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We narrowed to 814 results for: LAS

Showing: 431 - 440 of 814 results

  1. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...associated publication for the specifics of your chosen plasmid. Types of biosensors When choosing a biosensor...11,000-fold. FRET biosensors are another common class of biosensors. Blue flame “cameleon” calcium sensors...compartment such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes...

  2. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial Collections...-old boy named AJ Gonzales who suffers from Fibrodysplasia ossificans progressiva (AKA FOP). This terrible...two ways. First, Addgene is distributing ACVR1 Plasmids from a variety of research labs and hopes that...pathways that impinge upon FOP to deposit their plasmids with Addgene and join us in the fight against ...

  3. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...been cloned into plasmids. Because their sequences are known and encoded on a plasmid, they can be easily...are identical for antibodies within certain classification groups called isotypes.  Why does isotype matter...recombinant antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived ...regions of a specific antibody are cloned into a plasmid backbone containing all constant regions, just ...

  4. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo...the kidney—either endothelial, epithelial, or fibroblast. However, Frank Graham showed HEKs display many...T-antigen. This allows them to replicate viral plasmids with the SV40 origin of replication. To generate...cell lines are susceptible to contamination by mycoplasma and bacteria, but HEKs are also particularly ...

  5. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...resource: Recombinant Antibodies! In addition to plasmids and viral vectors, you’ll now be able to order...et al., 2015). All of Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant...other recombinant affinity reagents, share your plasmids via Addgene! Email [email protected] to learn...support, we have enabled scientists to easily share plasmids with each other. Together, we can transform the...Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

  6. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...the newest plasmid you requested from Addgene...the plasmid that will help you run that last additional...experiment requested by Reviewer #3. But why isn’t the plasmid here yet? A quick look at your order status on ...of intellectual property (as inventions, ideas, plasmids) among universities and other research institutions...

  7. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...This construct will serve as the repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins...proteins. The repair template and Cas9-sgRNA plasmid is then injected into the germline of young adult ....115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other strategies...10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable and...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

  8. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...), 339-350 (2015). PubMed. Find the Plasmids @Addgene: Plasmids for CRISPRi are described here: http...constructs to be introduced simultaneously in a single plasmid. The functionality of the RNA scaffolds follows...http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et al. Cell  (2015) can be found here...https://www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found...

  9. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...Looking for Optogenetics Plasmids? Plasmids for Optogenetics Research (Addgene) Plasmids from Ed Boyden's lab...conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of them very popular... lab Sign up to receive email alerts when new plasmids from the Boyden lab become available. Visit Ed...

  10. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...are single cell, eukaryotic organisms formally classified as fungi. They reproduce by either fission or..., yielding higher production power from a given flask volume. What’s even better? K. phaffii is highly...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...
Showing: 431 - 440 of 814 results