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We narrowed to 201 results for: tre promoter

Showing: 61 - 80 of 201 results

  1. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...are therefore good temporal reporters to measure promoter activity, for example. Useful positive controls...dark after the transformation. Use introns Plant promoters can be recognized or leaky in Agrobacterium. This...of the GUS gene with the commonly used CaMV 35S promoter in Agrobacterium. This can be easily avoided by...more easily quantify the effect of the different treatments on transformation success. Some reporter gene...

  2. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...to a plasmid that lacked a promoter — only CAST integration of the promoter could turn the gene on. Once...integrate a payload sequence into genomic DNA downstream from the Cas target. Conveniently, the process...to integrate large sequences into genomic DNA downstream of gRNA-specified sites. Created with BioRender.com...pressures: high efficiency helps them spread, but also stresses the host. The researchers suspected natural evolution...emerging world of gene therapy, highly tailored treatments to specific mutations require individualized ... of loss-of-function mutants could provide a streamlined, one-size-fits-most approach. evoCAST is an important... important step toward that kind of gene treatment. The researchers also compared evoCAST to the Liu lab...

  3. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ... researchers must be extreme self-starters and especially good at self-promoting—No one is going to tell...diversity and acceptance can be an issue in getting promoted (especially race and gender) Interpersonal issues...especially with peer mentors, to discuss your strengths, weaknesses, interests and passions is another...

  4. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...limited by the lack of well characterized inducible promoters. Far removed from the bio-industry’s vision of... the same dCas9 protein. By using an inducible promoter to modulate dCas9 expression, multiple scRNA transcriptional...genes or to introduce heterologous genes, but the trend of research has shifted in recent years toward tinkering...regulators (Zalatan et al. 2015).  These scaffolds are stretches of non-coding RNA interspaced with linkers that...

  5. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ... sensor, he will construct plasmids containing promoter regions that activate fluorescent protein transcription...and to Seeding Labs who helped us coordinate and promote the awards. Additional Resources on the Addgene... has studied various aspects extreme life on earth including extremophile microorganisms, biomarkers, ... to pyrazinamide (PZA) (a drug commonly used to treat tuberculosis) and in identifying new drug targets...biofilm formation. The quest for plant compounds to treat complications of diabetes Gloria Otito Izu, PhD...infusions of L. taraxacifolia leaves are widely used to treat several diseases. Her goal is to identify and isolate...blocked, resulting in reduced blood flow. Novel treatment options, as well as non-pharmacological solutions...

  6. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...activated, this DREADD acts through the Gq pathway and promotes neuronal firing. Plasmid Serotype Name Depositor...more accurate cell and tissue targeting. While promoters and AAV serotypes can sometimes be used to target...2-N-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 N protein  pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro...pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro) are also...SARS-CoV-2 M protein pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a ...

  7. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    Published
    May 19, 2020, 1:15 p.m.
    ...expression plasmids in a high copy backbone with a T7 promoter driving multiple combinations of WT, codon optimized...which drive expression using either TEF1 or GAL1 promoters. These vectors are intended for the high level...race to find vaccines, therapeutics, and other treatments to stem the spread of COVID-19. That’s why we...sites (thrombin and TEV), and C-terminal tags (StrepII, 3xFLAG, 6xHIS, MBP, Myc). These constructs include...expression and purification of SARS-CoV-2 proteins for downstream experiments and offer flexibility in tag, cleavage...requested the plasmid. By leveraging our core strengths of intensive screening and the use of high throughput...

  8. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...dCas9 along with transcriptional repressors to the promoter region of the gene of interest. dCas9-based repressors... and a dCas9-KRAB fusion, both targeted to the promoter region. Cas9 activators include Cas9 fused to ...the Suntag array of factors, all targeted to the promoter region.   Ok, I have a Cas9! What do i do ... genome.  2) The target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence...

  9. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...different promoters followed by leader sequences (encoding small leader peptides) downstream of which ... vectors within this kit contain a variety of promoters to facilitate gene expression in bacteria, yeast...different genes was predictable based solely on the promoters and Shine-Delgarno sequences used in this so-called...transcription from resulting in the production of a downstream peptide, the transcription terminators in this...BIOFAB Plasmid Set contain terminators of varying strengths centered within GFP/RFP bicistronic reporters ...

  10. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...targeted gene from its promoter, creating a loss-of-function allele as well as a promoter-FP fusion for the...for the CRISPR/Cas9 revolution!   References Streamlined Genome Engineering with a Self-Excising Drug ...

  11. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...that flank the gene cassette, which contains a promoter, the gene of interest, and a polyA signal. Created...sequences are from AAV2 because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs...limited, but this area of research is important for downstream AAV applications.  Tips & FAQs Due to their high...

  12. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...for its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, was co-opted...Affordable to culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to...

  13. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    Published
    June 4, 2019, 1:02 p.m.
    ...unstable fluorescent proteins under the control of promoters responsive to water or nutrient levels can help...fluorescent protein reporter under the control of promoters that control cell cycle gene expression.  Additional...serotonin reuptake inhibitors are frequently used to treat depression and anxiety, but these drugs are often...

  14. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...search scoped to viral preps shows filters for promoter and serotype, among others. We also created a ...example, there is only one viral prep filter for promoter “Syn”, that, when selected, will match variations...members from the Customer Service, Data Curation, Outreach, Product, Scientist, and Software teams. We knew...

  15. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...efficient AAV prep for a 4.2 kB insert with the CMV promoter using the AAV-BR1 capsid? A: It is our understanding...4.7kB.  If your insert alone is 4.2 kB and CMV promoter+enhancer is 0.5 kB, then you would be oversized... reliable quantitation. Differences in sample treatment across methods can affect reported titer by either...

  16. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...protein whose coding sequence is closest to the promoter will be expressed. This system differs from Brainbow...translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes a mutant YFP that .../FRT recombinase. When placed under different promoters, Brainbow and Flpbow can be used to label distinct...Drosophila. Why Brainbow? Brainbow highlights the trend in biology towards single-cell resolution imaging...

  17. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...suitability of various antibiotic resistance markers, promoters, and inducible gene expression systems in bee ...fusion is under control of the arabinose-inducible promoter. Cells are first induced with arabinose to begin...designed to bind and detect nearly any RNA sequence. Downstream of the hairpin is a ribosome binding site (RBS...responds to infection, dysbiosis, and antibiotic treatment. To facilitate these studies, Pamela Silver’s ...

  18. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... Atlas (NURSA), one of our primary goals is to promote an understanding of how these processes are regulated...upon a context defined by ligand, cell type, and promoter, organizing the expression of functional groups...transported from cells, traverse the body through the bloodstream, and eventually elicit their actions on other...drugs currently available or being developed to treat a multitude of therapeutic indices, including hypertension...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al., ...known as SpyLigase and is a protein domain that promotes the formation of an isopeptide bond between 2 ...insert fragments of DNA containing basic parts (promoters, UTRs, coding sequences, terminators, etc) into... creating a single transcriptional unit (Ex: a promoter, 5’UTR, coding region, and terminator). Next, ...individual types of pre-cloned insert modules (plant promoter, N-terminal tag, coding sequence of the gene of...Biotech. 2013. Dec 23.   Highly bright and extremely dim GFPs The availability of the three dimensional...unprecedentedly-high brightness, bfloGFPa1, and the other with extremely-dim brightness, bfloGFPc1. The plasmids used for...

  20. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation...plasmid from the Zhang lab expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and...
Showing: 61 - 80 of 201 results