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Addgene

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We narrowed to 772 results for: INA

Showing: 411 - 420 of 772 results

  1. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...questions the conclusion of inter-homologue recombination was released. This blog has not been updated...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...embryos to find those that do not carry a given dominant disease-causing gene, or that carry only one copy...

  2. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers were trying...treatment process. The authors went on to show that combination treatment with multiple drugs reveals even smaller...heterogeneity to drug discovery and the development of combination therapies.         References 1. Hyo-eun, C. ...

  3. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ... Editing, and that meant rereading many of the original papers reporting various prime editing tools. ...your curiosity, we encourage you to check out the original papers for more details. You can find many software...prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22), 5635-5652...

  4. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking the right ligand...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...our chemogenetics poster! This blog post was originally written by Maya Peters Kostman in May 2020 and...

  5. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ... the protein or structure of interest. For a C-terminal tag, as shown in the example above, we inserted...would be transcribed and translated. For an N-terminally tagged protein, we used the same strategy, inserting...tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional...

  6. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...dCas9. Within a few rounds of cell division, the original methylated strand is diluted to undetectable amounts...dCas9 so that it may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-...L, Li C, et al. (2018) OGT binds a conserved C-terminal domain of TET1 to regulate TET1 activity and function...

  7. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...used for this study at Addgene which include a C-terminal deletion in the spike protein to improve expression...pseudoviruses to determine how effective sera from vaccinated individuals are at neutralizing the different...

  8. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...is ideal because it eliminates the potential of human error or cross contamination between samples. With... Shannon Rinaldi...

  9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...immunogenic profile. AAV was first discovered as a contaminant of adenovirus preparations. It is an icosahedral...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene... responses to adeno-associated virus and its recombinant vectors." Gene therapy 10.11 (2003): 964-976....

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... incident, and the sterilization of women in marginalized communities have created a legacy of distrust...included taking extra coursework and attending seminars in bioethics, public health, science policy, and...advantage of their academic environment and attend seminars, symposia, or courses in social, health or policy-related...
Showing: 411 - 420 of 772 results