Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 93 results for: tac promoter

Showing: 61 - 80 of 93 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...studying how that one protein interacts with that one promoter can tell you, it can be easy to get lost in the...factors). As the Yamanaka lab's primary Addgene contact for many years, I've been looking forward to seeing...

  3. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...science and engineering. “Fix the Institutions” promotes gender equality in careers through structural ... gender analysis for innovation and discovery. Tackling sex and gender in research Governments, universities...

  4. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA library... elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. They used CRISPR...Find the lentiviral preps at Addgene hSyn1-SIO-stGtACR1-FusionRed is a Cre-dependent, soma-targeting inhibitory...

  5. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...a requirement. Your manager: Unlike academia, promotions in industry are based on people’s ability to ...which means you’re more likely to have the right contacts and get your resume pulled to the top of the pile...position. Work your LinkedIn connections and other contacts to get that referral and find out more about the... postdoctoral experience Read Joanne Kamen's 8 tactics for choosing a lab Resources on Addgene.org Find...

  7. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow...the morale of postdocs, and indeed we have been contacted by a number of postdocs who are now planning unionization...

  8. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression pattern...pattern and an auxin inducible degron (AID) sequence attached to your protein of interest. TIR1 interacts with...

  10. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...skills. This is an era of social media and self-promotion, and personality is playing an increasingly important...on fluorescent proteins and their applications, tackles much broader issues in this brief volume. He examines... Watsons.” In Part Four, “New Science,” Zimmer tackles more recent examples of scientific advances, and...

  11. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...transgenic genome will simply express from whatever promoters were included in the vector (or are nearby in ...after all, among the more virulent viruses (it attacks the immune system directly). It may seem odder ...6 x 106 virus-like particles that we come into contact with every day (Prussin et al., 2015), only a small...what these vector particles do once they come in contact with target cells (Fig. 3).     Figure ...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...walk into any supermarket now without seeing a promotion for antibiotic-free, farmed meats. We can focus...antibiotics, like penicillin. These specific drugs attack the cell wall of bacterial cells; however, Mycoplasma..., is fight smarter, not harder. We can utilize tactics like rotating antibiotic treatments, so that exposure... public health.     References 1. O'Neill, J. "Tackling drug-resistant infections globally: final report...

  13. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...incorporating it into a fourth plasmid. In addition, the promoter of the 5’ long terminal repeat (LTR) is mutated...supernatant is collected, filtered, and titered. A major obstacle in obtaining usable lentivirus is producing a ...

  14. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...they ordered contains the correct inserts, tags, promoters, and other critical functional components. Fun...If the QC team identifies a QC issue, they will contact the depositing lab to review the discrepancies....

  15. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, ...reducing the time it takes to image through a large z-stack (8).  One limitation of LSFMs using single side ...illumination, especially with large tissue, is that any obstacles (e.g. air bubbles or high concentration of fluorophores...

  16. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...feasible. Through the virtual systems created and promoted during COVID-19, online meetings with current ...other. Before COVID-19, financial and logistical obstacles could make touring potential programs and universities...

  17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...continuous outflow of phage, increasing stringency and promoting survival of only the broadest-targeting variants...available near the locus you’d like to modify. To tackle the PAM problem, researchers have engineered alternative...

  18. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...complexed with a gRNA that directs them to the promoter of a gene of interest, they have been shown to...target site, Cas9’s DNA binding capability remains intact while its nucleolytic activity is eliminated (Fig...

  19. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ... difference between them lies in the choice of promoter for ωRNA (reRNA) expression (see Figure 1). Both...5: Colony Screening Use Primer 92F (5′-cattacgcaattggacgacaac-3′) and Oligo 2 (specific to your target.... If you have specific questions, feel free to contact the Molla Lab (X account: @Kutub_joy). Dr. Kutubuddin...

  20. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...are binary gateway vectors and can be used for promoter swapping and generating N and C terminally tagged...transfer DNA (tDNA) into the genomes of the plants it attacks Growing the transformed agrobacterium culture Dipping...
Showing: 61 - 80 of 93 results