We narrowed to 868 results for: TOR

...two classes of base editors were cytosine base editors (CBEs) and adenine base editors (ABEs). CBEs mediate... first cytosine base editors from Alexis Komor and the first adenine base editors from Nicole Gaudelli...cytidine deaminase portion of the base editor to create SpCas9 base editors with editing windows as small as...Fourth-generation cytosine base editors The fourth-generation base editors, BE4, reduce the undesired C→... base editors The beginnings Nicole Gaudelli from David Liu’s lab created an adenine base editor that ...obtained four adenine base editors (ABEs). ABE7.10 is the most active editor, displaying an average editing... adenine base editor with improved nuclear localization and expression. This base editor was named ABEmax...

... maintain high standards for both our depositors and requestors.  Requesting Plasmids available for Packaged...through a distributor?  Yes! Packaged on Request service will be available through our distributor partners...excited to announce that we are expanding our viral vector service to include a Packaged on Request option...AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes....requesting and ordering a Packaged on Request viral vector prep.    Excited? So are we! This expansion...ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be available...available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request service...

...and the pRepCap plasmid containing replication factors and serotype-specific capsid genes.  In this helper-free...you’ll need to determine the titer of your viral vector solution. A variety of titering methods have been...preparation.  rAAVs  Now your AAV virions are ready to store or use. They are quite stable and will withstand...about common AAV applications by reading our Viral Vectors 101 series!  This post was originally written by... Unpurified Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular Biology. https://doi.org/...the Stability and Inactivation of AAV Serotype 1 Vectors. Human Gene Therapy Methods, 28(1), Article 1. ...(2021). Inactivation of Adeno-Associated Viral Vectors by Oxidant-Based Disinfectants. Human Gene Therapy...

...edition of our Viral Vectors 101 eBook!  This practical guide to using viral vectors in the lab is a helpful...introduction to viral vectors and their elements; resources on different types of vectors, capsids, and tools...information on everything from producing your own vectors in the lab to optimizing AAVs in your experiment...edition Resources on addgene.org  Addgene's Viral Vectors Guide Addgene's Chemogenetics Guide Addgene's Optogenetics...

...Therapeutics, co-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith Joung, PhD...to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role of the MECP2...exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements, and ...& Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have suggested ...neurological deficits observed in Rett Syndrome. Restoring the MECP2 gene by excision of the Stop cassette...precise amount of protein to each brain cell to restore physiological levels. Much research is now focused...researchers developed adeno-associated viral (AAV) vectors for the targeted delivery of a functional MECP2...

...plasmid tools! Thank you depositors! In fact, this year, Addgene depositors quickly stepped up to make... as an example). Depositor Week will be fun for everyone, whether you are a depositor or not. We will ...Tuesday: Depositor features (plasmid deposits and blog posts showcasing the work of depositors) Wednesday...for some Addgene depositor fun facts for a quick way to grasp the big impacts depositors have on science...your calendars for November 16-20, 2020 for #DepositorWeek on Twitter!  That’s today through Friday. There...scientists who make a big impact in open science. Depositors consistently contribute to our collection so ...COVID-19 related plasmids currently in Addgene’s repository which have been requested about 1,500 times from...

...deposited into repositories, and financial support to maintain repositories’ capacity for storing, validating...through a repository, they remain accessible during these times. Centralized reagent repositories, also known...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the rapid... the scientific community can support repositories. Repositories are essential for the advancement of ...original sources. Figure 1: Biological repositories that distribute mouse strains, plasmids, cell...Culture Collection (ATCC) is a private, nonprofit repository for a variety of materials for life science research... sharing and distribution of cell lines from repositories on opposite sides of the world shows that science...

...ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV and lentiviral...Serotype Testing AAV inventory for the entire collection! Plasmid Serotype Name Depositor 27056 AAVrg pAAV-Ef1a-DIO...pAAV-EF1a-Flp-DOG-NW Connie Cepko Viral vectors coming soon! These vectors listed below should be released at... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...mDlx enhancer element. Plasmid Serotype Name Depositor 75470 AAV1 pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry...promotes neuronal firing. Plasmid Serotype Name Depositor 44361 AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan...also available in AAV1. Plasmid Serotype Name Depositor 69570 AAV1 pAAV-EF1a-N-CretrcintG Connie Cepko...

...block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial constitutive...research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively high transduction... many more useful viral vectors available at Addgene: Follow our viral vector blog posts for updates on...tips for using retroviral vectors in your experiments. Gamma-retroviral vectors Popular gamma-Retroviral...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...

