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We narrowed to 814 results for: LAS

Showing: 411 - 420 of 814 results

  1. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ... Genome Engineering Guide Plant Plasmids and Resources CRISPR Plasmids and Resources ...recent years. TnpB nucleases represent an exciting class of compact genome editors, typically only around...for ωRNA (reRNA) expression (see Figure 1). Both plasmids contain a hygromycin resistance gene as the plant-selectable... 2–3 positive colonies, grow them, and extract plasmids. Confirm the correct guide insertion by Sanger...primer. Step 7: Transformation Your confirmed plasmid is now ready for Agrobacterium-mediated, biolistic...

  2. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...realized we could apply the lessons learned from our plasmid lab to develop a reliable and robust high quality...offer many resources for producing vectors from plasmids — including the protocols we use in our lab — ...while viral vectors are not as easy to ship as plasmids, they still have a global reach. From 2016–2023...vectors in our repository are produced from only 413 plasmids containing a genetic payload. While AAV9 and AAV1...having reliable ready-to-use vectors made from plasmids in the Addgene catalog would be broadly useful...conditions. Sharing our experiences Addgene’s plasmid repository gave us the experience to launch a high-quality...

  3. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...efficiency of plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and... https://doi.org/10.1038/nature24644 Glaser A, McColl B, Vadolas J (2016) GFP to BFP Conversion: A Versatile...upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al., 2016). For TREE they engineered a BFP variant...Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ...

  4. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...aptus, meaning “to fit”. SELEX could be used to classify DNA aptamers as well, and over the course of the...detection assay. In 1999, Grate and Wilson paired laser cleavage and an RNA aptamer that bound the compound...difficult-to-study RNA-modifying enzymes, such as the RNA demethylases FTO and ALKBH5. Their ease of production have...27446908. PubMed Central PMCID: PMC4923196. 8. Sassolas, Audrey, et al. “Optical detection systems using...

  5. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...Cas7-11 might be phylogenetically classified as the more complex Class I systems that contain multiple ...the complexity of its ancestors. They classified the system as Class I, Type III-E, and then used this information...editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development and discovery The ...Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells, and ...

  6. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...pseudotyped protein is encoded on the envelope plasmid which contains a promoter, the envelope gene, and...scientists encode the spike protein on the envelope plasmid. Image from Crawford et al., 2020.   Broad tropism...

  7. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the protein ...construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific promoters...individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters...sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular application...

  8. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species.   Specificity...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety of species...additional RNA-binding transcriptional activator. Plasmids for activating target genes in a variety of species...systems Addgene carries a variety of Cas9 containing plasmids that have been optimized for expression in different... plants, drosophila, worms and mammals (Browse plasmids by model organism, here).  More information on... resources on the Addgene website Find CRISPR Plasmids Catch up on your CRISPR background with our CRISPR...

  9. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve...scientists (and our growing collection of 25,000+ plasmids from 1,600+ labs around the world!) that have ...inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and... and get practical tips on how to use the plasmids in your own lab. Summaries of the latest technologies...the bench, with a special focus on cloning and plasmid-based technologies. Commentary about scientific...

  10. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking...about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene....Podcasts Find Great Educational Content in our Plasmids 101 Series Additional Resources on Addgene.org...
Showing: 411 - 420 of 814 results