We narrowed to 99 results for: mouse c rel

...genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated from mouse fibroblasts in 2006 in Shinya...Yamanaka, Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined...for Disease and Open Science: An Interview with Darrell Kotton Four Factors that Differentiate the Stem...

...AAV2 serotypes in order to create an atlas of the mouse brain. This atlas enables researchers to quickly...recently, OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun Tag (Viswanathan, 2015). The principal...pdb-top63. PubMed PMID: 20150100. 4. Shaner, Nathan C., George H. Patterson, and Michael W. Davidson. "Advances...experiments with tag-specific antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis...of epitope tags for IHC is the availability of reliable primary antibodies for their detection, particularly...cross-react with other antibodies, or are unavailable entirely. The other advantage is that these tags are small...

...fourth-generation base editors, BE4, reduce the undesired C→G or C→A conversions that can happen with BE3s. These...Compared to BE3, BE4 offers a 2.3 fold decrease in C→G and C→A products as well as a 2.3 fold decrease in indel... Hong, M., Zhang, D., Luan, C., Zhang, S., Wu, H., Gao, H., Bai, S., Li, C., Ding, R., Xue, N., Lei, Z... W., Levy, J. M., Yeh, W.-H., Zheng, C., Newby, G. A., Wilson, C., Bhaumik, M., Shubina-Oleinik, O., Holt...non-homologous end joining (NHEJ), HDR occurs at a relatively low frequency, especially in nondividing cells...particles (RNPs) to both zebrafish embryos and the mouse inner ear. Fourth-generation cytosine base editors...) and adenine base editors (ABEs). CBEs mediate a C to T change (or a G to A change on the opposite strand...

...have historically been used extensively in fly, mouse, and yeast models. Why traps are useful Traditional.... DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al., Unifying the known and unknown microbial...traps? Traps – gene, enhancer, and promoter – all rely on the random integration of a reporter construct... effective tools but neglect some biologically relevant situations. Tissue-specific genes – the genes...

...Vries (1896).  In 1928, Frederick Griffith used mouse models to prove that Streptococcus pneumoniae bacteria...rise of literary giants such as Isaac Asimov, Arthur C. Clarke, James Blish, and John W. Campbell. This period...combination of the four bases of DNA – A, T, G, and C. In this movie Ethan Hawke’s character revolts against...editors (CBE) use a cytidine deaminase to introduce a C→T change at precise locations in the genome. This ...JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR (2019) Search-and-replace...Correction: Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature 559:E8...420–424 . https://doi.org/10.1038/nature17946 Lenay C (2000) Hugo De Vries: from the theory of intracellular...

...particles or relatively short expression times, where as others, such as hSynapsin, are relatively weak and... in the Mouse Brain. PLoS ONE 8:e76310. https://doi.org/10.1371/journal.pone.0076310 Burger C, Gorbatyuk...this study were packaged at Addgene and had a relatively low recombination rate (0.1% and 0.13% respectively...neuronal phenotype in a mixed population (Burger, C. 2004, Aschauer, DF. 2013, O’Carroll, SJ. 2021). This... require gene expression in a specific cell type rely on transgenic models that express recombinases like...sites and the open reading frame is inverted in relation to the promoter, hence the name, double-floxed...homologous pairs, and that the degree of homology is correlated with the level of spontaneous recombination. ...

...Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han Y-C, Ogrodowski P, Crippa A, Rekhtman N, de...Somatic Genome Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses... Journal of Controlled Release 298:128–141 . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, Mou...efficient introduction of DNA into therapeutically relevant, non-transformed mammalian cells. These viral ... could be realized via simple switching of the relevant gRNAs and donor DNA Adenoviral CRISPR/Cas9 genome...

...gene controlling pancreatic differentiation) in mouse embryos (Miyatsuka et al.) Distinguish between models...predictable kinetics over 14 hours of expression in C. elegans embryos. However, this initial timer was ...timer could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus embryos. Of course, timers...of protein concentration. Calibration curves correlating this ratio to the total expression time can therefore...used to: Monitor dynamics of vesicle fusion and release from the plasma membrane by fusing various vesicle... situation where one wants to understand the relationship between the age of a cell, protein, or cellular...nascent tissues and the formation of limbs are correlated with changes in gene expression. In a developing...

...on cleared whole-mount specimens, such as whole mouse brains expressing green fluorescent protein (GFP... a AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract...Deisseroth. (2014) Nat Protoc., 9, 1682-1697. Pubmed. C. Soderblom, D.-H. Lee, A. Dawood, M. Carballosa, et...major advances in light microscopy, particularly related to the neurosciences.  These developments in microscopy...microscopy) (16).  Both 3DISCO and CLARITY have relied on the use of transgenic mice in which a subpopulation... our understanding of the structure-function relationship of the central nervous system under normal and...

... tool is available for human and mouse genomes only and deals with C-terminus epitope tagging. If you ...Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S et al. 2017. ...your crRNAs using other web-based design tools (for C-terminus tagging, pick a crRNA that cuts 8-15 bp downstream...insertion site (within ~8-15 bp from the stop codon for C-terminus tagging) GC content of the crRNA (aim for... genotyping can tell if HDR completely failed. If C-terminus tagging potentially disrupts protein function... O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki M et al. 2018. An efficient and scalable...epitope-tagged clonal cell lines remains a challenge as it relies on inefficient homology-directed repair (HDR) to...

