We narrowed to 783 results for: INA

...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...members all say the same thing and one panel member dominates the discussion? Or maybe the one that goes way...front of a crowd. The moderator will then develop a final list of questions for the actual event, based on...

... an approach called Simultaneous Tracking of Recombinase Activation and Clonal Kinetics (STRACK) to study...epithelial cells, liver, CNS (limited penetration), retina, hematopoietic cells, and immune cells. B) AAV11...postsynaptic AMPA receptor and is endocytosed, the Cre recombinase payload activates a floxed reporter gene (genetically...

...Figure 1). Through this method, we found key determinants of prime editing efficiency such as the length....0 while accounting for some of the inherent inaccuracies of computational prediction models. In addition...efficiencies using sequence features and DNA repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org...

...2014 to 2023. As you can see, nucleases, the original CRISPR tool, remain the most popular deposited...Read our recent publication, “The Expanding Dissemination and Distribution Patterns of Diverse CRISPR ..., R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution Patterns of Diverse CRISPR ...

...don’t get done if there is not a clear deadline. Finally check-in regularly, make yourself available, and...that one on one discussion can be confidential. Finally, provide timely feedback. We often associate feedback...manner. If someone’s performance or behavior is inadequate, you need to address it as soon as possible for...

...increased sensitivity allows for a more confident determination of copy number, albeit within a range of RQ ...trends? How confident do you need to be in your determination to move forward with your experiment?  Complexity...real-time polymerase chain reaction methods for the determination of amplification of Plasmodium falciparum multidrug...

...the end product of PCR temperature cycling. The final PCR product is often visualized on a diagnostic ... dNTPs and other reagents to run low during the final cycles which would slow or stop PCR amplification...statistics to determine the concentration of the original template DNA in the sample.  Addgene currently...

...generated info for your own purposes, or delete the originals if desired. It works as a whole or in part. For...ranging between 0 and 1. The cutting frequency determination (CFD score) is used to measure specificity of...re-binding. Lastly, the sequence inserted by HDR may inadvertently re-create a match for the active guide. With...

...active during M and early S phases, when HDR is inactive, and the balance of NHEJ, HDR, and MMEJ repair...longer the homology, the more efficient the recombination, but with longer homology arms comes more time...which has been difficult to modify due to NHEJ’s dominance over HDR in this species. As CRISPR technology...

...fragment to the N- and C-terminal end of each PIP and empirically test which combination provides the best fluorescent...cellular functions, including ubiquitinoylation, kinase signaling, integrin signaling, and transcription...