We narrowed to 847 results for: PLE

1. Future of Research Conference - Remarkable Opening Session

   Type

   Blog Post

   Published

   Oct. 7, 2014, 3 p.m.

   ...Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to be in academia to make...

2. 9 Tips to Achieve Success in Academia

   Type

   Blog Post

   Published

   April 1, 2014, 5:33 p.m.

   ...multidisciplinary one.” Cepko strongly emphasizes that people should “follow their passions, be happy, and match...

3. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

   Type

   Blog Post

   Published

   Feb. 20, 2014, 4:57 p.m.

   ...their demonstrated application offer proof-of-principle that CRISPR-Cas9 can be used to target any sequence...

4. Hot Plasmids - October 2022

   Type

   Blog Post

   Published

   Oct. 6, 2022, 1:15 p.m.

   ...  Multiple Authors...

5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

   Type

   Blog Post

   Published

   June 11, 2020, 1:34 p.m.

   ...increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics...

6. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

   Type

   Blog Post

   Published

   May 21, 2020, 1:15 p.m.

   ...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction...

7. Bringing Sustainable Practices to the Lab: Recycling

   Type

   Blog Post

   Published

   Feb. 14, 2019, 2:35 p.m.

   ...packaging (no coated tissue culture plates, for example). Check the link for more details on what they ...

8. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

   Type

   Blog Post

   Published

   July 16, 2019, 12:59 p.m.

   ...spontaneous swimming and escape reflex. These traits, coupled with short generation times and high fecundity,...

9. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

   Type

   Blog Post

   Published

   June 11, 2019, 1:16 p.m.

   ...your search to particular types of images. For example, by selecting “Tools” and then clicking the “Usage...

10. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type

    Blog Post

    Published

    June 19, 2018, 1:38 p.m.

    ...arg2 ultrasound signals. This also means that multiplexing is possible if bacteria are sequentially exposed...

11. CASTing Off for New Shores in Human Genome Editing

    Type

    Blog Post

    Published

    June 10, 2025, 1:15 p.m.

    ...2017). Type I CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type ...

12. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type

    Blog Post

    Published

    March 15, 2018, 12:59 p.m.

    ...Overview of Cas9 nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation inactivates the...

13. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type

    Blog Post

    Published

    Feb. 1, 2018, 3:07 p.m.

    ...Snapgene desktop software allows us to quickly align multiple sequences at the same time. This means we can ...

14. Some Like it Hot: Thermostable GeoCas9

    Type

    Blog Post

    Published

    Sept. 14, 2017, 12:40 p.m.

    ...editing. See figure 4 in Harrington et al for more examples of GeoCas9’s editing activity in HEK293’s. GeoCas9...

15. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    Published

    Jan. 30, 2014, 3:29 p.m.

    ...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...

16. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ... 8 amino acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein...

17. Meet the New Addgene Board Members!

    Type

    Blog Post

    Published

    April 28, 2020, 1:14 p.m.

    ...Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has been involved in...

18. Fluorescent Biosensors for Measuring Autophagic Flux

    Type

    Blog Post

    Published

    Jan. 22, 2019, 2:41 p.m.

    ...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...

19. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type

    Blog Post

    Published

    Nov. 8, 2018, 3:47 p.m.

    ...synthetic vs. natural gene are essential and complementary tools in a time where biological engineering...

20. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...