Custom CRISPR Screens & the Green Listed Software
Type
Blog Post
Published
July 11, 2017, 2:30 p.m.
...It can be very time consuming to design 5 guide RNAs (gRNAs) targeting each of the 1000 genes you’d like...phenotypes that can be efficiently measured at the single cell level), are separated by, for example, cell...28414855.
2. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target... gene is not targeted by only one gRNA, instead many different gRNAs are used for each gene. At the moment...contain 4-12 gRNAs/gene, which results in libraries containing up to around 200,000 different gRNAs. It has...should use ~400X more cells than gRNAs in a screen, to ensure that each gRNA has a good chance to contribute...multiple gRNAs targeting all genes in the indicated species and were suggested to be the best gRNAs by the...