...and T7E1 have been widely adopted due to their relative simplicity and low cost. The problem with these... detection rates mean the investigator can be relatively certain that their samples don’t have off-target...Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9 Additional Resources on...PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy for... 22963480. PubMed Central. 5. Tsiatis, Athanasios C., et al. "Comparison of Sanger sequencing, pyrosequencing...genotyping, is critical to demonstrating causal relationships between genotype and assayed phenotype. Verifying... for validating edits.  Mismatch cleavage assays rely on pairing between the edited strand and wild-type...

...signaling dynamics are influenced by the relatively long life-cycle of a protein from expression to degradation...and reversible gene silencing in RNAi transgenic mouse models and embryonic stem (ES) cells. These vectors... the lab established a dual-color RNAi “sensor” mouse strain in which Cre expression causes the induction...the location and distribution of synapses in the mouse brain. Kim et al is utilizing a mammalian GRASP ...the mitochondrial targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported...pCoofy series of plasmids contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep, CBP...N-terminal tag, coding sequence of the gene of interest, C-terminal tag, plant terminator, and plant resistance...

...between 1,000 nm and 1,200 nm, where there is relatively little tissue absorption, weak tissue scattering...iteration of this system called Brainbow, the Ubow mouse strain has been created to fate map Langerhans cells...new constructs have facilitated the creation of mouse lines that can be used in fate mapping experiments...system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals an essential role...understanding of pathogen-immune cell interaction relied on in vitro studies where it was difficult to apprehend...experiments, experience has shown that some are more reliable and more efficient at tracking cells. Good two-photon...

...high Cas9 MOI considering that A549 cells are relatively easy to transduce. I ended up with too many Cas9...on genomic DNA. The surveyor assay itself was relatively easy. Figure 4: Detection of edited BRAF...Once - Multiplex Expression of gRNAs Generating Mouse Models Using CRISPR/Cas9 Resources at Addgene.org... new scientific observations. References 1. Rago C, Vogelstein B, Bunz F. Genetic knockouts and knockins...Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat ...

...transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain. PLoS One. 2013 Sep 27;8(9):e76310. PubMed...Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth...CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR...mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan Roth’...

... These antibodies are recombinant versions of mouse monoclonal antibodies generated and characterized...specificity of GFP expression for the indicated ssAAV5s. C) Quantifications showing high microglia transduction... microglia-targeting AAV plasmids here! Serrano, C., et al. (2023). Simple and Highly Specific Targeting...fast-spiking neurons. The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar...dCas9-VPR, prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various...

... 79628 (C) Cv (capsid) N and C 79633 (N) and 79641 (C) NS1 (var: W98G) N and C 79635 NS3 C 79637...79637 NS2B C 79636 NS4A N 79640 NS4B C 79639 NS5 C 79632 (N) and 79631 (C) PrM N and C   Detecting...environment and relies on the fact that enhancers function independently of their position relative to a transcription...parts involved in altering gene expression are relatively well known, particularly in bacteria, the process...strains (5). MutL is highly conserved in distant relatives of E. coli so it can be used in a broad range ...The key to the biosensor lies in pH: DsRed is relatively pH-insensitive, while SEP fluoresces at cellular... and normalized to assess interactions. Higher relative firefly luciferase activity in the Co-IP compared...

...Generation of Humanized Single-chain Fv Derived from Mouse Hybridoma for Potential Targeting Application. Monoclonal...solubility (Monnier et al., 2013). The linker keeps the C-terminus of one variable domain and the N-terminus...10.3390/antib2020193  Khantasup K, Chantima W, Sangma C, Poomputsa K, Dharakul T (2015) Design and Generation...to avoid animal use by using semi-synthetic or entirely synthetic VL and VH sequences. Phage display also...scFvs are also used for eye diseases, such as age-related macular degeneration (AMD) since direction injection...of the CAR facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR... CAR has an scFv that binds cancer-related antigens, binding of the scFv part of the receptor can activate...

...Currently, Addgene carries CRISPR libraries that target mouse, human, fly, E. coli, and T. gondii genes.  2) ... Cas9 to generate the mutant cells, respectively (C). Mutant cells are screened (D) and "hits" are identified... for which the underlying genetic cause is not entirely known. Identifying which genes are important for...

... Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal (Cas9(C)-2xNLS) fragments facilitates target DNA ...activation, the c-terminal cas9 fragment was fused with FK 506 binding protein 12 (Cas9(C)-FKBP-2xNLS) and... enzymes were chosen because they cut human and mouse coding regions infrequently; any MluI, XhoI or SalI...different organelles, a cell cycle indicator for mouse embryonic stem cells and a series of inducible promoter...out there (and particularly CRISPR), it is now relatively easy to design constructs and repair templates...an n-terminal DNA recognition domain and a mostly c-terminal nuclease domain. Feng Zhang's group utilized...Treatment with rapamycin induces Cas9(N)-FRB-NES and Cas9(C)-FKBP-2xNLS dimerization and net influx into the nucleus...