We narrowed to 884 results for: ica

... and 15/17 morphologically aligned. Documentation of the network that initiates biological reprogramming...unique barcodes for each TF help streamline identification. While the Zhang lab controlled their multiplicity...single TF was delivered to each cell, future applications could deliver multiple TFs to each cell, allowing...processes. For help with library transformation and amplification, refer to the Zhang lab’s previously published...published protocol and its relevant modifications on the pooled library webpage. Disease models Good disease...generate, in part because of difficulty with replicating the specific dysregulation in the disease. For...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome Library...

...ll be talking about the ins and outs of this classification system, including its history, biology, and...repeatable phenomenon, Gram began using it as a classification system. Eventually, any bacteria that retained...gramstain.html Smith, A. & Hussey, M. Gram Stain Protocols. American Society for Microbiology (2005). https://asm.org... or pink. Purpose Gram staining can be used in medical settings to determine the general type of bacteria...permeable to different classes of antibiotics. The classification system is also useful in a laboratory setting...bacteria type. The gram positive and negative classification system is incredibly helpful in visualizing...stepping into a lab.   Anna Kamens is a science communication summer intern at Addgene and a rising third-...

...Technology and its Application in Genetic Diseases: A Review.” Current pharmaceutical biotechnology. https...2020.  “CRISPR Gene Therapy: Applications, Limitations, and Implications for the Future.” Front. Oncol...several treatments are currently being tested in clinical trials. It is speeding the process of the discovery...the technology in different labs for different applications.” Li’s team noted that CRISPR/Cas9 has been ...cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence editing efficiency...with size limitations. In addition, non-viral applications are also possible through the use of nanoparticles...

...Harvard Medical School and the University of Toronto. He is an author of 20+ scientific publications and ...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known... a collection of 96 plasmids on a plate) will typically have a wide range of input quantities. This variation...samples. As mentioned above, one of the primary technical benefits of plexWell™ is that normalized libraries... proprietary chemistry). This turns out to be critically important for getting adequate sequencing depth...computational biologist with a passion for solving critical sequencing problems. He received his PhD in Bioinformatics...

...MSDS for a lab chemical of choice. Now, I can’t guarantee every reagent in your chemical closet is in this...lab notebook. Lab Archives is a multipurpose application that allows you to share your data with colleagues...directions including which buffers to use and that indicates if a sequential digest is needed. The enzyme finder...enzyme sequence resource is universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety always...experiments. Hence, I’m including this MSDS app. The Chemical Safety Data Sheets app has a search function that... if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS book, this is a...AMINO is a very simple app that packs important biological information, amino acid abbreviations, and structures...

... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...does one stand out amongst a pool of successful applicants and get chosen to work in his or her lab of choice...environment? Does the group expect each other to be physically present at certain times during the day? We asked... next.” Connie Cepko looks for people who are dedicated and interested in science. “Postdoc hires are ...history in science. Potential graduate students typically go through a rotation, which reveals their working...job satisfaction (especially for scientists and technical people) is linked to engagement and access to ... "chaotic" and offer less micromanaging, they typically offer more flexibility in choosing direction of...

...target specific tissues, even when administered systemically, without causing disease? Recombinant adeno-...receptors and tissue tropism. AAV5 AAV5 is the most genetically distinct AAV. Though AAV5 is one of many serotypes...vessel barriers. It is especially popular for applications targeting liver tissue. In addition, it has ...the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al., 2023). Additionally...treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by...enhances astrocyte-directed transduction. Nature Communications, 14(1), 3792. https://doi.org/10.1038/s41467.... A. (2023). Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), ...

...to the right indicates a higher signal, or upregulation, and a peak to the left indicates a lower signal...anything to the right of it, marking it as GFP+. Quantification for such graphs could simply be the percentage...case for you, switching to a contour plot should indicate where the two populations diverge. The smear ...place your gate based on the dot plot would be complicated.  Figure 2: (A) a smeary dot plot showing...Figure 4 compared to the dot plot in Figure 2. Quantification using MFI makes it easier to identify and study...Research Assistant in Arlene Sharpe’s lab at Harvard Medical School.   More resources on the Addgene blog Antibodies...addgene.org Addgene's Antibody Guide rAb Affinity Purification Protocol Video rAbs in the Addgene repository...

...Following up its 2018 iGEM debut - centered around a prototypical methane biosensor - IISER Bhopal is back in ...psychrophilic enzymes for milk processing, fruit juice clarification, meat tendering, and oligosaccharide synthesis...enzymes implies that even a minute quantity can significantly reduce the operational time for a given process... from their native conditions of ~0°C, which drastically affects their stable folding and efficient functioning...chaperones (the Cpn 10/60 isolated from Oleispira antarctica, and the P. haloplanktis Trigger Factor), that...Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and ...cultivating a nuanced understanding of the societal ramifications of laboratory science, and how it may best be...

...emphasizing the importance of critical thinking, persistence, teamwork, and communication. And as a program focused...BioBuilderClub teams’ biological engineering designs and ideas ranged from a biological breathalyzer to pesticide... Dr. Natalie Kuldell from MIT’s Department of Biological Engineering. Built primarily from her 20 years...experiential learning. “Students may forget the technical details they hear in their classes,” she said,...and teachers to apply engineering principles to biological systems. Today, what started in Cambridge with...Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid summer internships...