We narrowed to 811 results for: lars

1. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ...seed growth on antibiotic-containing media) A similar process occurs in nature; Agrobacterium tDNA insertion...

2. The Importance of a Fun Workplace: Company Culture at Addgene

   Type

   Blog Post

   Published

   Sept. 3, 2015, 2:30 p.m.

   ... you are planning, dangle the carrot (or more popularly- cookies). 3. Do something outside of your ordinary...

3. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

   Type

   Blog Post

   Published

   July 7, 2020, 1:15 p.m.

   ...Quantification of CRISPR/Cas9-mediated Genome Editing. Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org...

4. Save Time with Transient Plant Leaf Transformations

   Type

   Blog Post

   Published

   July 25, 2019, 12:03 p.m.

   ...fused to a protein of interest to elucidate the subcellular localization of your protein of interest. Find...

5. Transferable Skills Guide: Creativity

   Type

   Blog Post

   Published

   Jan. 18, 2022, 2:15 p.m.

   ... of encouraging dissent, the next tip may be particularly relevant to you. Elect a devil’s advocate. This...

6. Lab to Office Culture Shock

   Type

   Blog Post

   Published

   Feb. 5, 2019, 1:04 p.m.

   ...cross-team projects at Addgene, I find that my vocabulary improves on a weekly basis. There will be meetings...

7. How to Be an Excellent Trainee

   Type

   Blog Post

   Published

   Sept. 26, 2023, 1:15 p.m.

   ...something. It can be intimidating to ask for clarification at first, but in the end everyone wins. Your...

8. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...based repressors are useful when knocking out a particular gene isn’t feasible. For example, temporary repression...

9. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ...created in an effort to increase translation in a particular plant species or cell type (Belhaj et al., 2013...

10. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ...models remain more difficult to make. For example, large insertions, like a fluorescent protein knock-in,...

11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ...need our A549 cells to express Cas9. In these particular experiments, we generated A549 cells stably expressing...