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...as links to the Zhang Lab's Nature Protocols Publication describing them here. References 1.Ran, F. Ann...

...Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley,...

...right path. The frequent Zoom meetings helped me communicate my ideas. I was able to express my visions for...

...approach." Proceedings of the Royal Society B: Biological Sciences 272.1577 (2005): 2117-2126. PubMed PMID...

...course of scientific research. What was once a complicated process of requesting and sharing plasmids has...

...complements the auxotrophy (i.e., synthesizes the critical amino acid). Many common E. coli strains used ...

...National Academy of Sciences of the United States of America, 114(35), E7358–E7366. https://doi.org/10.1073/...

...easier to ship back. Be that person! If you use serological pipettes from Corning®, Falcon®, or Axygen®, ...

... a Cas9 nickase and two gRNAs.  For nickase applications, a common question is: how should the gRNAs ...

...providing an avenue for temporary/reversible CRISPR modification. In addition to these subpages, our updated ...

...could also be used with in vitro molecular bio applications that require high temperature reaction conditions...

... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...

...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...

...requests for scientists doing almost every type of biomedical science research. Our work accelerates and enhances...

...coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet...

... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...

...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...

... of the entire plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing...

... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...

...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...