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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 74 results for: sgrna

Showing: 61 - 74 of 74 results

  4. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ... (Yu et al., Nat Chem Biol). Mutate the PAM or sgRNA sequences Cas9 likely goes through multiple rounds...

  5. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... as WU-CRISPR (Wong et al., 2015)) or our lab’s sgRNA scorer 1.0 (Now 2.0) (Chari et al., 2015) and picking...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...The library was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses...that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides against...obstacle. With CRISPR-X, dCas9 is used to recruit an sgRNA with MS2 hairpin binding sites, which then recruit...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...

  12. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.” Genetics 200(2) (2015): 431-441. PubMed PMID:...Assembly. Using BsaI, gRNAs are cloned into one of 12 pYLsGRNA plasmids, which contain various promoters and ...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...
Showing: 61 - 74 of 74 results