We narrowed to 884 results for: ica

... at Sanofi Pharmaceuticals. This post highlights a rare disease, Fibrodysplasia ossificans progressiva...surpassed 60,000 views for the month of September! Historically we do better in September than in the summer...or you can’t quite remember the hazards of that chemical in your obscure growth medium, these apps can ...Binding Proteins," was contributed by guest blogger Jessica Sacher from the University of Alberta and describes...the post for the full details and many thanks to Jessica for all of her hard work and attention to detail...can find the rest of our September posts in chronological order here: Four Things I Learned at the 10... a Lab and a Project That’s it for now. As I indicated in the opening paragraph, I’m currently on my ...

...: 18px;font-family: helvetica;","color":"#FFFFFF","variant":null,"font":"helvetica","style":"font-family...button_font":{"size":18,"styles":{"font-family":"helvetica","font-style":"normal","font-weight":"normal",...font-family: helvetica; font-style: normal; font-weight: normal; text-decoration: none","font_set":"DEFAULT"}... into viral vectors that can be administered systemically (retro-orbitally, RO; or intracerebroventricularly...intracerebroventricularly, ICV) or locally (stereotaxically, STX) to the brain. Figure 2: Selection, prep, and testing...developed, funded, and shared has no doubt changed drastically over the past few decades. But no matter how ...and transgenic mouse lines for genetic access to cortical cell types. Cell. https://doi.org/10.1016/j.cell...

...are subject to excision and ssDNA cleavage by canonical base excision repair enzymes. At the same time...2019). TREE is a real-time, fluorescent based identification system for the isolation of base-edited cell...targeted by a cytidine deaminase base DNA editor. Specifically the BFP mutant (BFPH66) contains a histidine...GFP positive cells isolated with TREE have a significantly higher frequency of genomic base pair edits ...GFP positive cells can be sorted and have a significantly higher frequency of base edits of interest than...robust induction of GFP fluorescence confirming the efficacy of GO as a base-editing reporter. As the initiation...markers, and other enzymes such as Cre-recombinase drastically expanding the base-editing reporter toolbox. ...

...were less significant than when non-targeting guides are used as controls. Less significant p-values lead... approach for identifying genes involved in a biological process. These screens provide an alternative...highly effective, are affected by low on-target efficacy, non-specific toxicity, and off-target effects...calling a gene a hit when really it’s not). In practical terms, this means that using safe-targeting guides...guides and may more accurately determine the significance of hits in non-growth screens. Off-target cutting...of on-target activity. However, there was no significant difference in depletion of ricin sensitizer genes...and do not have a major reduction in on-target efficacy. Results from Morgens et al. show that genome-...

...cancer. These later stages of a cancer diagnosis indicate, for Stage 3, that the cancer is large in size...size and may have spread while Stage 4 indicates that the cancer has spread from where it originated to ...asbestiform minerals such as erionite, are the only scientifically proven causes of the disease. There is no question... question on this point within the medical and scientific communities, and the link between asbestos and.... New techniques are currently being tested in clinical trials to detect mesothelioma earlier than ever...earlier, thus improving the patient’s prognosis significantly, but also to monitor those who may be at greater... working to fight the cancer.  Beyond early modifications to standard treatments and early detection, ...

...Addgene ID number, your publication will be listed on our website as a “Publication Citing this Plasmid” ...upon other researchers being able to find their publications in the first place. As a curator of the thousands...make it easier for you to find the plasmids and publications relevant to your research interests. We do this... this by promoting citation, supporting sophisticated search, and curating plasmid collections. Addgene's...number as well as the reference for its original publication. To help facilitate this process, we explain ...fruits of your labor and won’t have to repeat the modification. This will save both time and money. A quick...whether the plasmid has been used in your system. Sophisticated search You can search for specific plasmids ...

...The biomedical field is often concerned with understanding the cause of diseases and how to treat those...optimization is performed to take the drug to the pre-clinical and human trial phases.   So how does CRISPR factor...put into them in those later stages. Target identification Not all diseases are fully characterized or...simple pathology. In these cases, preliminary identification of potential targets by CRISPR can accelerate...enables researchers to select and generate physiologically relevant models. In complex diseases, it can...patient mutations which are variants of unknown significance – mutations with unclear effects on disease ...standard is to test the drug on models which are genetically proficient and deficient for the target. CRISPR...

...any DNA editing functionality Collecting preclinical and clinical data about a disease or therapeutic Side-by-side... ReFigure. Reading and exploration including replications and experiments resulting in “negative data”...small selection of data found in full-length publications. As early career researchers, we wondered how...performance as in this antibody ReFigure Analyzing replications as in this ReFigure about whether Natronobacterium...reagent performs well, or you might publish a replication experiment that you conducted as part of setting...papers as they are not included in traditional publications. By submitting your data to a repository (Figshare...-registration, preprints, data, software, micropublications) in grant proposals. ReFigures are one such...

...If you use a kit for DNA purification or if you use a DIY purification protocol, you might have noticed... mutations in single stranded DNA undergoing replication.   DNase on the other hand can digest and degrade...degradation during plasmid DNA extraction and purification from E. coli is the presence of the protein ...most common E. coli strains used for plasmid amplification are endA negative, meaning the gene has been...chelating agents that can be introduced in the purification protocol to remove endonucleases activity. For...directly use your DNA preparation in any downstream application involving enzymatic reactions, a valid alternative...temperature for a short period, and there are indications that Tris buffer is better than water in these...

...gene at once. Therefore, applications that require simultaneous modification of all alleles of a gene,...frequency three orders of magnitude higher than identical targeting vectors delivered through transfection... recommends designing homology arms with the modification of interest, ordering them from a gene synthesis...with a homologous repair template. It is this application - the creation of sequence-specific knockins ...clones, making it unsuitable for most in vivo applications.  Third, even in the most optimized tissue-culture...tissue culture based approaches that require modification of only a single allele of a gene, such as the...efficiently deliver large repair templates for applications that require large gene fusions. The current...