... sensor, he will construct plasmids containing promoter regions that activate fluorescent protein transcription...and to Seeding Labs who helped us coordinate and promote the awards. Additional Resources on the Addgene... has studied various aspects extreme life on earth including extremophile microorganisms, biomarkers, ... to pyrazinamide (PZA) (a drug commonly used to treat tuberculosis) and in identifying new drug targets...biofilm formation. The quest for plant compounds to treat complications of diabetes Gloria Otito Izu, PhD...infusions of L. taraxacifolia leaves are widely used to treat several diseases. Her goal is to identify and isolate...blocked, resulting in reduced blood flow. Novel treatment options, as well as non-pharmacological solutions...

...activated, this DREADD acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor...more accurate cell and tissue targeting. While promoters and AAV serotypes can sometimes be used to target...2-N-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 N protein  pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro...pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro) are also...SARS-CoV-2 M protein pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a ...

...expression plasmids in a high copy backbone with a T7 promoter driving multiple combinations of WT, codon optimized...which drive expression using either TEF1 or GAL1 promoters. These vectors are intended for the high level...race to find vaccines, therapeutics, and other treatments to stem the spread of COVID-19. That’s why we...sites (thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include...expression and purification of SARS-CoV-2 proteins for downstream experiments and offer flexibility in tag, cleavage...requested the plasmid. By leveraging our core strengths of intensive screening and the use of high throughput...

...dCas9 along with transcriptional repressors to the promoter region of the gene of interest. dCas9-based repressors... and a dCas9-KRAB fusion, both targeted to the promoter region. Cas9 activators include Cas9 fused to ...the Suntag array of factors, all targeted to the promoter region.   Ok, I have a Cas9! What do i do ... genome.  2) The target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence...

...different promoters followed by leader sequences (encoding small leader peptides) downstream of which ... vectors within this kit contain a variety of promoters to facilitate gene expression in bacteria, yeast...different genes was predictable based solely on the promoters and Shine-Delgarno sequences used in this so-called...transcription from resulting in the production of a downstream peptide, the transcription terminators in this...BIOFAB Plasmid Set contain terminators of varying strengths centered within GFP/RFP bicistronic reporters ...

...targeted gene from its promoter, creating a loss-of-function allele as well as a promoter-FP fusion for the...for the CRISPR/Cas9 revolution!   References Streamlined Genome Engineering with a Self-Excising Drug ...

...that flank the gene cassette, which contains a promoter, the gene of interest, and a polyA signal. Created...sequences are from AAV2 because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs...limited, but this area of research is important for downstream AAV applications.  Tips & FAQs Due to their high...

...for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, was co-opted...Affordable to culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to...

...unstable fluorescent proteins under the control of promoters responsive to water or nutrient levels can help...fluorescent protein reporter under the control of promoters that control cell cycle gene expression.  Additional...serotonin reuptake inhibitors are frequently used to treat depression and anxiety, but these drugs are often...

...efficient AAV prep for a 4.2 kB insert with the CMV promoter using the AAV-BR1 capsid? A: It is our understanding...4.7kB.  If your insert alone is 4.2 kB and CMV promoter+enhancer is 0.5 kB, then you would be oversized... reliable quantitation. Differences in sample treatment across methods can affect reported titer by either...

...search scoped to viral preps shows filters for promoter and serotype, among others. We also created a ...example, there is only one viral prep filter for promoter “Syn”, that, when selected, will match variations...members from the Customer Service, Data Curation, Outreach, Product, Scientist, and Software teams. We knew...

...protein whose coding sequence is closest to the promoter will be expressed. This system differs from Brainbow...translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant YFP that .../FRT recombinase. When placed under different promoters, Brainbow and Flpbow can be used to label distinct...Drosophila. Why Brainbow? Brainbow highlights the trend in biology towards single-cell resolution imaging...

...suitability of various antibiotic resistance markers, promoters, and inducible gene expression systems in bee ...fusion is under control of the arabinose-inducible promoter. Cells are first induced with arabinose to begin...designed to bind and detect nearly any RNA sequence. Downstream of the hairpin is a ribosome binding site (RBS...responds to infection, dysbiosis, and antibiotic treatment. To facilitate these studies, Pamela Silver’s ...

... Atlas (NURSA), one of our primary goals is to promote an understanding of how these processes are regulated...upon a context defined by ligand, cell type, and promoter, organizing the expression of functional groups...transported from cells, traverse the body through the bloodstream, and eventually elicit their actions on other...drugs currently available or being developed to treat a multitude of therapeutic indices, including hypertension...

...either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al., ...known as SpyLigase and is a protein domain that promotes the formation of an isopeptide bond between 2 ...insert fragments of DNA containing basic parts (promoters, UTRs, coding sequences, terminators, etc) into... creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, ...individual types of pre-cloned insert modules (plant promoter, N-terminal tag, coding sequence of the gene of...Biotech. 2013. Dec 23.   Highly bright and extremely dim GFPs The availability of the three dimensional...unprecedentedly-high brightness, bfloGFPa1, and the other with extremely-dim brightness, bfloGFPc1. The plasmids used for...

...customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation...plasmid from the Zhang lab expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and...

...kit contains 96 parts including terminators and promoters, CRISPRi parts, selectable markers, transformation...determined protein expression levels from different promoters, and inhibited gene expression using CRISPRi. ... acceptor vectors from the MoClo Toolkit from Sylvestre Marillonnet’s lab to develop synthetic biology...

...vector containing homologous ends to the breakpoint promotes homologous recombination of the targeting vector... process. The addition of small molecules that promote homologous recombination or the use of long single-stranded...recessive traits and their impact on diseases or treatments, but are not useful for genetic engineering. ...initially inhibited by the presence of a stop cassette upstream of the target gene in the targeting vector. The...

...expresses multiple reprogramming factors driven by one promoter. While lentivirus has similar rates of reprogramming...control. Additionally, Sendai virus doesn’t rely on promoters for transcriptional regulation, so it requires... mini-plasmids. They contain only a eukaryotic promoter and the cDNA(s) to be expressed and they don’t...phosphatase, expression of embryonic proteins, and promoter demethylation and expression of endogenous pluripotency... cellular defenses against ssRNA: phosphatase treatment, substituting cytidine with 5-methylcytidine, ...

...very realistic. Envisioning GFP as a substrate promoting dimerization, Tang et al. tested pairs of their... they activate gene expression from different promoters. Moving to an in vivo system, Tang et al. electroporated...at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest blocks transcription when...