We narrowed to 78 results for: sas;

1. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...protein, we have used Western blots, dot blots, ELISAs, and enzyme assays to select both over- and under-expressing...

2. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...

3. Hot Plasmids - August 2020

   Type

   Blog Post

   Published

   Aug. 18, 2020, 1:15 p.m.

   ...into genomic DNA with the help of the piggyBa transposase. Image from Hazelbaker et al., 2020. The lab...

4. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...editing with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat. Biotechnol. 2020 387...

5. Neuronal labeling with Spaghetti Monster

   Type

   Blog Post

   Published

   Aug. 14, 2018, 12:42 p.m.

   ...design in public schools and was started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon...

6. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...27446908. PubMed Central PMCID: PMC4923196. 8. Sassolas, Audrey, et al. “Optical detection systems using...

7. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   Published

   Aug. 18, 2016, 2:30 p.m.

   ...Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae...

8. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   ...system, as is commonly used in western blotting or ELISAs.  A popular method for indirect labeling employs...

9. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

   Type

   Blog Post

   Published

   May 5, 2020, 1:15 p.m.

   ...cellular aspects of CRISPR/Cas immune systems in the Sashital Lab, Iowa State University. She is actively interested...

10. Five Popular Model Organisms, Part 2

    Type

    Blog Post

    Published

    Sept. 29, 2020, 1:15 p.m.

    ...including plasmids for use in CRISPR and Tol2 transposase induced transgenesis.  Xenopus Xenopus, or frogs...

11. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ... institute is located. For example, salaries in Kansas versus salaries in NYC will vary widely. Therefore...

12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ...Last Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility...

13. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ...editing with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 38:824–844...

14. Antibodies 101: Antibody Engineering and Directed Evolution

    Type

    Blog Post

    Published

    Aug. 5, 2025, 1:15 p.m.

    ...support common research tools like western blots, ELISAs, immunoprecipitation, and flow cytometry. The better...

15. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ...research setting, but these events could have disastrous health effects in the clinic. NGS-based methods...

16. With an Eye Towards the Future, We Look Back at the March for Science

    Type

    Blog Post

    Published

    Oct. 24, 2017, 1:54 p.m.

    ...funding are critical to averting a potential climate disaster. However, policy makers use these two fingers ...

17. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...5): p. 1174-1174 e1. PubMed PMID: 22632979. 14. Sasaki, K., et al., Robust In Vitro Induction of Human...

18. 27 Hot Plasmids from 2016

    Type

    Blog Post

    Published

    Dec. 22, 2016, 3:03 p.m.

    ...VPR expression plasmid that uses the piggybac transposase to integrate dCas9-VPR into the genome of target...