... download the PEI calculator for AAV packaging Use the PEI transfection calculator for AAV packaging ...corresponding column in the calculator spreadsheet: We hope that this calculator will make it easier to plan...for AAV packaging, considering using this PEI Calculator. AAV packaging typically requires transfecting...right, you need to do a few calculations. This calculator can do the math for you and it’s simple to make...whole experiment And here’s the equation the calculator uses to give you the μl needed of each plasmid...experiments. Let us know what you think about the calculator in the comments section below. References 1....Production of Recombinant Adeno‐Associated Viral Vectors for In Vitro and In Vivo Use." Current Protocols...

...science stories. Here are some of our favorite spooky science stories. Six spooky science stories Zombie...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways ...Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green...forcing it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then... "Back from the dead; the curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." ...

...ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have...Testing AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give...Calcium Sensors). We are expanding the inventory of these vectors to more serotypes. We currently package...GCaMP7s. See all our biosensors inventory, and check out our latest vectors below: pGP-AAV-syn-jGCaMP7f-...addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV inventory here. pAAV.hSynap.iGABASnFR...support by growing our inventory of recombinases and recombinase-dependent vectors. We recently released...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...

...guarantee certain rights and protections for depositor and requestor alike. That’s why Addgene works closely...closely with TTOs to help protect our depositors’ intellectual property, and to help requesting institutions...California Institute of Technology Cold Spring Harbor Laboratory  Dana-Farber Cancer Institute  Fred Hutchinson...

...ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have...pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors New optogenetics inhibitors Plasmid Serotype Name 99039 ...AAV1 pAAV-ihSyn1-DIO-tTA Viral vectors coming soon! These vectors listed below should be released at... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...few months. You can also browse our entire AAV inventory. Our new AAVs include: Control AAVs for serotype...always excited to add new control AAVs to our inventory since we know that scientists need these tools...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs Plasmid...

...ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have... used in conjunction with optogenetic activators and inhibitors that respond to blue light. We recently...GABAergic interneurons. These vectors can also be used as the control vectors for experiments encoding a ...pAAV-hSyn-mCherry at Addgene. Viral vectors coming soon! These vectors listed below should be released at... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...far in 2019. You can also browse our entire AAV inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE... Addgene. GABA sensor AAV GABA is the primary inhibitory neurotransmitter in vertebrates, but methods ...

...blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Pseudotyping Viral Vectors 101: Systemic...and are required for replication, packaging, and vector persistence. They work in conjunction with the ...retained with the gene in a recombinant AAV (rAAV) vector (Figure 1). Everything else outside the ITRs gets...gets left behind.    Figure 1: Recombinant AAV vector with ITRs that flank the gene cassette, which contains... bp version is used in most plasmids. In an AAV vector plasmid, they flank the gene of interest. A single...of the most important elements of AAV and rAAV vectors, modifications can affect gene transfer efficiency...an ITR with a sequence containing transcription factor binding sites can increase transgene expression...

...efficiency can vary with titer, cell type, and other factors, so make sure to put that aliquot to full use! ...

...serotypes utilize a number of different receptors, co-receptors, and other mechanisms to gain entry into...other factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors 101 series...on the Addgene blog Viral Vectors 101: An Introduction to AAV Viral Vectors 101: The AAV Retrograde Serotype...Using AAV vectors in complex biological systems can be tricky at best, and downright infuriating at worst...know what expression pattern you need, and the factors in your system that can affect expression, before...before starting the selection process. Factors to think about are (1) your experimental conditions and measurements...testing, and optimizing three key variables of an AAV vector: serotype, promoter, and titer.     Figure...

...-dependent vectors, this can cause challenges for the experimental design as these vectors can exhibit...your system. Chemogenetic vectors  Expression of retrograde serotype vectors is present at both the injection...expression, and has retrograde functionality to monitor and map neurons (Tervo et al., 2016). With AAVrg...neuronal tracing reagents, AAV particles require receptor-mediated entry into the cell. This means that ...serotype to conduct experiments with chemogenetic vectors, this property must be taken into account since...projection neurons retrogradely transduced by the vector. One method for minimizing the impact of this phenomenon...phenomenon is to use a retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce the projection pathway...

...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ... Viruses are essentially DNA (or RNA) delivery vectors, so what makes them better than the alternatives...measures in a separate, upcoming post. If a viral vector seems like the right approach for your experiment...experiment?           Click Here to Find Viral Vector Resources at Addgene! References and Resources...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed... J, et al. “Altering the tropism of lentiviral vectors through pseudotyping.” Curr. Gene. Ther. 5 (2005...