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We narrowed to 71 results for: prime editing

Showing: 61 - 71 of 71 results

  1. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... area for future AAV application to epigenetic editing. This area is discussed in our recent review.  ... Addgene Blog Using virus in your research - a primer for beginners Adenoviral delivery of CRISPR/Cas9...

  2. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...of interest is a key step for CRISPR/Cas9 gene editing. Dr. Masahito Ikawa has created a GFP reporter ...that was used by Chen and colleagues for genome editing inXenopus tropicalis. (Guo et al., Development....Development. 2014..) A new, higher specificity genome editing system that combines TALENs and CRISPRS. Developed...select the appropriate pair of vector and gene primers from Table 2 of the associated publication which...of sequence homology between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and...

  3. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to Verify Your...Electrophoresis Bacterial Transformation How to Design a Primer Creating Bacterial Glycerol Stocks Inoculating ... Videos What is a Plasmid? How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating...Analysis We also introduced guides — practical primers to help users get started with tools like CRISPR...

  4. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...Learn How Piggy Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from Grad Student...amplified by a particular primer pair. Then, by leveraging qPCR data from multiple primer pairs, one can infer...build new registers. The primer binding sites can also be used to design primers to read states by qPCR ... each state of a register enables the design of primer pairs that PCR amplify in some states but not others... regular PCR may not be very informative – each primer pair will amplify even if its target states are... promoters, and genes) were built with flanking primer binding sites (designed to have 20-30 nt length...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...resulting in a rapamycin-inducible Cas9 for genome editing. Without rapamycin treatment, the Cas9(N)-FRB-NES... S. thermophilus which have been used for gene editing in human cells without cross-talk in cognate sgRNA...show that, with the many nuclease based genome editing techniques out there (and particularly CRISPR),...expressed is amplified using a gene-specific forward primer containing the desired restriction site (the MCS...has several options) and a pUCX-specific reverse primer containing the missing codons from the antibiotic...if a small contaminating PCR product formed from primer dimers and containing the missing codons from the...resistance gene is inserted. It is important that primers be designed to minimize the possibility of dimer...

  6. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources on Addgene.org Checkout our... of the improvements: SHERLOCKv2 uses far less primer in the pre-amplification step allowing for greater...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...bacterial streaks, if you prefer). If you wish to edit your plasmid data after submission, please email...into the plasmid backbone, and what sequencing primers can be used to verify it. If your plasmid has multiple...

  9. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...Adapted from Wong, 2018   Primers Design of primers and probes Primer design, of course, could easily...probes/primers often offer higher allelic specificity, due to the need for the target:probe/primer bonds... included in the 5’ primer Yes Yes. Agarose gel  LUX fluorogenic primers Low Single self-quenching... molecule connected to a primer  No Yes Agarose gel Sunrise primers   Low Reporter and quencher... weighted towards the ends of the primers. Concentration of primers should be between 100 and 400 nm and...temperature 5-10 degrees above the primer and be at least 25 bp away from each primer, ideally located close to...either the forward or the reverse primer.  There is a list of useful primer design resources in the References...

  10. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...extracted and subject to isothermal amplification by primers that target the E and the N2 gene of SARS-CoV-2...reverse-transcribed target sequence that are present close to the primer recognition sites and are added separately. Using...fluorescence upon cleavage. Upon amplification by the RPA primers, the Cas12a-crRNA binds to the complementary region...equipment, Fluorescent emission reader Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique...sequence is amplified by PCR/RPA using biotinylated primers. These are then immobilized with streptavidin coated...non-PAM regions, PAM can be engineered into the primers. This method does not depend on collateral cleavage...

  11. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...stuff: the experiments! In our final post in this primer on optogenetics we’ll delve into some of the important... neurons. Current protocols in neuroscience / editorial board, Jacqueline N Crawley  [et al]. 2012;Chapter...
Showing: 61 - 71 of 71 results