We narrowed to 816 results for: LAS
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TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....Kotton Learn How Plasmid Sharing Speeds Science Resources at Addgene.org Order Plasmids from Addgene! ...individual and community science projects, we offer classes, workshops, book clubs, seminars, and social events...popular. People kept asking if we were teaching classes at the lab, so in December 2015 we held our first...started. For example, Timothy Stiles joined BosLab last year with a computer science degree and had zero...to engineer microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast...
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With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m....receive transfer plasmid DNA that you can use along with lentiviral packaging plasmids to create your own...rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene...helped distribute many of these technologies in plasmid form and are proud to have facilitated their fast...fast adoption. However, in many cases the plasmids themselves are only the starting point for the production... use; after receiving a pooled gRNA library in plasmid format, a researcher must amplify the library, ... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....often incorporated into the cells via plasmid. Third, a second plasmid will be needed to introduce the gene...and synthetase introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new...the cell’s genome, instead of incorporation via plasmid or media. Theoretically, if you put all these together...Blue Flame award to identify highly requested GCE plasmids. But they’d also love scientist interested in,...and resources, and then checking out all of the plasmids and bacterial strains (available at Addgene for... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog PostPublishedJuly 6, 2017, 1:59 p.m....Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids Find Kits for Cancer Research ...immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the immune system and ...analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited...deposited at Addgene was used to produce the glioblastoma model for this study (Ref 6)). In addition, it... immunology resources. If you have a great new plasmid you’d like to share with the scientific community... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....biological tissue. To prove this, simply hold a flash light to the palm of your hand. Although white light... that when it reaches the interface between the glass coverslip and a sample in aqueous buffer it is completely...due to the refractive index mismatch between the glass and the water-like buffer the sample is immersed...fluorophores within a few hundred nanometers of the glass/water interface. Super resolution microscopy Super...sensitive detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment...very gentle on the sample as the majority of the laser light is reflected away from the sample and excitation... Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m.... with the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...highlight their research and how they will use plasmids and viral preps from Addgene to further their ...BeHeard award, the ACD will use Addgene’s RESOLUTE plasmid collection to perform a functional screen in mammalian...therapeutic development,” says Iyer. Since the RESOLUTE plasmid collection consists of human and murine solute ...contraction defects. The lab also plans to use CRISPR plasmids to generate deletions of candidate genes in euploid...research questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....recognize! Distributing plasmids, viral service, and more For the first time in the last couple of years, ...to worry about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% ...distribution to ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our ...Speaking of the website, we now have a UI/UX team. Last time I checked we were ranked as the 21,806th most...Addgene Resources on the Addgene website Find plasmids for your research Learn about our viral service... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....Split-BioID plasmids Find CRISPR plasmids for labeling genomic regions Find CRISPR plasmids for purifying...literature and analyzing data from The Cancer Genome Atlas, Ramanathan et al. found evidence that suggests ...question that RaPID could help answer? The RaPID plasmids are available from Addgene! References Ramanathan...PubMed Central PMCID: PMC5886736. Find the RaPID Plasmids Here! McHugh, C.A., Russell, P., & Guttman, M... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....quality, growth, and legal status of thousands of plasmids a day, like we do at Addgene, pen and paper will..., of course, within the lab. In order to get a plasmid to your lab bench, we need to (this is a little... someone has put in a request for a particular plasmid and now it’s time for our lab technicians to retrieve...tray or a freezer. Some are disposable, like a plastic tube. Talk with your lab team about how they would...to be situations where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone...Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog PostPublishedDec. 16, 2015, 3:30 p.m....evidenced by all the CRISPR publications, press, and plasmids out there, it’s obvious that CRISPR is a ground-breaking...compared to eSpCas9(1.1) and SpCas9-HF1. The HypaCas9 plasmids are available from Addgene here! Future possibilities... (2015): aad5227. PubMed PMID: 26628643. Find plasmids from this publication at Addgene. 2. Nishimasu..." Nature (2016). PubMed PMID: 26735016. Find plasmids from this publication at Addgene. 5. Chen, JS...." Nature (2017). PubMed PMID: 28931002. Find plasmids from this publication at Addgene. Resources on... Resources on the Addgene Website Find CRISPR Plasmids Browse our CRISPR Guide Pages Find Genome Engineering...