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We narrowed to 109 results for: crispr library

Showing: 61 - 80 of 109 results

  1. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a... Penn State Protein Ladder Engineered miniature CRISPR-Cas system for mammalian genome regulation and ...gene perturbation by: Gabrielle Clouse While many CRISPR-based genetic screening methods have primarily ...10.1038/s41598-021-96051-x Engineered miniature CRISPR-Cas system for mammalian genome regulation and ...and editing by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion proteins, such as Cas9 and...engineering applications. CasMINI, a new miniature CRISPR-Cas system for editing and gene activation, is ...

  2. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...blog Browse our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...recent papers describe the development of CRISPR activation (CRISPRa) tools for neuroscience, including transgenic...neuroscience research. What is CRISPR-based activation? To summarize, CRISPRa uses a nuclease-dead variant...nature makes CRISPRa much simpler to implement in genome-wide screens over cDNA libraries. However, using... activator were transduced with a pooled sgRNA library and were subsequently cultured for nearly two weeks...molecular gymnastics for sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for ...

  3. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach." Nature... Out Our CRISPR Guide Pages Find Validated gRNAs for Your Experiments Get Ready-to-Use CRISPR Lentivirus...Institute for Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of.... To date his group has develop several popular CRISPR-based tools for altering DNA sequence or regulating...PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for genome editing and cancer... Alex Chavez Learn about Sequencing Options for CRISPR Genotyping Cas9 Activators: A Practical Guide ... crispr...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook More ...the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9 The Other...as clonal differences. If you are performing CRISPR library screens, the same principle holds true - the...for unintended consequences in an experiment. For CRISPR work, this often means DNA cleavage at unanticipated...will discuss when off-targets can be an issue in CRISPR, how to predict and minimize them, and ways to ...t be avoided. What are off-target effects? The CRISPR-Cas system is an RNA-guided nuclease that either...sequence similarity to the on-target site. When a CRISPR-Cas system localizes to unintended sites and performs...

  5. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ... test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with... certain model organisms. In mammals, most CRISPR libraries are assembled and delivered virally, but these...etc. MoClo is especially useful for more complex CRISPR experiments (e.g., many guides/parts delivered)...

  6. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some ...Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website Visit our CRISPR Guide Pages...editors, Casilio, CombiGEM, and a variety of pooled libraries. Not all of these technologies were without controversy...requested plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab. It's name,.... Cas9, of course, is the key component of most CRISPR systems and generates double strange breaks at ...Ran, F. Ann, et al. "Genome engineering using the CRISPR-Cas9 system." Nature protocols 8.11 (2013): 2281... Pages Browse Our CRISPR Collection Find gRNA Design Tools ...

  8. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...be saved to a Semantic Scholar library. You can add hashtags to library entries, which can help you organize...papers Papers are easily saved to a Meta library. The library will suggest additional papers based on ...including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There isn’t technically...on the papers you have saved. This library can also be synced with a Mendeley reference manager account...

  9. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...  New to CRISPR/Cas9 Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale... awards that will be bestowed upon discovers of CRISPR/Cas9 genome engineering, Dr. Jennifer Doudna and...Addgene Scientist Matt Ferenc (one of our resident CRISPR experts) at the 2014 Johnson & Johnson’s Dr. Paul...dinner which took place at the New York Public library. Being in the presence of scientific heroines is...disease. They are finding they have been able to use CRISPR/Cas9 to generate mice with genes knocked out in...technologies. Addgene is proud to be helping get CRISPR reagents out to academic labs. It is our hope that.... He offered the great analogy of comparing the CRISPR/Cas9 system to Norton Antivirus. Antivirus programs...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Your gRNA for CRISPR Genome Editing PRIME Editing: Adding Precision and Flexibility to CRISPR Editing Resources...Resources on Addgene.org CRISPR: PRIME Edit Collection The CRISPR Guide Empty gRNA Expression Vectors...prime editing efficiencies in high-throughput libraries My labmates and I wanted to find a way to predict...high-throughput ‘self-targeting’ screens with oligo libraries where pegRNAs are coupled to their target allowing...

