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We narrowed to 92 results for: lenti crispr plasmid

Showing: 61 - 80 of 92 results

  1. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...And when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs....org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion for CRISPR online course...frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan, and Benjie...nonprofit that would facilitate the sharing of plasmids between scientists. The service would allow people...Addgene before we were a “labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP... our first major repository milestone — 10,000 plasmids deposited and available — and nearly seven before...possible for scientists to both deposit and request plasmids, was a major focus of our first decade. We did...

  2. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...View Our CRISPR Guide Pages Find CRISPR Plasmids for Your Experiments Get Ready-to-Use CRISPR Lentivirus...steps in the CRISPR experimental process is validating edits. Regardless of which CRISPR genome editing...discussed in CRISPR 101: Validating Your Genome Edit, there are a variety of options for CRISPR genotyping...Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9 Additional Resources on ...off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors." Nature biotechnology...target gene. The validation process, also known as CRISPR genotyping, is critical to demonstrating causal...biology. It is key to address these concerns as CRISPR use grows across the life sciences and to establish...

  3. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...Addgene's CRISPR resources Request ready-to-use viral preps of select CRISPR lentiviral plasmids Additional...design your CRISPR gRNA CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...sequence for CRISPR-Cas9 targeting is led by a ~20 bp gRNA which is part of the larger CRISPR RNA (crRNA...surprise then that stability of the RNA used in CRISPR-Cas9 experiments – the “gRNA” – suffers from the...modifications listed above were developed before CRISPR-Cas9, for use in applications like siRNA and antisense...to find pre-tested gRNA sequences for your next CRISPR experiment.    Chemical RNA modifications... On-off switches for gRNAs A major drawback of CRISPR-Cas9 technology is that most expression systems...

  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...comparison of an shRNA plasmid sequence and an miRNA plasmid sequence. (A) Plasmid sequence from pLVUHshp53....   Expressing shRNAs using plasmids The design of shRNA plasmids requires three important steps: ...between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence...shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is meant...Tools for an Old Favorite CRISPR 101: Targeting RNA with Cas13a (C2c2) Plasmids 101: The Promoter Region... to. When it comes to altering gene expression, CRISPR technologies have gradually taken over as the preferred...1990s and quickly rose to popularity. Compared to CRISPR, RNAi methods are often quicker and the repositories...

  5. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...Culture Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial... that made it easy to share plasmids, both for people depositing plasmids and for people requesting them...Expression Systems AAV Plasmids Blogs Origin of Replication What is a Plasmid? How to Design Your...Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to Verify Your Plasmid Using a Restriction...video, however, is “What is a Plasmid?”, An animated explainer of a plasmid and its parts that helps the...Resource Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus...to help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols...

  6. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...Addgene Blog First time CRISPR user? Check out our tips for 1st time CRISPR users Learn how to Choose...Here Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page ...resolution, the Xu lab created a dCas9-based CAPTURE (CRISPR Affinity Purification in situ of Regulatory Elements...at hundreds of loci at once (Liu et al., 2020). CRISPR Affinity Purification in situ of Regulatory Elements...CAPTURE 2.0 to be used with primary cells. Find plasmids for CAPTURE 2.0! When compared to the original... crispr...from three to two. Lentiviral delivery of BioTAP-tagged dCas9 and gRNAs. Using lentiviral delivery of the...

  7. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...on Your CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled ...How to Conduct Genome-Wide CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources on Addgene.org...not quite as broad as for knockout via CRISPR cutting. For CRISPRa, it is most-efficacious to target a ~... Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to...considerations before you start an experiment with CRISPR The hammer, the jigsaw, and the wrench are all ...the same is true when designing gRNAs for using CRISPR technology – the “best” gRNA depends an awful lot...hammer: Gene knockout by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated...

  8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    ...Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the immune system and ...development in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering...contrast to using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering...analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited... immunology resources. If you have a great new plasmid you’d like to share with the scientific community... et al. "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643...

  9. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that....cell.2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and transcriptional activation screening...Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction Additional... individually overexpressed and available for lentiviral packaging and delivery to target cells as a pooled... Fig. 1: Barcoded TF library was packaged in lentiviral particles and used to transduce human embryonic...

  10. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...mesothelioma tumorigenesis. They plan to use CRISPR and lentiviral plasmids from Addgene to knockout, knockdown...of these genes, CRISPR to disrupt their function, and C. elegans overexpression plasmids to upregulate ... disease. This year, Addgene will be providing plasmid grants worth $2,000 each to two labs through the...the BeHEARD Award. Read on to learn how plasmids from Addgene will be used to accelerate rare disease ...in C. elegans, Dr. Di Schiavi will use Addgene plasmids to characterize smn1 interactions with other genes... Support Rare Disease Research: IFOPA Donating Plasmids to Researchers in Developing Countries with Seeding...Seeding Labs Resources on Addgene.org Find Plasmids for Disease Research ...

