...It was by serendipity that I got into the field of gene therapy, more specifically AAV-based retinal ...retinal gene therapy. The year was 2001 and I started a job as a technician in a lab using adeno-associated ...degenerative disease called Retinitis Pigmentosa. I quickly became fascinated by this emerging technology...potential for the treatment of some genetic diseases. I didn’t know it at the time, but gene therapy had fallen...s congenital amaurosis. This is the first of what I, scientists, doctors and patients hope are many more...29868583. PubMed Central PMCID: PMC5960719. 8. Trapani I and Auricchio A., 2018. Seeing the Light after 25 ...being benched due to the high cost of clinical development. As a result, Luk Vandenberghe and colleagues...

...academic postdoc. Why did I switch? Well, my long-term goal was to enter industry since I was drawn to the idea...grant-writing. The more I talked to people with industry experience, the more I heard that getting into...Desrochers, a postdoctoral scientist at AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry...major life decision, the transition felt scary. While I knew two people from graduate school who were at AstraZeneca...assure me that they were having a positive experience, I was still incredibly nervous about leaving academia...will also have to attend many more meetings. How do I get an industry postdoc? Many companies have industry...AstraZeneca. She has a background in cell and developmental biology and is applying this towards researching...

...Arabidopsis mutant that can’t set seeds. I study the development of the mutant plants, but the seeds needed...tissue for imaging or collection could contaminate it. I have used it for selecting transformation events and...Zhang, T., Sun, H. et al. Hortic Res. (2020).   I can do this by expressing a gene that rescues the ...RUBY and the construct that rescues the mutation. I will be able to easily choose the green seedlings ...these can be challenging and requires specialized equipment. Reporters for studying gene expression have similar...Willoughby is a graduate student studying plant development and cell-cell communication in the Nimchuk Lab...

...of the developer and I get to see an approach that is likely different from what I would have done. And... for open science. I hope you enjoyed learning about Addgene’s software development team and how we work...developer. This is another point of collaboration for us. I enjoy doing code reviews because it’s like a little...take turns working on different parts of the code. I think the best way to learn about how any code works...software development happening at Addgene under the hood. A significant portion of our development may not...productivity. Get to know our software development team and the development process at Addgene. Diversity supports...When you think of Addgene, chances are software development is not one of the things that comes to mind. ...

...’s. I was facing the prospect of having to do a third postdoc in order to rebuild my resume, so I had ...laboratory lease, etc. I still flip to that page in my lab notebook from time to time. I wrote in large letters...often along the lines of “I hate GMOs, but what you are doing makes sense. I can support that.” That was...in a cutting-edge synthetic biology lab Keira and I worked in the same lab for four years. It was a cutting-edge...cutting-edge synthetic biology lab focused on product development: building a plant that could sense and respond...

... et al. Although I was able to knock-in a 2xFLAG epitope into my gene of interest, I encountered low efficiency...the model organisms which I use to study gene regulation during developmental processes, CRISPR/Cas9 allows...CRISPR editing in C. elegans improves efficiency  I was fortunate – or unfortunate enough, depending on... alternate approaches. Being a “lazy” geneticist, I developed a co-selection approach relying on repair...courtesy of Jordan Ward, UCSF. In 2015 (Ward, 2015), I demonstrated that selection for repair of a temperature-sensitive...Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K (2014) Efficient chromosomal...selective marker to remain in the edited animal. Development of CRISPR/Cas9 editing strategies in C. elegans...

...but it works for me. I can often remember which paper I need to open, but when I can’t it’s an easy search...organization system that works for you. I have a folder where I dump all of my papers and they all have... it leads you to the same conclusion. Personally, I like papers that can end with some sort of model (...Scientific Manuscript by Maaike Pols, PhD, a Developmental Editor at F1000Research  ...

...management. I needed it when balancing a handful of demanding courses, a capstone paper I really wanted...creativity; I have developed these skills through school, jobs, and volunteer work, and I guarantee you...time work, bills, (at times) a social life and rest. I need it just as much in my current role as Customer... and Operations Manager at Addgene. In this role, I balance my daily tasks, meet cross-team project commitments...graduate school. These skills will be useful anywhere I work in the future; they are transferrable across ...interested in nonprofit management and international development.       Additional Resources on the Addgene Blog...

... the scientists were waiting. I look forward to sharing new developments on this project and on all the...world. Now that the service is successfully launched, I would like to thank some of the people and organizations...have such an impact on scientific materials sharing. I would also like to recognize a few scientists who ...practices and encouraging us to explore new directions. I would like to thank them for their time, dedication...Addgene Blog and our expanding website resources. I hope this gave you the impression that it took a lot...

... right way?  First of all, I want to put to rest a common misconception. I find personal bias often unintentionally...how they prefer to consume information. Personally, I would much rather read the instructions than watch...the box. There are so many ways to communicate and I wholeheartedly encourage experimentation. You never...What resources do you have?  No matter the project, I can guarantee you that either resource or time restrictions...re happy with. Know if you’ll need specialized equipment and/or expertise from the start, or if you can... media formats. Cognitively demanding format. Equipment, expense and expertise needed can increase exponentially...

