We narrowed to 837 results for: RING
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TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....scientists will contact you if any issues arise during the QC process. As the depositing scientist, you...
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Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....Rho-independent terminators, which include naturally occurring terminators, such as T7 and rrnB, as well as engineered... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....type-specificin vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....2021. After validating this method through monitoring GFP degradation, this protein compartment was... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m.... you can always change your compensation values during the analysis portion of your experiment. So, as... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....kDa). This system also requires less genetic engineering than some of the other systems reviewed here.... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....that the fusion protein localizes properly by comparing the signal from the fusion itself to the wild-... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....if the plasmid will be within my lab's budget? Ordering: Check the Price of course! The price of some ... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....there are many factors that need to be considered during the planning process. For CRISPR experiments, the... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....Quick Guide to All Things Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang et al. (J Virol. 1988... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m....the expression level in a specific system (by measuring the fluorescence intensity); and 2) to visualize... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m.... Mary Gearing... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....genomic regions." Journal of bioscience and bioengineering 108.5 (2009): 446-449. PubMed PMID: 19804873... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....requires animal sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....cross the blood-brain barrier to reach the brain. Delivering the CREATE AAV library via IV screens for capsids... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....tubes help to guarantee that no samples get lost during their journey. Take the time to track your samples... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....FLAG) are cleaved by specific proteases without requiring the addition of an independent cleavage recognition... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....pseudotyping and is not HIV-derived. Get Tips on Titering Your Lentivirus Concerning safety of lentiviral... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....non-functional hedgehog exhibit abnormal segmentation during embryogenesis. Typically, regularly spaced denticles...