We narrowed to 772 results for: INA
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TypeBlog PostPublishedJune 24, 2014, 3:59 p.m....Japanese ostracod(Cypridina noctiluca)Sea firefly(Cypridina hilgendorfii) 465 62 Cypridina (Vargulin) luciferin...
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Plasmids 101: Mammalian Vectors
TypeBlog PostPublishedMarch 25, 2014, 3:15 p.m....considered an entirely new cell line from that of the original parental cells. Positive selection in mammalian... HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits termination step of translation 2-10 ug/mL G418/Geneticin... Inhibits protein synthesis; premature chain termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3, CHO,... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....support the community in developing better, more sustainable, easily sharable products. Open-Source Microscopy...makerspace networks and training users via workshops, seminars, or hackathons greatly increases accessibility...brings stronger networking across laboratory and disciplinary boundaries and can allow for innovative organizations... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....since it is impossible to check all possible combinations of biology and equipment. Because the performance...(Goedhart et al, 2011) allows for a precise determination of brightness in cells, under realistic experimental...fluorescent protein in the host cell, which is a key determinant of the practical brightness. Therefore, practical... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog PostPublishedMay 9, 2017, 2:12 p.m....s ability to process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive...processing, i.e. tRNAs or Csy4 cleavage sites. This eliminates bulk from crRNA expression plasmids resulting...crRNA array (bolded red arrow). This undoes the original reversing of the direct repeats, leading to expression... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog PostPublishedFeb. 19, 2019, 1:36 p.m....auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S. in accounting... on average 20 PhD’s a year and we have a very dominant presence in the biotech sector in both patent ... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....log off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I...help implement solutions for the stakeholders. Finally, software product managers test the solutions with... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m....method (M-CREATE) by: Brian O'Neill The Viviana Gradinaru Lab has previously developed the multiplexed version...mutations. These plasmids contain catalytically inactive dCas9 and microbial SSAPs (dCas9-SSAP) which insert...specific transcription response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...are sold in packages of 10…) If you missed the webinar, you can watch the presentation and Q&A session...recent review. And that's a wrap on our AAV webinar! Special thanks to our panel of experts, Tim Miles... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m.... by geneticists to study gene function. In this final installment, I’ll provide a brief introduction as...transgenes in different loci allows for genetic recombination so that the transgenes can be inherited on the...Engineering Posts Use FLEx Vectors for Site-Specific Recombination Resources at Addgene.org Browse Drosophila...