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We narrowed to 772 results for: INA

Showing: 431 - 440 of 772 results

  1. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...active during M and early S phases, when HDR is inactive, and the balance of NHEJ, HDR, and MMEJ repair...longer the homology, the more efficient the recombination, but with longer homology arms comes more time...which has been difficult to modify due to NHEJ’s dominance over HDR in this species. As CRISPR technology...

  3. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...scientist. When our elementary school needed a new coordinator for the annual Math & Science Night, I took the...It’s through the PTA that I recently started coordinating the annual Math & Science Night, a chance for...contacting our school district’s science curriculum coordinator with minimal luck, but eventually the 8th grade...

  4. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...T. R. (2015). Stability and compatibility of recombinant adeno-associated virus under conditions commonly...Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct...Identification of factors that contribute to recombinant AAV2 particle aggregation and methods to prevent...

  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...for some applications, the benefit it offers is marginal. Fortunately, amp can easily be replaced with ...NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene confers resistance to several.../1475-2859-11-56 Trastoy, M. O., Defais, M., & Larminat, F. (2005). Resistance to the antibiotic Zeocin...

  6. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...labs and institutions. For example, search for “Melina Fan” and you’ll be able to go directly to a page...In this example we’re going to be looking for recombinant monoclonal antibody plasmids from the James Trimmer... ‘Vector Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses....

  7. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...are binary gateway vectors and can be used for promoter swapping and generating N and C terminally tagged...4. Nakagawa, T. et al. (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation ...

  8. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...template control PCR reaction for detecting contamination is also a good idea.    Analyzing PCR product...positive clones (+) compared to negative clones. Finally, orientation-specific primers (3) give the same...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

  9. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...virulent or antibiotic resistant bacteria, and inappropriate antibiotic usage selects for resistant bacteria...including Chipotle and Panera Bread have pledged to eliminate the use of antibiotics by their suppliers, and... DSB occurs on a plasmid, the plasmid will be eliminated from the bacterium, which may also induce cell...

  10. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...signal sequence (gene start) and a transcription termination/polyadenylation signal sequence (gene end). A...encoded in separate (and thus smaller) plasmids. Finally, having four separate plasmids allows researchers...it reaches the gene end signal, where it will terminate transcription and polyadenylate the mRNA. These...
Showing: 431 - 440 of 772 results