We narrowed to 821 results for: LAS

...comprehensive CRISPR guide Find CRISPR plasmids and resources Find CRISPRa plasmids ...genes can be relatively laborious because multiple plasmids/viruses are needed to deliver all the protein ...construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under neuron-specific promoters...individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters...sensitive applications such as CRISPR screening. The plasmids used by the Qi Lab for this particular application...

... Genome Engineering Guide Plant Plasmids and Resources CRISPR Plasmids and Resources ...recent years. TnpB nucleases represent an exciting class of compact genome editors, typically only around...for ωRNA (reRNA) expression (see Figure 1). Both plasmids contain a hygromycin resistance gene as the plant-selectable... 2–3 positive colonies, grow them, and extract plasmids. Confirm the correct guide insertion by Sanger...primer. Step 7: Transformation Your confirmed plasmid is now ready for Agrobacterium-mediated, biolistic...

...great choices. Check out our SpCas9 containing plasmids, optimized for a variety of species.   Specificity...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety of species...additional RNA-binding transcriptional activator. Plasmids for activating target genes in a variety of species...systems Addgene carries a variety of Cas9 containing plasmids that have been optimized for expression in different... plants, drosophila, worms and mammals (Browse plasmids by model organism, here).  More information on... resources on the Addgene website Find CRISPR Plasmids Catch up on your CRISPR background with our CRISPR...

...Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene View All Fluorescent...aptus, meaning “to fit”. SELEX could be used to classify DNA aptamers as well, and over the course of the...detection assay. In 1999, Grate and Wilson paired laser cleavage and an RNA aptamer that bound the compound...difficult-to-study RNA-modifying enzymes, such as the RNA demethylases FTO and ALKBH5. Their ease of production have...27446908. PubMed Central PMCID: PMC4923196. 8. Sassolas, Audrey, et al. “Optical detection systems using...

...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve...scientists (and our growing collection of 25,000+ plasmids from 1,600+ labs around the world!) that have ...inside scoop from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and... and get practical tips on how to use the plasmids in your own lab. Summaries of the latest technologies...the bench, with a special focus on cloning and plasmid-based technologies. Commentary about scientific...

...other scientists save resources by sharing plasmids Find plasmids at Addgene ...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...reuse, reducing the need for the production of new plastic.  Recycling and reuse is not limited to the office... however. We participate in many styrofoam and plastic recycling programs in the lab as well. For a more...

...the bay area to create a plasmid rap video for us (If you’re new to plasmids, we highly recommend checking...about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support from Addgene....Podcasts Find Great Educational Content in our Plasmids 101 Series Additional Resources on Addgene.org...

... for panel design. Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect... Surface labeling is particularly useful for classifying and sorting cells by identifying lineage markers...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live cells... performed on cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule...Addgene Addgene's Antibody Guide Addgene's Antibody Plasmid Collection Resources on the Addgene blog Antibodies...

...Antiretroviral therapy (ART) can virtually eliminate plasma HIV-1, improving life expectancy and quality for...infected cells. Although ART-treated patients lack plasma HIV-1, they are at increased risk for other chronic...reservoir, researchers have previously used histone deacetylase (HDAC) inhibitors to increase transcription of...26538064. PubMed Central PMCID: PMC4633726 Find SAM plasmids used in this publication at Addgene. Kaminski...PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at Addgene. Wang Z, ...Addgene’s newly updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ...

...problem of HDR vs. NHEJ entirely. The first two classes of base editors were cytosine base editors (CBEs...or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair,...producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com. The BE3 system...off-target activity.  Find CRISPR base editing plasmids at Addgene Improving cytosine base editing scope...byproducts likely resulted from excision by uracil N-glycosylase (UNG) during base excision repair. Adding a second... highlighted in this article.  Publication Plasmids   Highlights Komor et al., 2016 BE1, BE2, BE3...background with our CRISPR Guide Browse CRISPR plasmids for base editing Find validated gRNAs ...