We narrowed to 107 results for: Tore
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TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....status we call “Archived.” This status allows us to store plasmids and make them available to scientists without...
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Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....transgene. One such marker is the white gene, which restores eye color to fly lines where the endogenous white... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m.... the blog originally started as a way to easily store and share in-depth answers to frequently asked questions... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....nanobody. As a proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion into mCherry or GFP nanobodies... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....addition to output files, this is also where you can store any power point, excel, or other files relevant ... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....refers to the work put into creating a permanent store of knowledge (a schema).” In scicomm, I like to... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....sample(s) are resuspended in water or TE buffer, store them in a -80°C freezer to prevent RNA degradation... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m.... some exist as dsRNA. Still others use dsDNA to store their genetic information. All of these viruses ... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....are sequencing from the exact same stock that we store and distribute from. Of course, your culture should... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....significantly higher than the same prep that has been stored in the freezer. If you plan to freeze a batch of... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....excited to see what the next few months have in store for us. If you’re thinking about making the switch... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....preparation. rAAVs Now your AAV virions are ready to store or use. They are quite stable and will withstand... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m.... linked to a chromophore and functions as a photoreceptor in the eye. There are two flavors of rhodopsin... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....-3.13. To test xCas9 3.0-3.13, Hu et al. first restored catalytic residues D10 and H840. To ensure that... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....survival (for Gateway plasmids). We also like to store plasmids that need extra stability, such as some... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....testing. An aliquot of our recombinant antibodies is stored at 37 °C for a period of two weeks. After this ... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....containing buffer, polymerase and dNTPs, can be stored frozen: just defrost and add your primers and template... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....titrating AAV by qPCR, viral DNA is amplified and monitored in real time. When the PCR is complete, the results... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....cultures of your positive clones. If you want to store your clones longer term, just streak the colonies...