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We narrowed to 98 results for: plasmid transfection

Showing: 61 - 80 of 98 results

  1. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...to sort the cells after the plasmids have been introduced by transfection or electroporation. All you ...effector nuclease (TALEN) kit consisting of 834 plasmids. The researchers showed they could use their TALEN...particular DNA sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that allow you...the genome in rapid fashion. The two remaining plasmids in the kit have the rest of the protein in place...protein that binds those 15 base pairs is take four plasmids out of the library in the appropriate combination... ligation with the four inserts and the master plasmid in one step. The kit also has other unique aspects...you are designing a TALEN on paper to a finished plasmid. With our protocol, it is feasible to produce genetically...

  2. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...more cells = more plasmid. Plasmids can vary in copy number, the number of plasmid copies supported within...cloning, transfection, or long-term storage. But what about those pesky situations where your plasmid yield...sub-optimal plasmid yields from your prep, try our tips below! How to optimize your plasmid prep We'll ...genotype, as these mutations improve plasmid stability and yield. If a plasmid is prone to recombination Use ... a strain optimized for plasmids with repeat elements, such as viral plasmids with inverted terminal repeats...co-purify with your plasmid DNA and lead to degradation of your prep. Many plasmid prep kits recommend...levels of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein expression...

  3. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...and an envelope expressing plasmid, such as our second most requested plasmid of all time, pMD2.G. Find...wide range of applications. This plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB... top requested plasmid from 2016. You may notice the “V2.0” at the end of this plasmid which indicates...prior version of this plasmid. Indeed the first version is the 15th most popular plasmid of all time, but ...envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini....1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC cloning...15 years of plasmid sharing has certainly taken us on many adventures...From moving office locations ...

  4. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... recent plasmid deposit please sign up here.          Listen to this episode of Hot Plasmids! Optogenetic...Grefen and Blatt, 2012). The 2in1 Plasmid Toolkit consists of 4 plasmid toolkits generated with 4 different...deposited the resulting optogenetic plasmid tools which includes plasmids encoding a fusion between cPYP and...targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et al., Nature Methods 2018. PubMed...as mice, C.elegans, and Zebrafish. Find Archon1 plasmids at Addgene! Piatkevich KD et al, Nat Chem Biol...

  5. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...toolbox. Natural plasmids, also called environmental plasmids, are all around us! A plasmid is a small piece...colicin-producing plasmids have been referred to as “Col plasmids” or “Col factors.” Virulence plasmids: microbial...Old Solution but a New Problem Plasmids 101: What is a Plasmid? Plasmids 101: Transformation, Transduction...F. (2023). Plasmid classifications. Plasmid, 126, 102684. https://doi.org/10.1016/j.plasmid.2023.102684...., 2001) Beneficial traits of natural plasmids Natural plasmids often convey a variety of functions that...functions of natural plasmids. Created with BioRender.com. Resistance (R) plasmids: microbial defense...example of a plasmid benefiting its host. For example, cells with a penicillin resistance plasmid can grow...

  6. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... recent plasmid deposit please sign up here.      Listen to this episode of the Hot Plasmids podcast ... (ARG) plasmids to better monitor gene expression in vivo using ultrasound. These ARG plasmids contain...responses in C. elegans. Find the split BoTN/B plasmids at Addgene. Liu Q et al., Neuron. 2019. PubMed...potentially problematic for imaging. Find the ARG plasmids at Addgene. For more details on acoustic reporters...s collection consists of a library of reporter plasmids, each containing two fluorescent proteins and ...

  7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...1: The base editor is split into two plasmids and co-transfected into cells for reconstitution. Image ...need. If you'd ever like to write about a recent plasmid deposit please sign up here.        AAV delivery...overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion...channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed...fragments. This system has been used to construct plasmids for use in model organisms such as E. coli and...

  8. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...validated editing reagents can be found on our plasmid pages. We encourage any labs performing genome ... that are an order of magnitude greater than transfection approaches. Moreover, the cost of reagents is...CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our List of gRNA Design...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ... you to find and use the plasmids you need. You can find all the hot plasmids from 2016 below. With over...over 45,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...gRNA expressing cells were then transfected with a Cas9-expressing plasmid to generate a population of mutant...

