We narrowed to 821 results for: LAS

...100,000 different plasmids available from the repository (currently at 98,276 plasmids as of April 8, 2021...re now sharing over 100,000 reagents including plasmids, ready-to-use viral preps, pooled libraries, kits... 2021) 1.5 million plasmids shared in total (currently at 1,484,507 as of April 8, 2021) 5,000 depositing...addgene.org homepage! You can track the number of plasmids right below the search bar and more stats further...

... tag expressed on a plasmid, allowing for in vivo biotinylation through a plasmid-based biotin ligase....CRISPR guide Find CRISPR plasmids by function Find recently deposited plasmids ...which can easily be cloned into a gRNA expression plasmid. Experimental use Since the initial report describing...currently no cure (Campbell et al., 2018). Find plasmids from the Fujii lab! Conclusions CRISPR-mediated...

...(cap) expressing plasmids is generated. Diversity is introduced into the cap plasmid library by inserting...Caltech stepped up to the challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method to...region of the cap gene. In this special CREATE cap plasmid, loxP sites flank the downstream polyA sequence...sequence. When viruses produced from this plasmid pool are injected into an animal with cell-type-specific Cre... do these AAVs transduce the CNS and PNS? Both plasmids infect the PNS or the CNS much better than the...

... great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow... where variable chains are secreted into the periplasmic space to be oxidized and form disulfide bonds...antibodies can be produced through a single or dual-plasmid transfection, depending on whether the heavy and...and light chain genes are contained to one plasmid or if they’ve been separated onto two. You’ll first...first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to collect ...on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies...

...fluorescent conjugates into cells. Fortunately, over the last 20 years clever chemists and biochemists have developed...interaction between a bisarsenical dye ligand (e.g., FlAsH, ReAsH) and a short genetically encoded tetracysteine...off a flurry of activity and the majority of the classic small molecule fluorophores, such as rhodamines...small-molecule labeling techniques have focused on this classic, net-neutral dye scaffold due to its established...enhance the brightness and photostability of the classic rhodamines and their analogs without sacrificing... Generate Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent...

...transforming both fusion plasmids, or mating two single haploid yeast fusion plasmid transformants into a ...system as a robust and popular lab tool. In the classic two hybrid system (also called the interaction ...reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example...positive control to ensure that your setup works. The last thing you want is to run your lovely library screen...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our...

...efficiency of plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and... https://doi.org/10.1038/nature24644 Glaser A, McColl B, Vadolas J (2016) GFP to BFP Conversion: A Versatile...upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al., 2016). For TREE they engineered a BFP variant...Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ...

...Cas7-11 might be phylogenetically classified as the more complex Class I systems that contain multiple ...the complexity of its ancestors. They classified the system as Class I, Type III-E, and then used this information...editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development and discovery The ...Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells, and ...

...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...pseudotyped protein is encoded on the envelope plasmid which contains a promoter, the envelope gene, and...scientists encode the spike protein on the envelope plasmid. Image from Crawford et al., 2020.   Broad tropism...

...comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the protein ...construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific promoters...individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters...sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular application...