We narrowed to 856 results for: TOR

1. Cre-ating New Methods for Site-specific Recombination in Drosophila

   Type

   Blog Post

   Published

   May 12, 2015, 1:32 p.m.

   ...is toxic to proliferating cells. A Cre-estrogen receptor ligand binding domain-fusion can prevent this ...

2. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

   Type

   Blog Post

   Published

   Aug. 13, 2014, 3:49 p.m.

   ...this purpose? What led you to it? Howarth: Our collaborator Uli Schwarz-Linek at St. Andrews discovered ...

3. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

   Type

   Blog Post

   Published

   Feb. 20, 2014, 4:57 p.m.

   ...Using CRISPR/Cas to Edit Disease Out of the Genome History of CRISPR Cas - A tale of survival and evolution...

4. Year of the Camelid: Antibody Style

   Type

   Blog Post

   Published

   May 9, 2024, 1:15 p.m.

   ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

5. Antibodies 101: Affinity Tags

   Type

   Blog Post

   Published

   Sept. 19, 2023, 1 p.m.

   ...Ready to start tagging? We are here to help with vectors, protocols, antibodies, and more!   Resources ...

6. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ...nick distance). Both D10A and H840A are potent editors, but their indel profiles vary. In the PAM-out ...

7. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

   Type

   Blog Post

   Published

   Feb. 13, 2018, 2:51 p.m.

   ...study how differential access to transcriptional regulatory elements is controlled during development using...

8. Management for Scientists: Managing vs. Leading

   Type

   Blog Post

   Published

   March 31, 2015, 4:08 p.m.

   ...of leadership. Perhaps one of your advisors or mentors has urged you to work on developing your “leadership...

9. Management for Scientists: Giving Feedback

   Type

   Blog Post

   Published

   Feb. 6, 2015, 2:46 p.m.

   ...is possible in your eyes so their confidence is restored. A good manager does not hold grudges. Never...

10. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ...was originally derived from jellyfish (Aequorea victoria) and can serve as an epitope tag as well as a ...

11. Antibodies 101: Epitope Availability

    Type

    Blog Post

    Published

    Jan. 11, 2022, 2 p.m.

    ... understand, to the best of your ability, what factors may be affecting the protein’s configuration in...

12. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type

    Blog Post

    Published

    June 22, 2021, 1:15 p.m.

    ...to gain entry into human cells via human ACE2 receptors. In their assay, the pseudoviruses express a luciferase...

13. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    Published

    June 17, 2021, 1:17 p.m.

    ...May 13, 2021 The original AraC transcriptional regulator Their system is based on the AraC protein that...

14. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    Published

    June 9, 2016, 2:30 p.m.

    ...advantageous if you’re working with small capacity viral vectors such as AAV. Base removal RNA-guided CRISPR enzymes...

15. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type

    Blog Post

    Published

    Sept. 15, 2015, 2:30 p.m.

    ...rare diseases,” said Claudia OuYang, BeHEARD Co-Director in a release. The research teams studying four...

16. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ... arms for your gene of interest into the FP-SEC vector using Gibson assembly. This construct will serve...

17. Another Pathway into Cells: iTOP

    Type

    Blog Post

    Published

    June 23, 2015, 8:37 p.m.

    ...limitations of packaging size associated with viral vectors. For these reasons, iTOP, especially in tandem ...

18. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    Published

    May 21, 2019, 12:10 p.m.

    ...to small fluorescence changes of the donor and acceptor after proteolytic cleavage. Secondly, fluorescence...

19. 25 Work From Home Tips for Scientists

    Type

    Blog Post

    Published

    March 19, 2020, 1:15 p.m.

    ...radio reporter challenge, and code.org has coding tutorials for all ages. Organize your data and prepare ...

20. Summer SciComm Series: A PhD in Science Communication

    Type

    Blog Post

    Published

    Aug. 23, 2022, 1:15 p.m.

    ...poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all...