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In Category

We narrowed to 144 results for: lentiviral

Showing: 61 - 80 of 144 results

  1. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...flexibility. Find the engineered prime editors here A lentiviral CRISPR knockout library that targets human epigenetic...the scale of your CRISPR screens by using this lentiviral CRISPR human genome-wide library deposited by...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...depression. The researchers found that injecting lentiviral vectors expressing BDNF in the hippocampus causes...Chandrasekar, and Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of microRNA124a in the hippocampus...floating. In the same paper, the authors used lentivirus to introduce an shRNA to downregulate expression...

  5. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...by the Day Lab, who developed neuron-optimized lentiviral vectors for in vitro and in vivo CRISPRa (Savell...neuronal physiology. The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV...Savell et al., 2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...

  8. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...components to reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for in vivo and in...cytoplasm and can be fluorescently labeled. Using lentiviral vectors to stably or inducibly express tagged... to target multiple delivery systems, such as lentivirus and MMLV gag, to specific cell types. It can ...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Multiple Lentiviral Expression Systems (MuLE) Kit: This kit can be used to create lentiviral vectors expressing...CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014)...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...categories (e.g., for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression...be prone to recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the...

  15. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ... with HIV-1. For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy...PubMed Central PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at...people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral...

  18. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...to create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed... MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with just the...collection of plasmids comprising the multiple lentiviral expression (MuLE) system from the laboratory ... combinations of genetic elements in a single lentiviral vector provides a powerful tool to modify mammalian...routes in various conditions. The plasmids and lentiviral vectors encoding these proteins can also be used...the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series of building-block...

  19. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...CRISPR-Cpf1(Cas12a) Double Knockout Library is a lentiviral library targeting 26 genes chosen from an analysis...
Showing: 61 - 80 of 144 results