We narrowed to 821 results for: LAS

...other scientists save resources by sharing plasmids Find plasmids at Addgene ...This is the last post in a blog series on sustainable practices in the lab, where I’ll list some easy...bottle instead of buying yourself a big stack of plastic cups. By initiating little changes in ourselves... do the same. Long-term lab conservation goals Lastly, I think it’s a good balance to have both short-term...

...and beyond. Last year, we distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors...biosensors plasmids. Our most exciting milestone of 2022, however, is arguably the launch of our fast-...knowledge and research opportunities. I have spent the last 9 months quietly learning about Addgene’s past and...

...for Your Research View our Hot Plasmid Pages to See the Latest Plasmid Technologies ... drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As described in...It’s also possible you could make use of these plasmids in living animals, to explore pathways that confer...Be Used in Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on...

...for at least a couple of decades. Green is the classic color, but fluorescent proteins are available in...in 2011 and then in another Nature Methods paper last year. The key to their bacterial phytochome-derived... could see deeper.” Adding to the toolbox In the last year, Verkhusha added two far-red light photoactivatable...the researchers described in Nature Communications last year, “The capability to control spectral properties...containing substantial autofluorescence background.” Just last month in Scientific Reports, Verkhusha’s team reported...

...interest endogenously and transfect them with a plasmid encoding your protein of interest. Then you should...target protein in a Western blot. You acquire a plasmid encoding your protein of interest, known as Gene... In parallel, you transfect cells with another plasmid not encoding this protein, which we will call Negative...band from the cells transfected with the other plasmid. Your antibody works! Multiple (Independent) Antibodies...Antibodies blog post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ...Designing a recombinant validation experiment? Find plasmids here!  References Baker M (2015) Reproducibility...

...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...associated publication for the specifics of your chosen plasmid. Types of biosensors When choosing a biosensor...11,000-fold. FRET biosensors are another common class of biosensors. Blue flame “cameleon” calcium sensors...compartment such as the nucleus, mitochondria, endoplasmic reticulum (ER), vacuole or cellular membranes...

...CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial Collections...-old boy named AJ Gonzales who suffers from Fibrodysplasia ossificans progressiva (AKA FOP). This terrible...two ways. First, Addgene is distributing ACVR1 Plasmids from a variety of research labs and hopes that...pathways that impinge upon FOP to deposit their plasmids with Addgene and join us in the fight against ...

...been cloned into plasmids. Because their sequences are known and encoded on a plasmid, they can be easily...are identical for antibodies within certain classification groups called isotypes.  Why does isotype matter...recombinant antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived ...regions of a specific antibody are cloned into a plasmid backbone containing all constant regions, just ...

...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo...the kidney—either endothelial, epithelial, or fibroblast. However, Frank Graham showed HEKs display many...T-antigen. This allows them to replicate viral plasmids with the SV40 origin of replication. To generate...cell lines are susceptible to contamination by mycoplasma and bacteria, but HEKs are also particularly ...

...the newest plasmid you requested from Addgene...the plasmid that will help you run that last additional...experiment requested by Reviewer #3. But why isn’t the plasmid here yet? A quick look at your order status on ...of intellectual property (as inventions, ideas, plasmids) among universities and other research institutions...