We narrowed to 746 results for: TRI

...’s catalog — from the recently developed to the tried-and-true classics. Check out our Biosensors Collection...biosensors new to Addgene this year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s...TRansmembrane region-to-IntrAcellular loop linkers” (MTRIA for short), where a fluorescent protein is inserted... ligand (Ino et al. 2022). The oxytocin sensor MTRIAOT enabled real-time observations of oxytocin dynamics...earlier tools. They also produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters...ligands - almost A to Z! - from ATP to urotensin. The MTRIA sensors will be useful for investigating neuronal...sensors for these and other targets. Figure 1: MTRIA biosensors are generated by inserting a circularly-permuted...

...Shortly after though, three groups independently tried reproducing these results and determined that even... expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR,...

...heard stories and read papers wherein a researcher tries 5 separate experiments at once to get confirmatory...procedures like pouring a plate or running a gel have intricacies that aren’t always mentioned in static written...

...filled depends on cellular factors. Multiple labs tried to identify beneficial factors to include in even...bases. Thus, base editors have an activity window restricted to the stretch of ssDNA created by Cas9 binding...

...There is something akin to a frenzy as the crowd tries to weigh and determine the value of every sweet ...This post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software...falciparum (the parasite that causes malaria). I attribute their success to technical expertise, thoughtful...Cas-OFFinder provides a bare-bones data dump of ALL strings in a genome using any IUPAC encoded pattern at ...biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick D., et al. "DNA targeting specificity of RNA-guided...

... antibody manufacturer for wash recommendations. Tris-buffered saline (TBS) or phosphate-buffered saline...abundant protein if you’re stripping, since you’ll lose protein with every strip cycle. Troubleshooting the...Increase volume of wash buffer Incomplete stripping (If stripping/reprobing) Check for antibody signaling...signaling after each round of stripping* Use a harsher stripping buffer Too little signal Large proteins...Western blots can be tricky to get right. If you’ve thoughtfully done the technical design of your blot...need such precise measurements, you can use a colorimetric ladder, visible to the naked eye; a fluorescent... choose from.  Transfer Transfers are a little trickier than gels and may require multiple runs to optimize...

...e0124958. LOC8-ing mitochondrial fusion with a new tri-chromatic assay Georg Ramm’s lab at Monash University...cumbersome. To overcome many of the limitations intrinsic to DNA-guided systems such as TAL effectors or...modified to use different antibiotic resistances, restriction enzymes and plasmid backbones. pUCXKT contains...gene-specific forward primer containing the desired restriction site (the MCS has several options) and a pUCX-specific...resistance cassette, a ribosome binding site, SacI restriction site and a linker region. The PCR product is ...BMC Molecular Biology. 2013 August 20th; 14(1). TRICK: A method for visualizing the first round of translation...the protein product. The system, cleverly termed TRICK, for Translation RNA Imaging by Coat protein Knock-off...

...antibody. TRIM21, an E3 ubiquitin ligase, is the basis of TRIM-away. In normal physiology, TRIM21 recruits...endogenous TRIM21 is sufficient for TRIM-Away, and you only need to deliver the target antibody. TRIM-Away ...Plasmids Podcast! TRIM-Away: Targeted endogenous protein degradation Article contributed by Mary Gearing... proteasome function. TRIM-away compared favorably to drug inhibition, with TRIM-away targeting of mTOR.... To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding that...changes in expression of TRIM21-associated genes, indicating that TRIM21 overexpression does not aberrantly ... methods for TRIM-Away delivery. For small cell populations, co-microinjection of TRIM21 protein and the...

...available at Addgene!"{% endraw %}{% end_module_attribute %}{% module_attribute "child_css" is_json="true" %}...%}{% raw %}{}{% endraw %}{% end_module_attribute %}{% module_attribute "css" is_json="true" %}{% raw %...padding-right":"200px"}{% endraw %}{% end_module_attribute %}{% module_attribute "definition_id" is_json="true...%}{% raw %}null{% endraw %}{% end_module_attribute %}{% module_attribute "field_types" is_json="true" ...style":"group"}{% endraw %}{% end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw... raw %}null{% endraw %}{% end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}...user_generated_content":false}{% endraw %}{% end_module_attribute %}{% module_attribute "module_id" is_json="true" %}...

...plasmid collection"{% endraw %}{% end_module_attribute %}{% module_attribute "child_css" is_json="true" %}...%}{% raw %}{}{% endraw %}{% end_module_attribute %}{% module_attribute "css" is_json="true" %}{% raw %...padding-right":"180px"}{% endraw %}{% end_module_attribute %}{% module_attribute "definition_id" is_json="true...%}{% raw %}null{% endraw %}{% end_module_attribute %}{% module_attribute "field_types" is_json="true" ...style":"group"}{% endraw %}{% end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw... raw %}null{% endraw %}{% end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}...user_generated_content":false}{% endraw %}{% end_module_attribute %}{% module_attribute "module_id" is_json="true" %}...

...Addgene's Data Hub"{% endraw %}{% end_module_attribute %}{% module_attribute "child_css" is_json="true" %}...%}{% raw %}{}{% endraw %}{% end_module_attribute %}{% module_attribute "css" is_json="true" %}{% raw %...padding-right":"275px"}{% endraw %}{% end_module_attribute %}{% module_attribute "definition_id" is_json="true...%}{% raw %}null{% endraw %}{% end_module_attribute %}{% module_attribute "field_types" is_json="true" ...style":"group"}{% endraw %}{% end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw... raw %}null{% endraw %}{% end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}...user_generated_content":false}{% endraw %}{% end_module_attribute %}{% module_attribute "module_id" is_json="true" %}...