... funding. I’ve been aware of these problems since I began my own career over 20 years ago, and I’m sure...misrepresent science, or misuse science, and that is what I would call bad science.”  The three chapters in this...describes the detection of gravitational waves--a story I was much less familiar with as a biologist--as an ... to be foremost in readers’ minds. Six months ago I would have nodded my head knowingly and appreciatively...slightly dated by next week.   A stepping stone When I discussed The State of Science with the five Addgene...challenges you face in your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne Kamens...identification of Green Fluorescent Protein (GFP) and its development into an essential tool in biology also falls ...

...animals than i-GONAD and is a more technically difficult process. Overall, the main drawback of i-GONAD is...Diagram of ovary and oviduct anatomy with the equipment used for i-GONAD. A micropipette is used to inject ...responsible for Easi-CRISPR have also recently developed i-GONAD as a simple method for delivery of genome editing...germline. In contrast to standard microinjection, i-GONAD is performed directly on pregnant female mice...Ohtsuka et al. compared traditional microinjection and i-GONAD knock-in efficiency using a small ssDNA, and... with a long ssDNA donor was less efficient using i-GONAD (15%) vs. microinjection delivery (25-67%). ....2017.153 PubMed PMID: 29266098 Ohtsuka, Masato, et al. i-GONAD: a robust method for in situ germline genome...

...were very anxious at this point. Here's the message I shared with the Addgenies on March 11, 2020 after ...figure out how to deliver when labs finally opened.  I was awed by the teamwork and initiative it took to...and the subsequent fluctuations in distribution. I don’t have space in this blog to call out every Addgene...new projects on track to succeed at the same time. I began approaching science funding organizations to...t raise money except occasionally for capital equipment or resources needed to launch a new service or...

... week, I felt overwhelmed. Jumbled thoughts such as “I have no idea how to write a grant!”, “I don’t have... mind. Moving forward from that first experience, I learned the details of grant writing, volunteered ...Research strategy The Research Strategy highlights i) the significance and innovation of the project and...chance to delegate funds to supplies, personnel, equipment, travel, etc. As a first time budget writer, you...

...additional mentors. I found this beneficial for my projects, my training, and when I went on the job market...: What is the most exciting story I can tell with my data?  Can I make better slides that more effectively...signing up for and how can you do it well? Below, I briefly describe some tips for a successful postdoc...unpublished information and reagents. Part of the reason I got my first faculty position was because the search...asked someone at my institute about me, even though I had not asked this person to write a letter of reference... improves your perspective like intense exercise. I highly recommend running or biking or something that...this person cares about your science and your development. Taken together, all of this means that you need...

...writing habit into beautiful graphs! I like to look at these graphs when I need that extra push to stick with...suggestions on how to make your writing less complex. While I don’t think you should accept all of the suggestions...School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student podcast...use in black. This paper talks more about the development of the De-jargonizer.   Academic Phrasebank  ...

...creative thinking. I won’t go through all nine here, but I’ll discuss a few that I frequently observe ...optimize virus production with roller skates—though I could get around the lab faster that way— but the ... and prevent groupthink? Here are some ideas that I have found helpful in my team brainstorming sessions...may feel they can share opposing opinions as well. I am sure we have all experienced a meeting where one...protocols and even our Lab Inventory Management system. I hope these tips help you to see that the creative ...form at any time and a member of our Business Development team would review it. This survey generated 26...

...., Woodard, K., Cepko, C., Guerin, K. I., Rego, M. A., Ersing, I., Bachle, S. M., Kamens, J., & Fan, M...recently released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector... Viral vectors are, after all, a delivery method. I would be remiss to talk about them without looking...from eBooks to guides to protocols, which was why I was so excited to delve into this data as we updated...Campus on the GENIE website.   Bibliography  Ersing, I., Rego, M., Wang, C., Zhang, Y., DeMaio, K. H., Tillgren...process have shaped and supported our growth and development. We’ve shared what we’ve learned, through manuscripts...

... can help you. You Can Talk to Your Editors I wish I had been more cognizant of this during my PhD....the Future of Research (FOR) Boston Symposium 2015. I personally participated as a member of the Career ...ll point out some of the highlights and takeaways I got from the symposium. The goal of the Future of...achieve success in their careers. The take-away message I took from the career panel was that there are many... making it more easily digestible/available. What I viewed as the most important take-away from this session... to trainees navigating the waters of career development. Publishing Panel The Early Career Researchers...

...the classroom. I had the opportunity to talk with Provost recently about his development of what is now...planarian regeneration into one of my own courses, I was excited to hear about how he developed his program... like an exciting opportunity for your classroom, I highly recommend you try implementing one. It's likely...learning in a year-long biochemistry laboratory: Part I – guided inquiry – purification and characterization...progenitor cell maintenance in planarians in Developmental Biology (Kimball et al., 2020). Benefits and... pathway to diversify science. Evolution and Development. 24(5):127-130. Kimball, C., Powers, K., Dustin... progenitor cell maintenance in planarians. Developmental Biology. 457:119-127. Knutson, K., Smith, J....