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... of a two-part interview with Vini Mani and Amy Gilson from Science in the News at Harvard University....your science, we sat down with Vini Mani and Amy Gilson from Science in the News, a graduate student organization...Podcast Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani is a graduate student at Harvard University...News, and member of the SITN podcast team.     Amy Gilson recently received her PhD from Harvard University...SITN podcast team. You can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer...McFadden Learn about SITN's work at DayCon 2015 5 Reasons to Use Reddit for Science Communication ...

...changes in specificity than AAV-PHP.eB. For this reason, the Gradinaru lab anticipates that high-dose AAV-PHP.AX...happy transducing! This post was written by Rachel Leeson, with significant contributions from Cynthia Arokiaraj...Altermatt, M., Dobreva, T., Chen, S., Wang, A., Thomson, M., & Gradinaru, V. (2021). Deep Parallel Characterization...s41565-023-01419-x Deverman, B. E., Pravdo, P. L., Simpson, B. P., Kumar, S. R., Chan, K. Y., Banerjee, A....., Wang, Q., Katz, N., Buza, E. L., Bell, P., & Wilson, J. M. (2018). The Neurotropic Properties of AAV-PHP.B.... J., Bell, P., Raymond, A., Stanford, W. L., & Wilson, J. M. (2019). The GPI-Linked Protein LY6A Drives...journal.pbio.3002112 Jang, M. J., Coughlin, G. M., Jackson, C. R., Chen, X., Chuapoco, M. R., Vendemiatti,...

...them for use in your lab. Structural Comparison Functional Comparison Advantages of Nanobodies How to Use...for a comparison of nanobody, HCab, and traditional IgG antibody structures. Functional comparison Strong...Nanobody Toolbox Find Nanobody Plasmids Structural comparison As the name suggests, nanobodies are smaller ...Large: Advantages of Antibodies One of the main reasons antibodies are great indirect immunodetection tools... antibody incubation. See figure 4A below for comparison of this one-step staining with nanobodies vs ...

... is adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify...DNA gel extraction method described in Kim and Morrison is nearly identical to that of commercially available...Purify DNA using glass filters. Image: Kim and Morrison, 2009. This method provides DNA yield and quality... kits for different purposes, with the Kim and Morrison method, you can just adjust the number of glass... protocol video library References Kim, Y., & Morrison, S.L. (2009). A Rapid and Economic In-House DNA...://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason, M.G., Wang, Y., Wee, E.J., Turni, C., Blackall,...

...out their own novel experiments in semester two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates...capabilities and laboratory and critical thinking skills (Knutson et al., 2010a,b). Classroom-based research projects...before a CURE trainee goes on to win a Nobel!   Jason Pellettieri is Professor and Chair of the Biology...Einstein Discovers America. New York./  Callahan, K., Peterson, C., et al. 2022. External collaboration results...planarians. Developmental Biology. 457:119-127. Knutson, K., Smith, J., Wallert, M., Provost, J. 2010a....and Molecular Biology Education. 38(5):317-323. Knutson, K., Smith, J., Nichols, P., Wallert, M., Provost...Education. 38(5):324-329. Kowalski, J., Hoops, G., Johnson, J. 2016. Implementation of a collaborative series...

...troubleshooting as advised by the technical support person. This delayed the progress of the project. After...familiarize yourself with technical support. Even having personal experience with technical support through the ...general positive attitude, and an open-minded personality. These traits will help you deal with any incoming...and there are also differences in customers’ personalities and their expectations. If you are willing to...is essential for success. For possibly this same reason, it can be one of the more constant and safe corners...simple way even if you don’t exactly know why. Personal and professional development in technical support...support gives you the opportunity to develop interpersonal and communication skills. Problem solving and...

...therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile genetic element that transposes...heterogeneous iPSC cell lines, which can complicate comparisons made between lines. Incomplete silencing of transgenes...heterogeneity between clones. This can complicate comparisons made between samples, i.e. transgene expression... into cells as a donor plasmid containing the transposon, along with a helper plasmid that expresses the... is that genomic alterations can occur at the transposon insertion site, so sequence-verification is required... pluripotent mouse stem cells by the piggyBac transposon." Nature Methods 6, no. 5 (2009): 363-69. PubMed...Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak M. Gupta, Mei Huang, Zongjin ...

...multiplex (Thellwell, 2000). See Table 1 for a comparison of features of several commonly used chemistries...sixfold variation in expression levels reported (Biassoni, 2014), so you’ll want to ensure that your reference...genes - and it is well worth your time to do one (Biassoni, 2014). At the bench As a highly sensitive technique... See Table 2, (adapted from Wong, 2018) for a comparison of different analysis methods.   Method ...defined log-linear phase   Table 2: A comparison of different qPCR analysis methods. Table adapted... master. References and Resources  References Biassoni R; Raso A. (eds.), Quantitative Real-Time PCR:...doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes V; Garson JA; Hellemans J; Huggett J; Kubista M; Mueller ...

... of a two-part interview with Vini Mani and Amy Gilson from Science in the News (SITN) at Harvard University...half of our conversation with Vini Mani and Amy Gilson from SITN, we discuss some of the many things you.... Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani is a graduate student at Harvard University...You can follow Vini on twitter @vinidhra.     Amy Gilson recently received her PhD from Harvard University...SITN podcast team. You can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer...Science Careers 10 Steps to a Perfect Science Talk 5 Reasons to Use Reddit for Science Communication ...

