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We narrowed to 772 results for: INA

Showing: 401 - 410 of 772 results

  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... approach, and conclusions (see above). Keep original files in a separate folder: You will often use...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...that was temporarily used. Just be sure that the original code remains in tact! Take notes Comment in ...

  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...insert targeted mutations through homologous recombination. To further increase the utility of CRISPR/Cas9...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...Size is also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...

  3. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...cloning, waiting, and repeating before you can finally dive deep into generating the data you’re excited...cost $155.25 in reagents and consumables today. Finally, this doesn’t account for the most valuable component...

  4. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

  5. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...time). Other illumination strategies, such as 2-photon-excitation, selective plane illumination, TIRF or ...experiments on a single day on a stable system. A final piece of advice is to measure the excitation power...

  6. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...yellow, or magenta, depending on the hairpin combination bringing the total number of individual colors...Adding an additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase...15! They also envision using CRISPRainbow in combination with gene editing. CRISPRainbow requires very...

  7. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...available (below). However, on November 22nd a preliminary injunction against the FLSA updates was granted...inconsistencies across geographical regions When we originally started gathering this information, many assumed...to pursue academic work, and to enhance the sustainability of the scientific enterprise by reducing the...

  8. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...ligation assay (PLA). The clever combination of FUNCAT and PLA PLA was originally published by the Söderberg...16590564. PubMed Central PMCID: PMC222790. 6. Buxbaum, Adina R., Bin Wu, and Robert H. Singer. "Single β-actin...

  10. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...or awaiting a final publication in a journal.  Preprints can be one tool in a combination of strategies... Addgene ID for their deposited plasmids in the final journal publication or an updated preprint, further...
Showing: 401 - 410 of 772 results