We narrowed to 701 results for: plasmid

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

March 8, 2017, 3:15 p.m.

In our new Hot Plasmids segment on the Addgene podcast, you can quickly learn about a variety of new plasmid technologies from our quarterly newsletter.

May 17, 2017, 3:37 p.m.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Tips to help you save time when using the the Basic Local Alignment Search Tool (BLAST) provided by NCBI to compare sequence results.

May 29, 2014, 1:29 p.m.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Sept. 14, 2016, 2:30 p.m.

Learn more about E. coli strains used for protein overexpression.

Feb. 10, 2015, 3:06 p.m.

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Jan. 13, 2015, 3:47 p.m.

Use Addgene's "Find Plasmids" badge on PubMed to quickly find papers with plasmids deposited at Addgene.

May 12, 2017, 12:58 p.m.

Use CcdB to enhance the efficiency of your next plasmid cloning experiment.

Dec. 8, 2016, 3:30 p.m.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

April 28, 2016, 2:30 p.m.

Addgene plasmid pages now show whether a plasmid has been cited in other articles. Use the "References" link to view the articles citing your plasmid.

June 16, 2015, 2:15 p.m.

Learn how Addgene uses SnapGene to aid in plasmid quality control and check out some tools you might find useful for your own plasmid verification processes.

Feb. 1, 2018, 3:07 p.m.

Protein tags can be used for purification, detection, localization, and more. Learn more about the molecular tool box of tags and fusion proteins.

Dec. 11, 2014, 4:26 p.m.

Learn about B. subtilis libraries, iPSC reprogramming tools, TZAP, and a plant CRISPR toolkit.

Dec. 11, 2017, 2:02 p.m.

Learn about optogenetics tools, methods for increasing CRISPR editing efficiency, CRISPR base editors, and DIY DNA ladders.

Oct. 25, 2017, 1 p.m.

Long-read sequencing has allowed researchers to identify plasmid dimers and multimers. Learn about them - and how to troubleshoot - in this blog post.

Jan. 10, 2023, 2:15 p.m.

Learn about how Addgene has shipped over 500,000 plasmids and where those plasmids go.

March 26, 2015, 3:24 p.m.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Jan. 8, 2019, 1:54 p.m.

The origin of replication is the DNA sequence which allows initiation of replication within a plasmid by recruiting transcriptional machinery proteins, enabling a plasmid to reproduce itself.

Nov. 11, 2020, 2:23 p.m.

Learn about new plasmids and viral preps available at Addgene including a yeast-based tool for protein evolution, fluorescent tools, nanobody purification, new CRISPR tools, and calcium and norepinephrine biosensors.

Aug. 18, 2020, 1:15 p.m.