We narrowed to 94 results for: Tet-on

1. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

   Type

   Blog Post

   Published

   Nov. 14, 2016, 3:30 p.m.

   ... the world from the far-eastern islands,” said Tetsushi Sakuma, a lecturer at Hiroshima University who...

2. Sleeping Beauty Awakens for Genome Engineering

   Type

   Blog Post

   Published

   June 30, 2015, 2 p.m.

   ...and one set of vectors for inducible expression (pSBtet derivatives). The constitutive and inducible expression...

3. Management for Scientists: What Makes a Good Manager Anyway?

   Type

   Blog Post

   Published

   Dec. 9, 2014, 2:22 p.m.

   ...managing people: Skills for New Managers by Morey Stettner Successful Manager's Handbook: Develop Yourself...

4. Hot Plasmids and Viral Preps - September 2021

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   Blog Post

   Published

   Sept. 21, 2021, 1:15 p.m.

   ...the expression of fluorescent proteins and oxytetracycline enzymes. Next, this system was used to recreate...

5. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

   Type

   Blog Post

   Published

   Oct. 29, 2015, 2:30 p.m.

   ...experiment. Dr. Cohen and colleagues treated a tetracycline resistant plasmid, pSC101, and a newly developed...

6. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

   Type

   Blog Post

   Published

   Sept. 3, 2019, 1:15 p.m.

   ...latter experiments, such as transcription factors tethered to dCas9, require long time scales with constitutively...

7. New and Upcoming Viral Vectors - June 2019

   Type

   Blog Post

   Published

   June 18, 2019, 1:15 p.m.

   ...  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120...

8. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   Published

   March 14, 2019, 1:54 p.m.

   ...Exosome-Associated AAV by Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development...

9. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

   Type

   Blog Post

   Published

   July 27, 2017, 1:11 p.m.

   ...hypothesized that she could make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to...

10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...sgRNA incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which...

11. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...prone to aggregation in cells compared to the tetrameric Dronpa it was derived from. What are photoswitchable...

12. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ... In corals, the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural...

13. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...orientation, allowing the four Cre proteins to form a tetramer. A double-strand DNA break occurs within the core...

14. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...You can clone your gene of interest into this tetracycline inducible, 3rd generation lentiviral vector ...

15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...

16. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...Acr proteins can also be used to prevent dCas9 tethering to DNA. Since these early anti-CRISPR studies,...

17. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 3, 2022, 1:15 p.m.

    ...in N-terminal fragment Constant rate of p53 tetramerization in response to DNA damage controls the p53 ...

18. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...reaction of HRP with its substrate, 3,3′,5,5′-Tetramethylbenzidine (TMB). The addition of hydrochloric acid...

19. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    Published

    Oct. 30, 2018, 1:04 p.m.

    ...perspective of stem cell research. Best Pract Res Clin Obstet Gynaecol, 2004. 18(6): p. 827-42. PubMed PMID: ...

20. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    ...dimerization (i.e. EGFP) [1, 2] or even form obligate tetramers (i.e. DsRed) [3]. This is a problem for fusion...