  11. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...sequence viewer software and extensive feature library, our updated plasmid and sequence displays are ...features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies a variety...in-house software. In fact, SnapGene’s feature library includes nearly 3x as many features as could be...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display...

  12. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...available in the next few weeks. CRISPRi pooled library The Dolcetto pooled library is now available as a lentiviral...lentiviral prep. It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used...

  13. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...Lab Browse the CRISPR Collection Find CRISPR Pooled Libraries  ...question on everyone’s minds is: could NgAgo replace CRISPR? Such a drastic shift won’t happen overnight, but...are a few reasons why you might choose NgAgo over CRISPR proteins Cas9 or Cpf1 - keep reading to learn more...targeting flexibility Unlike Cas9 and the other CRISPR enzymes, NgAgo does not require a PAM sequence,...viral vectors such as AAV. Base removal RNA-guided CRISPR enzymes cause double-stranded breaks, with either... off-targets More research is needed to compare CRISPR and NgAgo, but it seems that NgAgo has lower potential... crispr...

  14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...Resources on addgene.org Addgene's CRISPR Guide Addgene's CRISPR 101 eBook CRISPR resources More resources on... DNA, CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) are powerful tools. CRISPRi uses a... for us, CRISPR is an incredibly versatile system that can be tweaked to target ncRNA. CRISPR/Cas9 techniques...Bester 2021.    Alternative CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely...If the CRISPR/Cas9 system is not effective for your experiments, there are plenty of other CRISPR-based...many protein-coding genes thanks to systems like CRISPR/Cas9 that allow them to manipulate full genes and... Knock-out: Targeting short vs. long ncRNAs The CRISPR/Cas9 system is often used to knock-out, or permanently...

  15. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...Modifying the CRISPRi system for multidirectional control The CRISPR interference (CRISPRi) model consists...Addgene: Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described...Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds." Adam has also created a video to...metabolic biofactories by demonstrating the use of CRISPR-based RNA scaffolds to mimic natural transcriptional...AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific...Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell, 160(1), 339-350 (2015). PubMed...browse/article/6441/ Resources: An open source library of characterized aptamers and supporting publication...

  16. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...Stegmeier deposited the ClonTracer Barcoding Library, a library that “enables the high-resolution tracking...Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function...

  17. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like to knockout a gene,...Service, we’re distributing lentivirus (with many CRISPR tools included among the preps that are currently...lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral... in experiments other than just those involving CRISPR and so we also have lentivirus for delivering control...

  18. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...morning, John Doench’s opening presentation at the CRISPR screens symposia outlined the fantastic work this... Institute lab is doing creating well-optimized CRISPR screening technology. Addgene got several shout-outs...shout-outs for making popular screening libraries like Brie and Brunello available to the scientific community...Use Addgene's Microbiology Pages Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn ...

  19. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... TALEN kit to CRISPR/Cas9? When would you choose one or the other? Musunuru: The CRISPR/Cas9 system is...system for CRISPR/Cas9 (also available from Addgene) only has two plasmids. In many cases, CRISPR may be ... now makes possible, and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar with...15 base pairs is take four plasmids out of the library in the appropriate combination and do a restriction...be more efficient. You might often start with CRISPR/Cas9, but TALENs do have more flexibility in terms...data to date suggest TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN ...

  20. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ....02364-19 More CRISPR Blog Posts from Addgene: CRISPR 101: Homology Directed Repair CRISPR Protocol for...Deletions in Mammalian Cell Lines [Video] Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale ... UNC at Chapel Hill. One advantage to using the CRISPR/Cas system for genome engineering is the fact that...NHEJ is consequently the principal means by which CRISPR/Cas9-introduced breaks are repaired. The following...variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of hundreds of base pairs... Y, Zhang F (2013) Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell...S-S, Ding X-Y, Guo X-P, Jin Q, Sun Y-C (2020) A CRISPR-Assisted Nonhomologous End-Joining Strategy for...
Showing: 61 - 80 of 109 results