  11. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...Addgene Plasmids Searchable and Sortable gRNAs for Your Next CRISPR Experiment Suggest A Plasmid You'd ... such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain. For plasmids containing...deposits with more than 10 plasmids is to use our deposit spreadsheet?  Our plasmid deposit process can be... Send plasmid information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene...spreadsheet and copy and paste your plasmid data directly into our file, one plasmid per row, as outlined by the... Data Field Description Plasmid Name Use a descriptive plasmid name and match the name as it ...application of the plasmid and will help a scientist outside your field understand if this plasmid will work for...

  12. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...envelope and packaging plasmids as well as transfer plasmids. Download the orange lentivirus background.     ...Otherwise, check out our plasmid and DNA backgrounds. Download the blue plasmid background.     You can... the plasmids that Addgene offers by visiting our collections page. Here, you can browse plasmids by field... and lentiviral preps! Download the green AAV background.       Some of our lentiviral plasmids include... have ready-to-use lentiviral preps for COVID-19 research, pooled CRISPR lentiirus libraries, pooled barcoding...your favorite reagent, scientific technique, or plasmid repository at your next meeting.     If you’re...cute mascots!  Blugene has been helping us make Plasmids 101 videos and needed a break so they're hanging...

  13. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...contraction defects. The lab also plans to use CRISPR plasmids to generate deletions of candidate genes in... with the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...highlight their research and how they will use plasmids and viral preps from Addgene to further their ...BeHeard award, the ACD will use Addgene’s RESOLUTE plasmid collection to perform a functional screen in mammalian...therapeutic development,” says Iyer. Since the RESOLUTE plasmid collection consists of human and murine solute ...happening within the keratinocytes. “We hope to use CRISPR libraries from Addgene to set up assays to see ...research questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building...

  14. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...move antibiotic resistance genes between plasmids or from plasmids to the bacteria’s genome. In humans, if...encodes a Type I-F CRISPR-Cas system. This system has four major components: 1) a CRISPR RNA (crRNA), 2) ...activity (Voigt et al., 2016). Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise...codon-optimized wild-type piggyBac transposase.  piggyBac plasmids    Tol2  Tol2 was the first reported active DNA...increase in transposition activity  Tol2 transposon plasmids    Applications of transposons Now that you’ve...sets the stage for combining the super powers of CRISPR and transposons to allow for the targeted delivery... (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin...

  15. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ... Tracing Read How CRISPR Can Be Used in Genome-Wide Screening Browse 28 Hot Plasmid Technologies from ...for Your Research View our Hot Plasmid Pages to See the Latest Plasmid Technologies ... drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As described in...It’s also possible you could make use of these plasmids in living animals, to explore pathways that confer... from other researchers, and cloned them into lentiviral vectors to generate a kit which is now available... cloned all the cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode ...

  16. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...our control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for expression...  New lentiviral preps SARS-CoV-2 protein expression The Krogan lab quickly shared plasmids expressing...we've turned some of the most requested plasmids into lentiviral preps to speed up COVID-19 research even...It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used for genome-wide...Testing AAV inventory for the entire collection! Plasmid Serotype Name Depositor 27056 AAVrg pAAV-Ef1a-DIO...constructs that allow for targeted expression. Plasmid 75470 is now available in AAV1 for Flp-dependent... mCherry reporter. We now also offer AAVrg for plasmid 83898, which targets expression of ChR2 to forebrain...

  17. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...Discuss Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ...

  18. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular ...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1... may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the different...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...

  19. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...18,359 viral items, and 166,247 plasmids. We welcomed 18,899 plasmids, deposited from 1,106 labs in 504...transcription factor library, in lentiviral vectors. At 3500 plasmids, this represents the largest single...September, we released the fourth edition of our Plasmids 101 eBook. This updated version of one of our ...the recent release of the Spanish version of our CRISPR 101 eBook and the upcoming release of the second...to announce that we are now able to ship rabies plasmids in our repository to international requestors....

  20. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...write about a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast!  MarathonRT... DNA sequence in only 1 min. Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused...’s lab at Yale University recently deposited a plasmid for expressing a novel reverse transcriptase (RT...polymerase processivity. Please find the MarathonRT plasmid here! Zhao C, et al. RNA. 2018 PubMed PMID:29109157... cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody fused with...
Showing: 61 - 80 of 92 results