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We narrowed to 294 results for: expression vector

Showing: 61 - 80 of 294 results

  1. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...elements for vector propagation. This post, along with a future companion post on mammalian vectors, will catch...typically found as a single copy. Unlike the ARS vectors, CEN vectors are stable without integration. Yeast Episomal...of marker gene expression is to use a partially defective promoter to drive expression of the selection...plasmid maintenance within an E. coli cell, but vectors can be widely utilized across many different cell...core replication and resistance features of yeast vectors and explain how they differ from the bacterial ...previously described. Why do scientists use yeast vectors? Yeast are eukaryotes and thus contain complex ...facilitate simple gene replacement/mutation. Yeast expression plasmids used in the lab typically contain all...

  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ... SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1...-miR30 (CMV-TO) expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9...a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...enhancing target gene expression anywhere from 87x to 20,000x over endogenous expression (depending on the...

  3. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...to reduce basal expression levels; expression vector cannot have p15A origin of replication F- ompT lon...) pLysE has higher T7 lysozyme expression than pLysS; expression vector cannot have p15A origin of replication...Rhamnose-tunable T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have p15A origin...for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein in ...to avoid them. Why do I need an expression strain? Protein expression from high-copy number plasmids and...Addgene's curated list of Bacterial Expression Systems Table 1: E. coli expression strains  Note: All strains ...General protein expression BL21 (DE3) pLysS* Chloramphenicol (pLysS) pLysS expresses T7 lysozyme to ...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...protein expression constructs fused with epitopes or fluorophores, generate expression vectors with various...AAV transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila...parasites that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides ... HEK293FT cells expressing a single gRNA targeting a single gene. The gRNA expressing cells were then ... to test multiple expression systems before they achieve the appropriate expression level for their gene...various promoters suitable for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic... lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from a Drosophila U6:2 ...

  5. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...cerevisiae expression vectors are available at Addgene. A set of mammalian expression vectors containing... be easily expressed in non-mammalian cells. Yeast expression plasmids The yeast expression constructs....  Bacterial expression plasmids For those constructs to be used in bacterial expression systems, Ginkgo...backbones which drive expression using either TEF1 or GAL1 promoters. These vectors are intended for the...tag, cleavage, codon usage, and expression system. Mammalian expression plasmids Finally a set of wild ... (spike protein) cloned into a CMV driven expression vector are also available. These contain no tags ...coding genes in E. coli and S. cerevisiae expression vectors in late March. The first set of 383 constructs...

  6. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...Expanding the viral vector knowledge base: You can help! We’ve been expanding our viral vector educational resources...Beginners Lentiviral Vector Uses and Overview AAV: A Versatile Tool for Gene Expression in Mammals Additional...spending time and money producing virus from select vectors in the repository yourself, you can now order ready-to-use... production process while still ordering viral vectors. Virus production can be a frustrating process ...Lentivirus As some of the most popular lentiviral vectors in the repository are used to produce CRISPR tools... to knockout a gene, replace it, modulate its expression, or perform a genome-wide screen, we’ve got viruses...experiments, while rtTA can be used to activate expression of a gene under the control of the tet operator...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...recall, are the outer shell of an AAV vector, which determine the vector’s tropism, including its ability to...be paired with Cre-expressing transgenic mouse lines to restrict transgene expression to specific tissues...all take up precious cargo space in AAV vectors. Dual expression systems, which use two or more AAVs to...payloads. Since most systemic vectors are primarily derived from AAV9, the vectors have to be administered ...on the Addgene blog Viral Vectors 101: AAV Variables That Matter Viral Vectors 101: An Introduction to ...Selection of an Efficient AAV Vector for Robust CNS Transgene Expression. Molecular Therapy - Methods ...be suitable for your experiment. Like all viral vectors, the suitability of a systemic capsid for an individual...

  8. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ... of expression. For example, CAG is a strong promoter that drives very high, ubiquitous expression, while...outside of the ITRs does not get packaged in the AAV vector. The cloning capacity between the ITRs is ~4.4 ...of interest. Promoter The promoter drives the expression of the AAV transgene. On a plasmid map, promoters...type-specific, which can help you restrict the expression of your transgene to a specific species or location...to spatially and temporally control transgene expression. Lox sites are directional 34 bp sequences that...nuclear export of mRNA and helps increase the expression of your transgene. The WPRE is located at the...design a construct for the successful and specific expression of your gene of interest. References Haery ...

  9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...that have excised the vector backbone. Vectors pTOX4, pTOX5, and pTOX6 express the YhaV, MqsR, and Tse2...excision of the vector backbone. To do this, rhamnose is added which induces expression of the toxin. Any...encode the amilCP chromoprotein. Vectors pTOX7, pTOX8, and pTOX9 express the YhaV, MqsR, and Tse2 toxins...gfasPurple) (Figure 2). Figure 2: Chromoprotein expression from bacteria on agar plate. Image: Liljeruhm...intensities, maturation times, and fitness cost of expression. They found that the cellular fitness cost varied...lost color overnight in liquid culture, though expressing the chromoproteins from the chromosome could ...allelic exchange Figure 3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus et ...

