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Showing: 61 - 80 of 245 results

  1. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...

  2. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...Hideyuki Okano presented ground-breaking work on spinal cord injuries; Jessica Young shared research on..., a half-brother who is a quadriplegic due to a spinal cord injury, and numerous family friends who had...

  3. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe, 2022) and substantia...that are physically blocked, such as those in the spinal cord. Now that you understand this new tool, go...

  7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ... In frogs, ectopic expression of ion channels, despite having an otherwise normal genomic background, ...Subsequent cutting results in a two-headed worm, despite the fact that it’s genetically wild type  (Oveido...

  8. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...for your next experiments, they will also likely inspire further-improved versions. Thanks to Christophe...Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids...

  9. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-... can be used for extra security. Finally, when spinning down lentivirus, you’ll need to use centrifuge...

  10. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...postdoctoral issues first at Brigham and Women’s Hospital/Harvard Medical School and then Boston University...communication and learning to work with people despite the distance improved my verbal and written communication...

  11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...the picture had a broken controller and would not spin. It was going to cost far more than it was worth...microcontroller and a motor controller and made it spin again!  We’ve also learned to be resourceful and...

  12. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...doing this with very little money. So when did inspiration strike us so hard that we upended our lives and...in fact, our color changing flower project was inspired by a Facebook comment.  In our day job, we worked...

  13. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...) using a high-speed ultracentrifuge. During the spin, the virions will form a band due to their high ... https://doi.org/10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. ...

  14. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...die from cancer-related complications each year. Despite these grim statistics, new strategies for attacking...cancers, especially those of the pancreas and colon. Despite the prevalence of Ras mutations in a variety of...

  17. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...now available. In this post, we’ll review the inspiration behind ExM, how it works, and some of the limitations...improve label density (Mikhaylova, et al. 2015).  Despite these limitations, ExM is a valuable tool, especially...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...follow in the footsteps of Luxturna™. However, despite promising results in preclinical studies some potential...diseases such as hemophilia, muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and...

  19. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons have been...can also be combined in single-plasmid INTEGRATE (pSPIN), which offer simple transformations for smaller...

  20. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...beta-lactam resistant population to the antibiotic, despite this resistance gene being encoded by a high-copy... system to target virulent S. aureus, a common hospital-borne infection. Two rounds of phagemid treatment...
Showing: 61 - 80 of 245 results