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  1. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...aborted fetus. HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented...293 is known for reproducible results. It’s also widely available, which can make for a seamless transition...

  2. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...make them decide to read (or listen or watch) further.  Second, the introduction needs to provide enough ...

  3. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...CRISPOR, a tool to help you design, pick, and close guides for Cas9 and Cpf1. This tool supports over 250 ...Mechanisms of precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi...

  5. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  8. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...available for binding change as well. If this change hides the epitope that the antibodies used to recognize...bind to the epitopes that are available on the outside of the cell. The same protein may have different...

  9. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...cells to take up protein directly? It’s not a new idea, but it comes with a few caveats. The most common...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it...

  10. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... schedule tools How to write a lot: A practical guide by Paula J. Silvia  This book has tips for how to...suggestions. De-jargonizer The De-jargonizer can identify jargon in your writing. While it’s not possible...

  11. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...control AAV inventory to give scientists access to a wide array of promoters and fluorophores for validating...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  12. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ...

  14. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et... protein. This technique is very similar to the widely used FRET, but it eliminates the need for an excitation...

  15. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...? Here’s a list of some important variables to consider when choosing an inducible promoter. Experimental... (Tet) On Expression Systems at Addgene Cre-lox Guide and Plasmids     ...

  16. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...inhibits over 18,000 human genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  18. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...messengers. Optical control of kinase activity can provide greater spatiotemporal resolution than pharmacological...vanishing, and pa= photoactivation) domains on either side of the active site of a constitutively active MEK1...

  19. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...the respective hairpin binding domain. Pairs of identical hairpin sequences specify the primary colors blue... colors and a white composite. gRNAs with two identical hairpin binding domains produce blue, green, or...
Showing: 801 - 820 of 889 results