  10. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...Vital component for expression vectors: determines which cell types the gene is expressed in and amount of... referred to as “vectors” or “constructs.” To insert a gene of interest into a vector, scientists may ...plasmid with its elements described below.   Vector Element Description Origin of Replication (ORI...sites allowing for the easy insertion of DNA. In expression plasmids, the MCS is often downstream from a ...Regardless, once the cloning steps are complete, the vector containing the newly inserted gene is transformed...Generally, scientists use plasmids to manipulate gene expression in target cells. Characteristics such as flexibility...common plasmid types include cloning plasmids, expression plasmids, gene knock-down plasmids, reporter ...

  11. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ... integrate into the host genome or temporarily express whatever it encodes. This ability to infect cells... Viruses are essentially DNA (or RNA) delivery vectors, so what makes them better than the alternatives...measures in a separate, upcoming post. If a viral vector seems like the right approach for your experiment...are generally used to achieve transient, high expression of the relevant cargo without the fear of random...Virus Pros Cons Retrovirus Stable Gene Expression Broad Tropism Only Infects Dividing Cells...Little Risk of Insertional Mutagenesis) High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity...

  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...counterselection vector is the pDS132 sacB-based allelic exchange vector. This vector contains sacB, an...Tight control of toxin expression is absolutely necessary, because leaky expression of toxin could select...introduce a mutation in the chromosome is to disrupt expression of a gene with an antibiotic resistance cassette...in the chromosome, sometimes interfering with expression of surrounding genes. However, there are ways...selecting for an antibiotic resistance marker from the vector (abR, Figure 1). The resolution step (“double crossover...leads to the desired mutation and excision of the vector backbone. This step requires “counter-selection...colonies isolated are those where the allelic exchange vector has undergone single crossover and integrated into...

  13. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...About Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional...often employed to establish cell lines that stably express a gene of interest. While the general approach ...quiescent cells do not take up nucleic acid or express transgenes as well as actively dividing cells resulting...compete with host cells for nutrients and can alter expression of receptors, ion channels, and growth factors...on a variety of factors such as the transgene expressed, the viral storage buffers used, and other experimental... by the envelop protein. Many times lentiviral vectors are engineered with non-native envelopes, a process...will be able to overcome differences in receptor expression. It's all in the mix Even before the lentiviral...

  14. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...engineered into viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the ...analyte), and accumulation of ATP activated GFP expression (receptor), then the transducer could be a microscope...retrovirus, can either permanently integrate and/or express long term (up to several years!). Biosensors tend...biosensor to specific tissues or cell (sub)types for expression and downstream monitoring. Nervous about making...Biosensors Viral Service: Biosensor AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview... as stable systems, rather than transiently overexpressing at the time of use. Another great viral perk...

  15. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...the 34 new preps available in the Addgene viral vector repository! This includes the first Addgene prep...X1.1 serotype.  In rodents, AAV9-X1.1 enables expression in brain endothelial cells via an injection into...actually targets neurons, and has 45-fold higher expression levels compared to AAV9. You can now target these... AAV9-X1.1!  We also have eight new AAV-PHP.eB vectors from the Jonathan Ting lab at the Allen Institute...BRAIN Armamentarium Collection. These preps drive expression of either Cre recombinase or a fluorescent marker...carried out before. We have a bunch of other new AAV vectors as well — including next-generation biosensors ...Check them out! Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category...

  16. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...communication. Viral vectors and the nervous system With the advent of viral vector technology, new methods... at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have been widely used...transgene expression. Fluorescent proteins are widely used for tracing and their expression is often made...Cre-dependent. If Cre-dependent vectors are used in transgenic animals that express Cre in only specific cell...of infected cells that also express Cre. Another advantage of AAV vectors is that there are well-established...Kohara et al. 2014 traced neurons with an AAV9 vector expressing a Cre-dependent fluorescent marker. Injection...genome is a single-stranded DNA molecule and AAV vectors are replication deficient (i.e. they cannot easily...

  17. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...plasmids and expressed via cells cultured with the vectors. This allows the viral vectors to reproduce...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...viral particle is called a virion. Finally, viral vectors, which are used in the lab, are distinct from viruses...copies itself and “splits” into two cells. Viral vectors Viruses are quite appealing as molecular biology...

  18. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ... tool for gene expression in mammals. Resources on Addgene.org Search for AAV vectors to use with your...pathogens to the CNS, but this also stops viral vectors, such as adeno-associated virus (AAV), from reaching...injection into the bloodstream, but requires a high vector dose and is unable to reach all regions of the ...phenotypic effects such as those that affected gene expression, mRNA splicing, or protein coding regions. In...one, Ly6a, showed differential protein and RNA expression between the brains of C57BL/6J mice and BALB/...strong linkage to the PHP.B phenotype and its expression pattern in the mouse brain.  Both labs then took...Deverman lab started by seeing how changes in LY6A’s expression affected PHP.B’s transduction rates. Knock out...

  19. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...populations. We now offer a Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase...AAV1 pAAV-ihSyn1-DIO-tTA Viral vectors coming soon! These vectors listed below should be released at... of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the...detection PSAM4 GlyR for chemogenetic inhibition Flp expression for intersectional studies And more! Control...Recombinase AAV Finally, we are excited to add Flp-expression AAV to our recombinase AAV inventory. Flp is ...AAV pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors New optogenetics inhibitors Plasmid Serotype...

  20. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from ...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...this for doxycycline inducible (tet-off) gene expression! We've also added AAV1 and AAV5 preparations ... precision.   Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category... multiple serotypes Looking for more viral vectors? Browse the full viral services collection or check... check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the...below to keep up with the latest in Addgene's viral vector services and resources!   This blog post was written...
Showing: 61 - 80 of 294 results