We narrowed to 842 results for: RING
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TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....Graduates with Degrees in Science, Technology, Engineering and Mathematics, Washington, DC: US Government...
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Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....interactions to remain stable (or to be stabilized) during cell lysis and lysate fractionation. Important ... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog PostPublishedAug. 1, 2023, 1:15 p.m....correctly assembled phage genomes, it required a very stringent selection process and benefited from a longer ... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....week. This is where our new process will begin. Partnering with seqWell, we are using the plexWell technology... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....plasmid from being cut and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....scientists will contact you if any issues arise during the QC process. As the depositing scientist, you... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....Rho-independent terminators, which include naturally occurring terminators, such as T7 and rrnB, as well as engineered... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m.... you can always change your compensation values during the analysis portion of your experiment. So, as... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....kDa). This system also requires less genetic engineering than some of the other systems reviewed here.... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....type-specificin vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....that the fusion protein localizes properly by comparing the signal from the fusion itself to the wild-... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....2021. After validating this method through monitoring GFP degradation, this protein compartment was... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....if the plasmid will be within my lab's budget? Ordering: Check the Price of course! The price of some ... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....there are many factors that need to be considered during the planning process. For CRISPR experiments, the... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....Quick Guide to All Things Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....the expression level in a specific system (by measuring the fluorescence intensity); and 2) to visualize... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....genomic regions." Journal of bioscience and bioengineering 108.5 (2009): 446-449. PubMed PMID: 19804873... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m.... Mary Gearing... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For...