...the names of everyone at Addgene, and send random sets of 4 people out to lunch.   I think these random...food, and these lunches would provide a friendly setting for people in different labs to chat informally...

...longer-lasting effects. Building your chemogenetic system To set up a chemogenetic system, you’ll need to choose a...retain their intracellular interactions and still set off the same pathways, giving rise to the same cellular...cellular responses. The cellular response set off by a DREADD depends on what G-protein and, consequently...drugs that must diffuse throughout the body, the onset of receptor activation is delayed and takes on the...

...Controlling stimulation Invasiveness  Choosing the best set of tools therefore depends on what you are looking...with all tools, both approaches suffer from unique sets of off-target side-effects that should be carefully...the diffusion of drugs throughout the body, the onset of receptor activation takes minutes. As a result...can be injected, this is not as precise and the offset of stimulation is gradual. Invasiveness Both optogenetic...DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad...

... fun every day.  With these three main values we set out to radically redesign our homepage to reflect...reinforcing the three values with each element. We also set out to make sure the page looked clean, professional...

...forward? Most projects typically require a diverse set of skills. It is important to know who in the lab...benchmarks. Think about what your personal goals are and set milestones along the way so that you don't get stuck...

...in yeast imaging, categorized by specific filter sets and experimental output requirements. Select from...GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. cerevisiae, with the additional...

...spectrophotometers have an IgG protein setting. If yours does not, be sure to set the absorbance to 280 nm (Pace... antibody is not IgG, make sure to look up the setting for your isotype.  Antibody concentration may also...

...high-fidelity” junction sets for both the 12- and 24-fragment versions of the lacI/lacZ cassette were designed ...- or 24-fragment test systems was performed by setting up assembly reactions in which a single component...sequences under standard reaction conditions. This data set allows quantitation of sequence-dependent ligation... the ability to correctly assemble a lacI/lacZ cassette (designed by NEB for use in optimization of assembly...sequence for beta-galactosidase in the lacI/lacZ cassette. Additional confirmation of accurate assemblies...12- and 24-fragment assemblies of the lacI/lacZ cassette, and illustrates how the volume of the 1 ml outgrowth...and assembly efficiency Five fragment lacI/lacZ cassette assembly was easily achievable with high levels...

...performance via the depletion of a gold standard set of genes (1,580 essential and 927 non-essential genes...off-target effects. The Dolcetto library contains two sets of sgRNAs; A, the top 3 ranked sgRNAs, and B, the...sgRNAs were selected per gene and divided into two sets, A and B. Sanson et al. demonstrated that the Calabrese...cutting. A Dolcetto library containing only 3 sgRNAs (setA) also outperformed CRISPRi libraries with 10 sgRNAs...

... denotes intersection and the – operator denotes set difference. That’s the intuition for how genome math...1: Genome math illustrated. Intersect (left) and set minus (right). Enhancers are regions of DNA that...Intergenic CRISPRs Yours truly generated the DHS data set by starting with all of the DHS sites from 125 different...

... recombinant antibodies. The first collection, a set of integrin antibodies, will be available on Addgene...

...polymorphisms Codon optimization tool: To access this tool, set up an IDT account. Figure 2: Addgene's Analyze...Assembly Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions Cloning... of validated antibodies for mouse, human, and marmoset brain IHC.  FPbase: A data repository for fluorescent...

...applications. Recently, the Spiess lab developed a set of functionalized nanobodies to study retrograde ... from the cell surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...Sontheimer and his group from the University of Massachusetts Medical School have developed a group of plasmids...

...The Arabidopsis Biological Resource Center (ABRC) set out to remedy that with the establishment of an outreach...reaching out to us at [email protected] for help setting up an ordering account. Using Arabidopsis in the...

... the team and bring on scientists with a diverse set of experiences. Let’s meet the newest board members...through direct industry collaborations, grants, or setting up commercial and nonprofit companies. His lab ...

...tested GeoCas9’s love of heat in two ways. The first set of experiments tested the ability of GeoCas9 RNPs...maintain activity following heat treatment. The second set of experiments tested the ability of GeoCas9 RNPs...

...ensure accurate data analysis, include a complete set of controls. Ideally, every flow cytometry experiment...flow cytometry experiment, users must also prepare sets of controls to aid with compensation. Compensation.... The fluorescence-minus one panel will help you set up gating during data analysis.  We hope that this...analysis and some useful background information for setting up antibody based flow cytometry experiments.   ...cell? Or perhaps, you need to analyze a specific subset of cells in a complex population. Have you spent...this technology can be used to isolate a specific subset of cells in a population in a procedure termed ...instruments have specialized components that can divert a subset of cells. During FACS, the sample stream oscillates...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers... experts that complement your (scientific) skill set and is therefore very useful for complex projects... point of talking to them at a seminars, or even set up regular meetings. Meeting people outside your ...team or lab, you’re likely to be seen as a huge asset! As is true for any skill, there is a learning curve... from others and meeting people with different mindsets and approaches can truly broaden your mind. When...

... to secure your first (R-level) research grant.  Set your timeline from there, then start identifying ... use these tools Outline the knowledge and skill sets needed to do your science. This will help you identify...entering a win-lose situation that can ultimately set you up for failure. Don’t be afraid to walk away ...with others will help prove that you will be an asset. 4. Always ask, “Is there an alternative?” As scientists...

...follow our timelines! Prepare a research plan to set yourself up for success prior to your departure for...reagents are onsite before you need them, and you are set up for success. If you are communicating with a professor...