. Antibodies 101: The Four ELISAs and When to Use Them

   Type

   Blog Post

   Published

   July 25, 2024, 1:15 p.m.

   ...sample of wild type cells, purified protein, or a peptide. The positive control will confirm that the procedure...

9. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...their broad range of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells (even...

10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

11. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...library of AAV9 mutants that had a small randomized peptide loop inserted in the AAV9 capsid. This mutant library...

12. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...residue of a protein or knocking out a very small peptide. In these cases, you have little flexibility as...

13. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...PubMed PMID: 2725683. 2. Miller, C.J., Kassem H.S., Pepper S.D., Hey Y., Ward T.H., Margison G.P. “Mycoplasma...

14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type

    Blog Post

    Published

    Oct. 27, 2015, 3 p.m.

    ...PMC2784028. 3. Munro, Sean, and H. R. Pelham. "Use of peptide tagging to detect proteins expressed from cloned...

15. Hot Plasmids: Summer 2024

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    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ... A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single ...

16. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ...or (ii) designed to form an inert, unstructured peptide and therefore consisted of glycines, serines and...

17. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    Published

    Oct. 11, 2016, 2:30 p.m.

    ...nanostructures (Zhang et al., 2019) or cell-penetrating peptides (Santana et al., 2020) and plants viruses (barley...

18. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ...PV), somatostatin (SST), vasoactive intestinal peptide (VIP), or lysosomal-associated membrane protein...

19. A Control for All Seasons

    Type

    Blog Post

    Published

    Oct. 5, 2023, 1:15 p.m.

    ...with its antigen or a small epitope-containing peptide to block the antigen-binding site. The sample is...

20. Antibodies 101: Validation

    Type

    Blog Post

    Published

    March 24, 2022, 1:15 p.m.

    ...specific, then you expect that the bulk of the peptides identified to be from the target protein. This...