...journey. Figure 2: Annual distribution of items to countries with a distributor. Arrows indicate the year...amazing 111 countries (Figure 1)! But while we were making the materials available to many countries, import...country to country.   Figure 1: Number of countries Addgene distributes to per year, 2005-2023. We identified... we identified countries that would benefit from a third-party “helper”: a distributor who, among other... India, and Mexico. Table 2: Addgene distributors Distributor Partnership Start Date Country Served...in material distribution after we partnered with them (Figure 2). In others, the distributor helped solve...that we could identify the need for distributors in some countries. ‌We’ve developed systems to allow ...

...a sequence recognized by the restriction enzyme MstII. When a restriction digest with MstII and southern...than real-time PCR (4). Restriction mapping and modern sequencing Restriction mapping informs on DNA sequence...different restriction enzyme digests. Incubating a DNA sample of unknown sequence with restriction enzymes...has changed, restriction enzymes are certainly not dead yet! Labs still keep restriction enzymes on hand...This post was contributed by guest blogger, Krissy Lyon, a PhD candidate in Neuroscience at Harvard University...three historical techniques: southern blotting, restriction mapping, and sequencing gels, as well as their...fluorescence for fluorescently labeled probes. Colorimetric and luminescence based detection methods are...

...categories: parametric and non-parametric. In this blog we will be discussing parametric tests. Parametric tests...be met for a parametric test are as follows:   Parametric test assumptions: Distribution – Normal Variables...   Fig. 2: Examples of data distribution profiles.   Parametric tests  Statistical tests fall into...derived from a theoretical distribution. If your data is normally distributed, it will form (more or less... If your data does not fit parametric assumptions, then a non-parametric test is a better option. Stay...numerical risk allowance in a statistical test. Distribution - spread of data; typically, normal (bell curve...data doesn’t fit the requirements above, a non-parametric test may be the way to go, which we will discuss...

...top ten countries for each group (Figure 4).    Figure 4: Addgene deposits and distribution by country...deposited by each country. Countries shown are top 10 depositing countries. (B) Total CRISPR plasmids ...requested by each country. Countries shown are the top 10 requesting countries. FR=France; KR=South Korea...in ~40 different countries, and we’ve shipped CRISPR plasmids to labs in ~100 countries.  The impact Addgene...repository has grown to include many CRISPR tools.  Distributing and depositing In Figure 1, you can see cumulative...cumulative numbers of CRISPR plasmid distribution and deposits (Figures 1A and 1B); the cumulative number...cumulative number of CRISPR libraries Addgene has distributed (1C), from 2012 to 2023. Addgene’s collection...

...early-phase clinical trials and have shown promising preliminary results (Dimitri et al). CRISPR engineering...editor-based clinical trial for SCD in 2022 and Editas Medicine starting a Cas12-based clinical trial for SCD in ... This ongoing trial is sponsored by Excision Biotherapeutics. Additional ongoing trials The CRISPR therapies...approved or have made significant progress in clinical trials.     Figure 1: Types of delivery and editing...of the cancer-fighting component, CAR. Clinical trials are underway to explore CRISPR’s capacity for both... returning them to the patient. The first human trial involving CRISPR Cas9 for these diseases was sponsored...and CRISPR Therapeutics. Data shared from these trials has been outstanding, with almost all patients ...

...assist with restriction enzyme digestion (usually 3-6bp) Restriction Site: Your chosen restriction site for...fusing the upstream restriction site to the forward primer and the downstream restriction site to the reverse...discussed in our restriction cloning post. However, even more than with standard restriction cloning, it is...of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that...itself are very similar to those outlined in our restriction cloning post with a few quirks specific to the...amplified (usually 18-21bp) When selecting restriction sites, you should use a DNA analysis tool, such...Sequence Analyzer, to allow you to identify which restriction sites are present in a given sequence. You want...

...Expression Vectors"{% endraw %}{% end_module_attribute %}{% module_attribute "child_css" is_json="true" %}...%}{% raw %}null{% endraw %}{% end_module_attribute %}{% module_attribute "css" is_json="true" %}{% raw...padding-right":"255px"}{% endraw %}{% end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw... raw %}null{% endraw %}{% end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}..._follow":false}{% endraw %}{% end_module_attribute %}{% module_attribute "module_id" is_json="true" %}...% raw %}8243667{% endraw %}{% end_module_attribute %}{% module_attribute "schema_version" is_json="true...true" %}{% raw %}2{% endraw %}{% end_module_attribute %}{% module_attribute "style" is_json="true" %}{% raw...

...anticipated restriction analysis results (left) and validation PCR followed by Restriction Fragment Length...This post was contributed by guest blogger, Kristian Laursen from Cornell University. Site directed mutagenesis...conventional cloning (to introduce or remove restriction sites), in mapping of regulatory elements (to...the plasmid template (blue), and introduce a restriction site “A*” (in addition to the “A” site already...in the parent vector). Also shown are the “B” restriction sites, which flank the targeted region. Upon ...amplification, the template is removed by DpnI restriction digestion leaving behind only the mutagenized...Polymorphism (RFLP) analysis (right). Introduction of restriction site “A*” results in an additional band when ...

... tools created by the Trimmer Lab that will be the first antibodies distributed by Addgene.  The plasmids... constructs. The Trimmer lab found that the recognition sequence for the restriction enzyme BciVI was ...Neuroscience AntiBody Open Resource). The antibodies distributed through NABOR are recombinant antibodies expressed...constructing the plasmids for expression of mAbs, the Trimmer lab isolated RNA from cryopreserved hybridomas,...Constructs were then verified by both diagnostic restriction digest and colony PCR.   Fig. 1: (A) ...can be a threat to future reproducibility. The Trimmer Lab found that in addition to recovering RNA from...lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation by immunofluorescence-immunocytochemistry...