  10. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...problem of sharing plasmids, providing researchers with a one-stop shop for plasmids and vectors, and making...development of a vast number expression and reporter plasmids and the free exchange of these vectors between...laboratories. The development of the transient transfection platform for assaying transcriptional activity...increased the burden associated with identifying plasmids in the research literature and acquiring them ... feature direct links to listings of available plasmids for our curated NRs and coregulators. We look ...2005, tr22. Keep on reading! Check out Addgene's Plasmids 101 blog series. ...

  11. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...transfer plasmid), the envelope (Env) gene on a separate plasmid (envelope plasmid), and the polymerase ... yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection of all three ...different plasmids. For a retroviral system this looks like the genetic cargo in one plasmid (the transfer...three plasmids into a producer cell line, such as HEK-293T, production of viral particles, and transduction... and Pol genes are not encoded in the transfer plasmid, the component that physically integrates into ... retroviral packaging components onto distinct plasmids for safety.    Viral cargo-specific safety...services Addgene Biosafety Guide Addgene Viral Plasmids Which Type of Virus Should I Use? Resources...

  12. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent protein (fusion...dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct for this variation,...Goedhart et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2 with mVenus as a ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  13. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...Split-BioID plasmids Find CRISPR plasmids for labeling genomic regions Find CRISPR plasmids for purifying... include both scrambled RNA motifs and cells transfected with BirA* protein alone (i.e. no RNA bait). ...question that RaPID could help answer? The RaPID plasmids are available from Addgene! References Ramanathan...PubMed Central PMCID: PMC5886736. Find the RaPID Plasmids Here! McHugh, C.A., Russell, P., & Guttman, M...

  14. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ... [6], plasmid electroporation (episomal) [7, 8] and mRNA transfection [9]. Many of the plasmids used for...Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. Transdifferentiation...Weintraub, and A.B. Lassar, Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell,...Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab ...

  15. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but did...electroporation or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem... al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al. (2023). Efficient engineering...inspired and informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R., et al. (2023). StayGold...under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R., Flytzanis, N.C., Goeden...(2023) under CC-BY license.   Find AAV1-X1 plasmid here! Chen, X., et al. (2023). Functional gene ...

  16. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...as a universal donor plasmid. The CRISPaint toolkit includes several donor plasmids for protein tagging...The Jorgensen Lab recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR...repair template. SapTrap includes a prebuilt donor plasmid library containing several types of fluorescent...tag and homology arms). Digestion of the donor plasmids with SapI allows the tag, selectable marker, and...to be released (Fig. 1, Step 2-3). Since donor plasmids of the same type will produce the same unique ...different combinations of connector and tag donor plasmids from the library can be used to generate functionally... of the targeting vector and a Cas9 expression plasmid will insert the desired genetic tag and marker ...

  17. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...structures within cells. HeLa cells were transfected with plasmids encoding FPs tagged with the plasma membrane...They generated plasmids tagged with EB3 to mark the growing tips of microtubuli and plasmids tagged with ...and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head... Find the plasmids at Addgene The MoClo CRISPR/Cas Toolkit for plants includes 95 plasmids consisting...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Animal Models Q9: Is there any difference in transfection efficiency when delivering these viruses via...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors...

  19. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...multitude of techniques including co-transfection of two or more plasmids, the use of multiple or bidirectional...with a plasmid that already has the multicistronic element and reporter cloned in. In these plasmids you ...interest. Some of the plasmids listed in the table above (and their related plasmids) are designed to have... a one-stop reference guide for plasmids. We've combined our Plasmids 101 blog posts and additional resources...desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid lovers and ... an introduction to plasmids, allowing you to spend less time researching plasmid basic features and spend...May 11.) PubMed.   Read more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular biology...

  20. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...adenoviral stock (rAdV-S). HEK293 cells are transfected with the AdV plasmid construct from step #1 and allowed...down into 5 key steps: Construction of the rAdV plasmid (~1 week): The AdEasyTM system is the most popular...adenoviral vector constructs. It consists of 2 plasmids: a shuttle vector (in which the transgene of interest...how to generate the final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide...2.*Pro-Tip*: once the correct recombinant pAdV plasmid is identified, re-transform into a bacterial strain...recombination for amplification. The final purified plasmid stock should be analyzed by endonuclease restriction...transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page  ...
Showing: 61 - 80 of 98 results