...arise as a result of existing professional (and personal) relationships. However, most job seekers will...pile, it may never even be reviewed regardless of personal connections to the hiring group.  Read here for... Concern” as you will not likely know the exact person monitoring submissions (usually a Human Resources...mutual connection. If the organization is small, any person at the company might be able to help you. If the...LinkedIn is one great way to find an appropriate liaison, but you can use alumni databases, friends, and...make a difference. Having your resume forwarded personally gives you a much better chance at an interview...

...competence procedures Threats to personal standing – including undermining personal integrity, destructive innuendo... analytic scientists seem to toss data-driven reasoning out the window when making decisions. We think...think scientists make choices based on logic and reason, but often our decisions are based on emotions and... these labs in the first place. This is another reason to do your research and choose wisely. From the...members fight it out PI intervenes if there are interpersonal conflicts PI has no idea who is dating whom ...choose a grad school or postdoc lab. Having a comparison can be very helpful in uncovering strengths and...

...University of Alberta studying with the Szymanski lab. Reasons to study how a phage recognizes its host Bacteriophages...molecules in molecular biology labs (for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages...following is a description of a technique published by Simpson, Sacher, and Szymanski in January 2016 in Viruses... for physical shearing exist as well, such as sonication and needle shearing. Essentially, any method ...Callum J., Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based...27536293. PubMed Central PMCID: PMC4971087. 3. Simpson, David J., Jessica C. Sacher, and Christine M. ...Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David J., Jessica C. Sacher, and Christine M. ...

... James M. Wilson New serotype pENN.AAV.hSyn.Cre.WPRE.hGH AAV2 Recombinase James M. Wilson New serotype...James M. Wilson New serotype pENN.AAV.CamKII 0.4.Cre.SV40 AAVrg Recombinase James M. Wilson New serotype...FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New viral prep pAAV-CaMKIIa-hM3D(Gq)-mCherry...resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the Addgene...

...PMC4246457. 4. Davidson CJ., et al. "Improving the limit of detection for Sanger sequencing: a comparison of methodologies...although this has been improved in some studies) (Davidson et al. 2012, Tsiatis et al. 2010). As the field... approaches can provide the researcher with a reasonable level of certainly that the effects they see ... PhD candidate in the laboratory of Prof Steve Jackson at the Gurdon Institute at the University of Cambridge...Vouillot, Léna, Aurore Thélie, and Nicolas Pollet. "Comparison of T7E1 and surveyor mismatch cleavage assays...PubMed Central. 5. Tsiatis, Athanasios C., et al. "Comparison of Sanger sequencing, pyrosequencing, and melting...

...such a resource when updates depend on a single person. With thousands of scientists routinely using and...Protein Property Visualization allows for visual comparison of FPs and how they relate to one another. .  ...you or your lab use frequently) for the sake of comparison or ease of retrieval.  These collection pages...of your choosing!), and they can be downloaded in JSON or CSV format to quickly retrieve all of the information...store and represent within-study photobleaching comparisons.  We very much appreciate feedback, so try out...plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein Collection ...

...be a graduate student, postdoc, professor, etc. Reasons to pursue a visiting role The main motivation to...can’t obtain at your current institute. A common reason is hands-on training in a technique that only a...hard to find, or inaccessible to you for another reason. Visiting would allow you to train and acquire ...data you need on the new instrumentation. Bonus reasons Visiting gives you access to possible new collaborations... willing to host you! If there is someone you personally know or your lab collaborates with that can fulfill...work together to ensure your expectations are reasonable, your reagents are onsite before you need them...

...Non-science favorites  The Mistborn series by Brandon Sanderson "The Mistborn series is the first 3 books of a...funny, bittersweet, and shocking at times."     - Jason Snair, Product Designer Sarum: The Novel of England...skeptical reader of historical fiction (I can personally attest to this!). Following history from the ...participated in a book club before, but I had a few reasons for launching this one. On a purely selfish level...contentious, discussions. I presented the first book, Nicholson Baker’s The Anthologist. The book ended up literally...Prejudice, and some more obscure treasures, like Tove Jansson’s The True Deceiver. There have been books that ...

...If cloning methods had personalities, SLIC (sequence- and ligation-independent cloning) would be a true...also be beneficial. SLIC is most often compared to Gibson assembly, a cloning method also based on homologous... recombination. The higher temperature at which Gibson assembly takes place may limit formation of secondary... of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase is much less...less expensive than the enzymes required for Gibson assembly. SLIC is a standardized method for multi-fragment... Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning...

... Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB (2017) A stable but reversible integrated...assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA in 2017 to enrich and identify...Wen et al., 2018). SRIRACCHA uses the Piggybac transposon to stably integrate a reporter gene that contains...your cells.  A plasmid that contains Cas9. The Madison lab also created an inducible SRIRACCHA system ... experiment that targeted the gene NFE2L2, the Madison lab found that H2B-GFP expressing cells exhibited...

... think more favorably of scientists they know personally Scientists in your field will know about grants...Job hunting outside of academia is all driven by personal connections What can you do to get the most from...whose company they enjoy on a professional and personal level.  Here are some tips to get out of the rut...free places to order cards. There are a few good reasons to have a card but two of the best are: if you ...with a more general intro until you know if the person you are talking to is really in your direct area...attend. Meet some people online to follow up with in person. Email lab members that have moved on to see if...