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We narrowed to 814 results for: LAS

Showing: 421 - 430 of 814 results

  1. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...other scientists save resources by sharing plasmids Find plasmids at Addgene ...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...reuse, reducing the need for the production of new plastic.  Recycling and reuse is not limited to the office... however. We participate in many styrofoam and plastic recycling programs in the lab as well. For a more...

  2. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ... for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect... Surface labeling is particularly useful for classifying and sorting cells by identifying lineage markers...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live cells... performed on cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule...Addgene Addgene's Antibody Guide Addgene's Antibody Plasmid Collection Resources on the Addgene blog Antibodies...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life expectancy and quality for...infected cells. Although ART-treated patients lack plasma HIV-1, they are at increased risk for other chronic...reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription of...26538064. PubMed Central PMCID: PMC4633726 Find SAM plasmids used in this publication at Addgene. Kaminski...PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. Wang Z, ...Addgene’s newly updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ...

  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...problem of HDR vs. NHEJ entirely. The first two classes of base editors were cytosine base editors (CBEs...or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair,...producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system...off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second... highlighted in this article.  Publication Plasmids   Highlights Komor et al., 2016 BE1, BE2, BE3...background with our CRISPR Guide Browse CRISPR plasmids for base editing Find validated gRNAs ...

  5. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...other scientists save resources by sharing plasmids Find plasmids at Addgene ...This is the last post in a blog series on sustainable practices in the lab, where I’ll list some easy...bottle instead of buying yourself a big stack of plastic cups. By initiating little changes in ourselves... do the same. Long-term lab conservation goals Lastly, I think it’s a good balance to have both short-term...

  6. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...and beyond. Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors...biosensors plasmids. Our most exciting milestone of 2022, however, is arguably the launch of our fast-...knowledge and research opportunities. I have spent the last 9 months quietly learning about Addgene’s past and...

  7. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...AAV receptor (not strictly required)2 Human fibroblast growth factor receptor 1 (FGFR1)3 αVβ5 and α5β1...pseudotypes are described with two numbers separated by a slash: the first number refers to the genome, and the ..., et al., 2006). AAV2 capsids are available as plasmids in the repository and can be selected as a serotype..., et al., 2006). AAV8 capsids are available as plasmids in the repository, as part of our in-stock viral...retinal cells (Issa et al., 2023). AAV9 packaging plasmids are available at Addgene. AAV9 vectors are not...Request Browse all in-stock AAV preps Browse all AAV plasmids ...

  8. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...for Your Research View our Hot Plasmid Pages to See the Latest Plasmid Technologies ... drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As described in...It’s also possible you could make use of these plasmids in living animals, to explore pathways that confer...Be Used in Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on...

  9. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...for at least a couple of decades. Green is the classic color, but fluorescent proteins are available in...in 2011 and then in another Nature Methods paper last year. The key to their bacterial phytochome-derived... could see deeper.” Adding to the toolbox In the last year, Verkhusha added two far-red light photoactivatable...the researchers described in Nature Communications last year, “The capability to control spectral properties...containing substantial autofluorescence background.” Just last month in Scientific Reports, Verkhusha’s team reported...

  10. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...interest endogenously and transfect them with a plasmid encoding your protein of interest. Then you should...target protein in a Western blot. You acquire a plasmid encoding your protein of interest, known as Gene... In parallel, you transfect cells with another plasmid not encoding this protein, which we will call Negative...band from the cells transfected with the other plasmid. Your antibody works! Multiple (Independent) Antibodies...Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ...Designing a recombinant validation experiment? Find plasmids here!  References Baker M (2015) Reproducibility...
Showing: 421 - 430 of 814 results